Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	694,941	G→A	L5L (CTC→CTT)	miaB ←	isopentenyl‑adenosine A37 tRNA methylthiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	694,941	0	G	A	100.0%	119.1 / NA	35	L5L (CTC→CTT)	miaB	isopentenyl‑adenosine A37 tRNA methylthiolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/21);  total (14/21)

GCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTT  >  NC_000913/694710‑695187                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGGGTTCAGCAGCAGCCCATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACt                                                                                                                                                                                                                                     <  1:230049/251‑1 (MQ=255)      cGACGCCGATAATCAGGGCTGGATTCTTCTCTTGTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGAGCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTGTTAATATGAAGTTTTTTGGTCATCGACTTGctc                                                                                                                                                                                                                                <  2:317209/251‑1 (MQ=255)        acgacGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGTTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCCCGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCCGATCGGCTATCTTCGATGAATCGTACTCTTTCATCTGACAGCCCCAGGTTTTAACATGAAGTTTGTTGGTCATCGACTTGCTCtt                                                                                                                                                                                                                              <  1:241767/247‑1 (MQ=255)         cGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTg                                                                                                                                                                                                                             <  1:221386/251‑1 (MQ=255)                      gTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGcc                                                                                                                                                                                                                >  1:116720/1‑251 (MQ=255)                            aTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAAt                                                                                                                                                                                                          <  1:121441/251‑1 (MQ=255)                                      tttAACAGTTTCCAGCGACCCAACTGATGGAAGTCTTTTTCCTGCGCCTTCTCGCGGATTGAGCAGGTGTTCAGCATCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCTAGGAATGCAGGgcg                                                                                                                                                                                                  <  2:52683/249‑1 (MQ=255)                                                tCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAAt                                                                                                                                                                                       <  1:124421/250‑1 (MQ=255)                                                  cAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCt                                                                                                                                                                                    >  1:309483/1‑251 (MQ=255)                                                   aGCGACCAAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGTTCATCGACTTGCTCTTGCGAATTAGTAGCCAGGAATGAAGGGCGCATAGTGTAATGCtt                                                                                                                                                                                   >  2:181077/1‑251 (MQ=255)                                                              tGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTtg                                                                                                                                                                        >  1:160694/1‑251 (MQ=255)                                                                           tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATg                                                                                                                                                             >  2:267390/1‑249 (MQ=255)                                                                                  ggCCTTCTCGCGGATTGGGCAGGTGTTCAGCAGCAGCACAACCGCTTCTGCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGGATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTAGGAGCGTTATc                                                                                                                                                    <  2:58011/250‑1 (MQ=255)                                                                                            cgGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATTCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAgg                                                                                                                                          <  2:212892/251‑1 (MQ=255)                                                                                                               gcagcagcaCATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACt                                                                                                                       <  2:288985/251‑1 (MQ=255)                                                                                                                                tcttcCGCCACGTCGGTCAGTTGATAGCCGTGTGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAAc                                                                                                      >  2:236118/1‑251 (MQ=255)                                                                                                                                         aCGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGAtt                                                                                             <  1:138401/251‑1 (MQ=255)                                                                                                                                               gTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGTCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAACCCTGTAAGCTTAAAGCATATTGTTAACAGGATGATTGACCAt                                                                                       <  2:295547/251‑1 (MQ=255)                                                                                                                                                 cAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATg                                                                                      >  1:226934/1‑250 (MQ=255)                                                                                                                                                         aGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATcaa                                                                             <  1:308448/251‑1 (MQ=255)                                                                                                                                                               gggtggCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACg                                                                       <  1:267390/251‑1 (MQ=255)                                                                                                                                                                ggtggCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACgg                                                                      >  1:13886/1‑251 (MQ=255)                                                                                                                                                                    gCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGGTCATCTGACAGCCCCAGGTTTTAAAATGAAGTTTTTTGGTCATCGACTTGCGCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTCGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAAt                                                                  <  1:44364/251‑1 (MQ=255)                                                                                                                                                                      aTCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTg                                                                <  2:226934/251‑1 (MQ=255)                                                                                                                                                                          agcagATCGGCCATCTTCGATGAATCTTACTCGTTCATCCGACAGCACCAGGTTTTAATAGGAAGTTTGTGGGTAATCGACGTGCTCTTTCGAAATAGTAGCCAGGCATGCAGGGCGCATAGTGTAATGCTTGGCTGCCGTTGTGATAAGTATGCGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATGGCGAACAGGATGATTGACCATGACAAATCAACCACCGGAAATGGCCAt                                                            <  2:61684/251‑1 (MQ=255)                                                                                                                                                                           gcagATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCAtt                                                           <  1:86132/251‑1 (MQ=255)                                                                                                                                                                            cagATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTg                                                          <  2:260424/251‑1 (MQ=255)                                                                                                                                                                                 cGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTcggc                                                     >  1:54863/1‑251 (MQ=255)                                                                                                                                                                                     cATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTcggcgg                                                   >  2:214542/1‑249 (MQ=255)                                                                                                                                                                                     cATCTTCGATGAATCGTACGCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGTCAAATCAACCAACGGAAATTGCCATTGTCGGCggag                                                 <  2:319644/251‑1 (MQ=255)                                                                                                                                                                                                          gTTCATCTGACAGCCCCCGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTgg                            >  2:149712/1‑251 (MQ=255)                                                                                                                                                                                                                  gACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCt                        <  2:249946/247‑1 (MQ=255)                                                                                                                                                                                                                              ttttACTATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTCACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCAc        >  2:53664/1‑251 (MQ=255)                                                                                                                                                                                                                                 tAATATGAAGTTTTTTGGTCATCGACTTGCTATTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCACTGGGGCTGGCACAGCaagg      >  1:315816/1‑247 (MQ=255)                                                                                                                                                                                                                                    tatGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGAttt  >  1:162461/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTT  >  NC_000913/694710‑695187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑