Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,601	(T)5→4	coding (1251/1401 nt)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,601	0	T	.	100.0%	95.2 / NA	21	coding (1251/1401 nt)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/13);  total (8/13)

ATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTA‑TTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATA  >  NC_000913/3025363‑3025835                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            aTGTCCGGCGAACTATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCTATGATTGGCGGCTTCTTCACGACCATTCCCTCGGTAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCa                                                                                                                                                                                                                                 <  2:183854/250‑1 (MQ=255)   gTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGcc                                                                                                                                                                                                                              <  2:228265/251‑1 (MQ=255)         cgtaCCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTAtt                                                                                                                                                                                                                        <  2:228738/248‑1 (MQ=255)             ccATCGCGGTAATGCTGGTTAGCCTCGGCTTAGTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTGCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATg                                                                                                                                                                                                                    <  1:133661/251‑1 (MQ=255)              cATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGt                                                                                                                                                                                                                   >  1:26058/1‑251 (MQ=255)                    ggTAAAGCTGCTTAACCTAGGCTTATTTCCGTTGCTTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGCGCGGCAATGACGTCGATGTTTTCCATGCTTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAACGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGACATTTT‑ACAATATTGCCAGCCTCTATTTATGCATTAGt                                                                                                                                                                                                             >  2:293367/1‑251 (MQ=255)                     gTAATGCGGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATGTG‑AAAATATTGCCAGCCTCTATTTATGTATTAGtt                                                                                                                                                                                                            <  1:114601/251‑1 (MQ=255)                       aaTGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGa                                                                                                                                                                                                          <  1:327481/251‑1 (MQ=255)                                              ttCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCggg                                                                                                                                                                                    <  2:81166/250‑1 (MQ=255)                                                                                                     aaTGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAATGCGGTTTTATTAAATATTTTCCTCCCTGGTGGGTACCGGCAGGAAAACGt                                                                                                                            >  1:75054/1‑251 (MQ=255)                                                                                                                                      aGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAAATTCGATTTTATTAAATATTATCCTCCCTGGGGGGTCCCGACCGGAAAACGTTTTGCCTGGTA‑TTACCTCAGCGGGAGAGATggg                                                                                          >  2:262347/1‑250 (MQ=255)                                                                                                                                       gggATTCGCATCATCATCACCAACGGTTTAAAGCGCCGCGCAACACTTATTGTCGCCACTTATTTAGGTTTAGGCCTTGGTGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCTGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTATTTGCGCGTTTATTATATATTATCCTCCCTGGCGGCTAACGGCAAGAAAACATGTTGCCTGGTA‑tttactcagggggaatgatgg                                                                                           >  2:13495/1‑230 (MQ=255)                                                                                                                                                atcatcatcACCAACGGTTTAAAGCGCCGTGAAACACTTATTGGCGCCACTTCTTTAGGTTTAGGGCTTTGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTA‑TTACCTCAGCGGAAGAGATGGATTAACAGTaa                                                                                <  1:325827/251‑1 (MQ=255)                                                                                                                                                    tcatcaCCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTTTTAAat                                                                                                                                                               >  1:256249/1‑169 (MQ=255)                                                                                                                                                    tcatcaCCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAat                                                                                                                                                               <  2:256249/169‑1 (MQ=255)                                                                                                                                                                              gAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTA‑TTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACg                                                   <  1:225078/250‑1 (MQ=255)                                                                                                                                                                                          gTCGCCAATTCTTTAGGTTTAGGACTTGGAGTCTCATACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCATATTTGTGCTGGCGGGATAACTGCGATTTTATTAAATATTATGTTCACTGGGGGGTATCGACAGGAAAGAGTTCGGCCTGGAAATTAC‑TCAGAGGGAGAGAGGGATTAACAGGAAAGGGGTAAATGATGGCAGGAGAacacaagattaaagaggaa                                       >  1:86775/1‑236 (MQ=255)                                                                                                                                                                                                             taggGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTA‑TTACCTCAGCGGAAGAGAGGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGGACGAGGCAGTTTTATTGATg                    <  2:56149/250‑1 (MQ=255)                                                                                                                                                                                                                      gCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTA‑TTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTAc          <  1:97263/251‑1 (MQ=255)                                                                                                                                                                                                                        gTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTA‑TTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTAGTGATGTCACCCGTAcga        <  1:300981/251‑1 (MQ=255)                                                                                                                                                                                                                               aCGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGTTTTTTTTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAAAGTTTTGCCTGGTA‑TTTCCTCAGCGGAAGAGATGGATTTACAGGAAAGGAGTCAATGATGTCAGGAGAAAACACGTTAAAAGCGGTACGAGGCAATATTTTTGATTTCACCCGTACGATCGATa  >  2:13758/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            ATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGTTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTA‑TTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATA  >  NC_000913/3025363‑3025835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑