Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	41.7 / NA	16	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/8);  total (8/8)

CCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTT  >  NC_000913/1301755‑1302208                                                                                                                                                                                                                                              |                                                                                                                                                                                                                         ccGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTTCCTATTGCTTCTGAAGTTACCGATGTCGACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGcc                                                                                                                                                                                                             <  1:122483/251‑1 (MQ=255)                                                            cGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCAt                                                                                                                                                  >  1:48897/1‑251 (MQ=255)                                                               cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTGCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAc                                                                                                                                                <  2:251293/250‑1 (MQ=255)                                                                aCCAACGGTGCCTATCACTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACGGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATGTGCCTATTGCTTCTGAAGTTACCGATGGCAACCGCTTCCGGAGTGGTGAAAGCGATATGAAGTAGAACAGCATGCCGATCGAATTGTTCCAGAAGCGGAAAAAAGAGATCCCGGACGAAGTTCGCGtggttgcatactg                                                                                                                                              <  2:50985/251‑13 (MQ=255)                                                                aCCAACGGTGCCTAGACCTTAAACGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCTTGATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGAAGTGGTGAAATGGACAGGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGACCCTTACCt                                                                                                                                              <  2:147218/251‑1 (MQ=255)                                                                                                       gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGcc                                                                                                       >  1:131186/1‑251 (MQ=255)                                                                                                            aaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGACAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTc                                                                                                   >  1:161068/1‑250 (MQ=255)                                                                                                               cGTTCTTGAACGCAGCCCGACCTCCTGGAACAACGCGAAAACCGTTATTAACCAGTTACCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTAAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAAc                                                                                                <  2:34840/250‑1 (MQ=255)                                                                                                                            aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGCAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgcg                                                                                  >  1:28650/1‑251 (MQ=255)                                                                                                                            aGCCCGACCTACTGGAACAACGCGAAAACCGTTATCAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTACAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgcg                                                                                  >  1:113821/1‑251 (MQ=255)                                                                                                                                                            tattaACCAGGTACCCTAGTGGCCTATTGCTTCTGAAGTTCTCGAGTTCACCCGGTACCGTAGTGGTGAAATCGTCAGGACGTATACCAGCATGCCGTTCGAATTGTTTCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCGGTGCACTTACTATTAGGAAATTAACAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTATCGCACTGAAACTAGGTATGGACCGCGat                                                   <  2:161068/250‑1 (MQ=255)                                                                                                                                                                aaCCAGGTAACCTATTTGCCGATTGCTTCTGAAGTTACCGATGTAAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGACCTAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATGCCTGTGCACTTACTATTACGAAATAAACACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAt                                              <  2:225541/251‑1 (MQ=255)                                                                                                                                                                             aTTTGCCTATTGCTTCTGAAGTTAACGAAGTCAGCCGCTACCGTAGCGGAAAAATCGACATGACGTATAACAGAATACAGATAGAATTGTTCCAGAAGCTGAAAAAAGAGATACCGAACGAAGTTCACGTTGATCCATACCTGTGAACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGAGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTg                                 >  1:314447/1‑251 (MQ=255)                                                                                                                                                                               ttGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATTACGTATAACAGCAGGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTTTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCCCTGAAACTAGGGATGGACCGCGATATCATTGTTAATAAAGTGa                                <  2:327908/250‑1 (MQ=255)                                                                                                                                                                                      ttGCTACTGAAGATACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCAGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGAATGGACCGCGATATCATTGTTAATAAAGTGAAAgcgc                          >  2:239156/1‑249 (MQ=255)                                                                                                                                                                                                             aaCCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGtt  >  2:3466/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                         CCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTT  >  NC_000913/1301755‑1302208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑