Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	107.9 / NA	33	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/16);  total (17/16)

GCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTT‑ACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGT  >  NC_000913/3387806‑3388283                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    gcgGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAt                                                                                                                                                                                                                                      <  1:268028/251‑1 (MQ=255)        aaCCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCt                                                                                                                                                                                                                               <  1:63705/251‑1 (MQ=255)         aCCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTg                                                                                                                                                                                                                              <  1:86688/251‑1 (MQ=255)           cGGCCAGCACCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTc                                                                                                                                                                                                                            >  1:275105/1‑251 (MQ=255)                    cccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTCCAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATCCGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGcc                                                                                                                                                                                                                   >  1:153353/1‑251 (MQ=255)                        cGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGc                                                                                                                                                                                                               >  1:59074/1‑251 (MQ=255)                             aCGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAAGGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTgg                                                                                                                                                                                                           <  2:137222/250‑1 (MQ=255)                                        atgcGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCt                                                                                                                                                                                                <  2:56345/247‑1 (MQ=255)                                        aTTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCCGCTg                                                                                                                                                                                               >  2:78319/1‑251 (MQ=255)                                                   cGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGc                                                                                                                                                                                    <  2:91347/251‑1 (MQ=255)                                                              aGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGGGAAAGCCAACAAACAgc                                                                                                                                                                         >  1:283092/1‑251 (MQ=255)                                                                       aaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGAAATGATGATCACCAGTCCGGCAATACCGCCAATACCTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGACTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACACTCGCCGCTGTCAGTACCGCCCAGAGTGGCGTTTCCAGCTGGAAGTGAAAGCccacacaaaccgccattcc                                                                                                                                                                 >  2:258881/1‑236 (MQ=255)                                                                        aCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGGACa                                                                                                                                                                >  1:121506/1‑250 (MQ=255)                                                                              ccAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCgg                                                                                                                                                         <  1:297328/251‑1 (MQ=255)                                                                                 gATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCg                                                                                                                                                      >  2:263504/1‑251 (MQ=255)                                                                                           acCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTAc                                                                                                                                            >  1:93459/1‑251 (MQ=255)                                                                                             cAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATTTGCGGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCATCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTTAAAGTCAACAAAGAGCGCCAGGACAATGGCGGTCGCCAGTTTTACCg                                                                                                                                          <  2:310988/251‑1 (MQ=255)                                                                                                     tcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCCCCAGGTTTACCGCAAAGCg                                                                                                                                   >  1:73378/1‑250 (MQ=255)                                                                                                      caATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGaa                                                                                                                                 <  1:5110/251‑1 (MQ=255)                                                                                                             ggTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACCATGGCGGTCGCCCGGTTTACCGCAAAGCGAATCTGTTg                                                                                                                          >  1:271201/1‑251 (MQ=255)                                                                                                              gTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTgg                                                                                                                         <  1:249886/251‑1 (MQ=255)                                                                                                                tcgcgGATCATCGCAATGATGACCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCCCGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGGCAGTACCGCCCAGCGTGGCGTTTACAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGt                                                                                                                        <  1:55967/249‑1 (MQ=255)                                                                                                                     gATCATCGCAATGATGATCACCAGGCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCaa                                                                                                                  <  2:111793/251‑1 (MQ=255)                                                                                                                           cGCAATGATGATCACCAGTCCGGCAATCCAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGgag                                                                                                             <  2:275105/250‑1 (MQ=255)                                                                                                                                 gatgatCACAAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATAc                                                                                                      <  1:284278/251‑1 (MQ=255)                                                                                                                                                  cAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTTCCGCCAAGCGGAAATGGTGGTTAGCAATGGGGAAAAAACCCCTCGTGATTaaaca                                                                                      >  1:178904/1‑247 (MQ=255)                                                                                                                                                              tAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTACCCCAAAGCCAATATGTTGGTTAGCCATGGGGGAAATAACCAACGTGATTAACCAAAccccccccggc                                                                         >  2:6934/1‑241 (MQ=255)                                                                                                                                                                     gCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATTCCCATCGTGATTAACCAAACTCACGCATGCGATggg                                                                   <  2:195507/250‑1 (MQ=255)                                                                                                                                                                                         cACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTT‑ACGGAAGAACGAAt                                             >  1:139832/1‑251 (MQ=255)                                                                                                                                                                                         cACGATAGCGAATAGCGCCAGAATACGGCTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGGGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGAGGTCGCAAGGTTTACCGCAAAGCGAATATGTTTGTTAGCAATGGGGTAAAAACCCATCGTGATTAACCAAACTCCCACAGGCGATGGGCCATTTT‑ACGGGagaaagaa                                              >  2:147081/1‑250 (MQ=255)                                                                                                                                                                                                           cAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCACCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATTGCGGTCGCCAGTTTTACCGCACAGCGCATATGTTGGTTTGCAATGGAGAACAAACCCATCGTGATTCACCAAACTCACCCAGGCGAATGGCCATTTTTACG‑AAAAACGAAACCAGGCTTTTATCGCGa                            >  1:297834/1‑250 (MQ=255)                                                                                                                                                                                                                                cagcaAAGGCCGGACCGGCGTCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAAGGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAAGGGATAAAATACCCATCGTGATGAACCAAACTCACGCAGGCGATGGGCCATTTT‑ACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGAccacca      <  1:35767/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTTCCGCCAAGCGAATATGTTGGGTAGCCATGGCGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTT‑TCGGAAGAAAGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCCCTGt  >  2:167260/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    GCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTT‑ACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGT  >  NC_000913/3387806‑3388283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑