Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,279,276	C→G	F121L (TTC→TTG)	kbaZ →	putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,279,276	0	C	G	100.0%	137.8 / NA	38	F121L (TTC→TTG)	kbaZ	putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (19/19);  total (19/19)

AACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCACCACGCCGG‑ATGCCGCCCGCGCCACGCTGGAAGCC  >  NC_000913/3279036‑3279522                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        atcGCCGTTACTGAATAAACAAACCTCCAATCAGGTTGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATcc                                                                                                                                                                                                                                               <  2:119584/249‑1 (MQ=255)               ttGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTgcagc                                                                                                                                                                                                                                 >  1:265451/1‑251 (MQ=255)                                cAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCtt                                                                                                                                                                                                                                          <  1:94737/225‑1 (MQ=255)                                  ggTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATc                                                                                                                                                                                                              <  1:322544/251‑1 (MQ=255)                                      gcccAGTTCGTGGGTTAGACCGGAAGGACGCCCGCCGATTTTCGCGGCTTTGTTTGGCAGCTCGCCGACTCGTTTAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGAt                                                                                                                                                                                                          <  2:147622/249‑1 (MQ=255)                                               ggcggTTATTCCGGAATGCCGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAAc                                                                                                                                                                                                 <  2:265451/251‑1 (MQ=255)                                                                             tttCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGccc                                                                                                                                                                    <  1:194193/250‑1 (MQ=255)                                                                                       tttgttgtCAGCTCGCCGACTCGTGGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCCCCCGTCTTGCGaa                                                                                                                                                          <  2:46675/246‑1 (MQ=255)                                                                                         gtttgtCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTACAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGCTGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAgg                                                                                                                                                       >  2:117616/1‑250 (MQ=255)                                                                                                    cGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGgaataa                                                                                                                                             <  2:226935/250‑4 (MQ=255)                                                                                                    cGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAGTCTGGGTGTTGACCATCTGGGTCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACTTTGCGGCAGGATTGAAAAAAAGCCACCTTGATTGCAGCATGTCCTGTCAGGACGAGCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCTTCTGTCGAGAGTGGCGgaaga                                                                                                                                              <  2:19074/249‑1 (MQ=255)                                                                                                      ccGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAAcc                                                                                                                                          >  1:133323/1‑251 (MQ=255)                                                                                                            cGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTc                                                                                                                                      <  1:266221/249‑1 (MQ=255)                                                                                                                     tCCCGCAGGATGCGTTGATTCTGGGTGTTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCACTGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGAGATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTAGCGGAAGaaaacgtctttgaacacatt                                                                                                                           >  2:241602/1‑234 (MQ=255)                                                                                                                        cGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCACTGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACCCTTTGGc                                                                                                                        >  1:294712/1‑251 (MQ=255)                                                                                                                         gCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGGGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTgtcg                                                                                                                       >  2:158419/1‑248 (MQ=255)                                                                                                                           aGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGaa                                                                                                                     <  2:271804/251‑1 (MQ=255)                                                                                                                             gATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGCAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATAGTGGCTGAACGCGCCGCCCGTCTGGCGAAAACGGCGGAAGAAACCTGTCTTGACCAATTTGGCGAAGc                                                                                                                   >  2:268087/1‑251 (MQ=255)                                                                                                                                               ggtgACCATCTGGGGCCAAACCGCTGGCACAACCGGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTTATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCTAAGCCGATCTGGAGTATGTCa                                                                                                  <  2:294712/250‑1 (MQ=255)                                                                                                                                                      aTCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACCCTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTAcc                                                                                          >  1:29674/1‑251 (MQ=255)                                                                                                                                                           gggCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGAACTGGAGGATGTCATTGGTACCGAAGt                                                                                     >  1:232075/1‑251 (MQ=255)                                                                                                                                                              ccAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTGTGGCGAAGCCGATCTGGAGTAGGTCATTGGTACCGAAGTGcc                                                                                  <  1:283837/251‑1 (MQ=255)                                                                                                                                                                       tGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGGGTATGTCATTGGTACCGAAGTGCCGGTACCTgg                                                                         >  2:134404/1‑251 (MQ=255)                                                                                                                                                                                            cAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCCCCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCTCGCATGAAACCTTaa                                                     <  2:188515/250‑1 (MQ=255)                                                                                                                                                                                            cAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAg                                                    <  1:59033/251‑1 (MQ=255)                                                                                                                                                                                                    ggCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTGTGGCGAAGCCGATCTGGAGTATGTCATGGGTACCGAATTGCCGGGACCTGGCGGCGCGCATGAAACCAGAAGCGAGCTg                                             <  2:141313/250‑1 (MQ=255)                                                                                                                                                                                                      ccAATGCCGATGATTTGATGAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCGTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCGGGCTTGAACACTTTGTCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTTGCGGCGCGCATGAAACTTTAAGTGAGTTGGCg                                          <  2:165199/251‑1 (MQ=255)                                                                                                                                                                                                       cAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAACAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGCTGACATCGTGGCTGAACGCGCCGCCCGACTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACCCTTTCGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAAACTTAAGAGAGCTGGCgg                                         >  1:293329/1‑251 (MQ=255)                                                                                                                                                                                                        aaTGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGGATGTCATTGGTACCGAAGTGCCGGGACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCggg                                        >  2:130124/1‑250 (MQ=255)                                                                                                                                                                                                                  atttgattAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACCCTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCa                                                                >  1:110704/1‑217 (MQ=255)                                                                                                                                                                                                                  atttgattAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCa                                                                <  2:110704/217‑1 (MQ=255)                                                                                                                                                                                                                          aaaaGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGCTCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCCCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACCCTTTGGCGAAGCCCATCTGGAGGATGTCATTGGTACCGAAGTGCCGGTACCCGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCCCCACGCCGG‑ATgcccc                     >  2:259773/1‑249 (MQ=255)                                                                                                                                                                                                                              gCTACGTTGCGGCAGGATTGAAACAAATCCACCTTGATTGCAGCATGTCCTGTAAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGCATGTCATTGGTACCGAAGAGACGGTACCTGGCGGCGCGCATGAAAACTTAAGCGAGCTGGCGGGCACCACGCCGG‑ATGccgcccgc                 >  1:77086/1‑251 (MQ=255)                                                                                                                                                                                                                               cTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCACCACGCCGG‑ATGccgcccgcc                >  1:3377/1‑250 (MQ=255)                                                                                                                                                                                                                               cTACGATGCGGAAGGGTTGAAAAAAATCCACCTTGTTTGCAGCATGTCCTGTCAGGCCGATCCGCATCACTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGACTTGAACACTTTGGGGAAGCCGCTCTGGGGTATGTCATTGGTACCGAAGTGCCGGCAACTGGCGGCGCGCATAAAACCTTCAGCGACCCGGCGGTCCCCCCGCCGG‑GTGccgcccgcc                >  2:165267/1‑250 (MQ=255)                                                                                                                                                                                                                                     tGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTTGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGGACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCCCCACGCCGG‑ATGCCGCCCCCCCCACGc          >  2:64976/1‑251 (MQ=255)                                                                                                                                                                                                                                          cAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCACCACGCCGG‑ATGCCGCCCGCGCCACGCTGGaa     <  1:201646/251‑1 (MQ=255)                                                                                                                                                                                                                                             gATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGGCTTGAACACTTTGGGGAAGCCGGTCTGGCGTATGGCATTGGTACCGAAGTGCCGGGACCTGGCGGCCCGCATGAACCCTTAAGCGAGCTGGCGGTAACCACGCCGGGAT‑CACCCCGCCCCACGCTGGAAGcc  >  2:67264/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        AACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTGAACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCACCACGCCGG‑ATGCCGCCCGCGCCACGCTGGAAGCC  >  NC_000913/3279036‑3279522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑