Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,502	C→T	T128I (ACT→ATT)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,502	0	C	T	100.0%	119.2 / NA	33	T128I (ACT→ATT)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/11);  total (22/11)

TGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGG  >  NC_000913/3486262‑3486742                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 tgGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGaa                                                                                                                                                                                                                                         >  2:321063/1‑250 (MQ=255)                        gagGGTAAAGAAATGATCCTCTCCTATCTGAATCCGGGTGATTTTATTGGAGAACTGGGCCTGTTTGAAGAGGGCCAGGACCGTAGCGCCTGGGT‑ACGTGCGCAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGTCATTCTGATGCGTTTGTCTGCACAGATGGCGCCTCGTCTGCAAGTCATTTCAGAGAGAGTGGGCACACTGGCGTTCCTCg                                                                                                                                                                                                                  >  1:311124/1‑250 (MQ=255)                                             tcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGcac                                                                                                                                                                                             >  1:69464/1‑250 (MQ=255)                                                           gggTGATTCTCTTGGCGAAATGGGCCTGTTTGAAGCGGGCGAGGAAAGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGCAATTTCGTACAAACAATTTCGCCAATTGATTCAGGTCAACCCGGGCATTGTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCAGCATTGCACAGACTCTGCTGAATc                                                                                                                                                                              >  2:127351/1‑251 (MQ=255)                                                                 ttttATGGGCGAACTGGGCCTGTTTGAAGAGGGCAAGGACCGTAGGGC‑TGGGTTACGTGAGAAACCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCa                                                                                                                                                                         <  1:81544/250‑1 (MQ=255)                                                                                gggCCGGTTGGAAGAGGGCCAGGAACGTAGCGAATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGGCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa                                                                                                                                                         <  1:321063/251‑1 (MQ=255)                                                                                      gTTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTc                                                                                                                                                   >  2:4978/1‑251 (MQ=255)                                                                                           aaGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCg                                                                                                                                              <  1:4978/251‑1 (MQ=255)                                                                                                  ccAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGt                                                                                                                                        >  1:283806/1‑250 (MQ=255)                                                                                                   cAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGCAGTGGCTGAAATTTCGTACCAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGCCGGTAt                                                                                                                                      >  2:279332/1‑251 (MQ=255)                                                                                                   cAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTAt                                                                                                                                      <  1:6357/251‑1 (MQ=255)                                                                                                          ggaGCGCATGGGA‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATc                                                                                                                               <  1:305680/249‑1 (MQ=255)                                                                                                          gTAGCGCATGGTT‑CCGTGCAAAACCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATc                                                                                                                               <  1:25650/251‑1 (MQ=255)                                                                                                                              gAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGaa                                                                                                             >  2:95923/1‑250 (MQ=255)                                                                                                                               aaaaCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTACAGAGAAAGAGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGATCTGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAAt                                                                                                           >  1:138537/1‑251 (MQ=255)                                                                                                                                 aaCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAGGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                                                                                                         <  2:114180/251‑1 (MQ=255)                                                                                                                                      ccTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg                                                                                                    <  2:74039/251‑1 (MQ=255)                                                                                                                                       cTGTGAAGAGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCCGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTc                                                                                                      >  2:203122/1‑248 (MQ=255)                                                                                                                                           gAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                                                               >  2:247815/1‑251 (MQ=255)                                                                                                                                           gAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGCATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                                                               >  1:168177/1‑251 (MQ=255)                                                                                                                                            aaGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCGCGACGTGACGTGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTc                                                                                              <  2:317667/251‑1 (MQ=255)                                                                                                                                               tGGCTAAAATTTCGTACAAAAACTTTCGCCAATTGATTCAGGTCAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAACACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGc                                                                                           >  1:254424/1‑251 (MQ=255)                                                                                                                                                ggCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                                                                          >  1:319524/1‑251 (MQ=255)                                                                                                                                                         ttCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAACTTGGTCAGATTGTCGGCTGTTCTCGTg                                                                                 >  2:90587/1‑251 (MQ=255)                                                                                                                                                            gTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGCAATTGGTCAGATTGTCGGCTGTTCTCGTGaa                                                                               >  1:84059/1‑250 (MQ=255)                                                                                                                                                                    aaTTTCGCCAATTGATTCAGGTAAACCCGGACATGCTGATGCGTTTGTCTGCACAGATGGCGGGTCTTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCGCGACTTGACGGGCCGCATTGCACAGTCTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCTCCCTGACGGTATGCAAAGCAAGATTACCCGTCAGGAAATTGTTCAGATTGTCGGCTGGCCTCGTGAAACCGTGGGa                                                                      <  2:311019/251‑1 (MQ=255)                                                                                                                                                                                       ggTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTgg                                                   >  2:35690/1‑251 (MQ=255)                                                                                                                                                                                                      tCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTg                                     >  2:321237/1‑250 (MQ=255)                                                                                                                                                                                                       cTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCATTTCCGAGCAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAt                                   >  1:189797/1‑251 (MQ=255)                                                                                                                                                                                                                        acaGATGGCGCGTCGTCTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAAACAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATATCCGCACACGGtcaa                   >  1:216292/1‑247 (MQ=255)                                                                                                                                                                                                                          agaaggCGCGTCGTCTGCAAGTCATTTCAGAGAAAGGGGGCAACCTGGCGTTCCTCGACGTGACGAGCCGCATTGCGCAGACTCTGCTGAATCTGGCAATAAAACCAGTCGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCGGGAAATTGGTCAGATTGTCGGCTGTTCTCATGAAACCGATGGACGCCTTCTTAAGAATCTGGAAGATCATAACCTGATCTCCGCCCACGGTaaacc                 >  2:174233/5‑248 (MQ=255)                                                                                                                                                                                                                                        cTGCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACgg  >  2:177697/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCAAGTCATTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACgg  <  2:101079/249‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 TGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGG  >  NC_000913/3486262‑3486742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑