Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,991	G→A	L5L (CTG→TTG)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,991	0	G	A	100.0%	69.7 / NA	23	L5L (CTG→TTG)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

AGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGG  >  NC_000913/3926747‑3927236                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       aGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAAtt                                                                                                                                                                                                                                                 >  2:58376/1‑251 (MQ=255)    gcgGAAGGGTGTTCTTGGGCGCTCTTTAATATAAGGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGTTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTc                                                                                                                                                                                                                                              <  2:126147/251‑1 (MQ=255)                   ttGGCGCTCTTGAATATAAGTCCAGTACACCAGCCCACGTCATAACCGAGCTGCTTCGGGCTGACATCAATCCGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCTGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCt                                                                                                                                                                                                                                 <  2:19256/249‑1 (MQ=255)                   ttGGCGCGCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCGGCTCCGGTCTGACATCAATACGCGCCCCTGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCtt                                                                                                                                                                                                                                <  2:63730/250‑1 (MQ=255)                    tGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCtt                                                                                                                                                                                                                                >  1:63730/1‑249 (MQ=255)                    tGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTa                                                                                                                                                                                                                               <  1:134860/250‑1 (MQ=255)                     ggCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATg                                                                                                                                                                                                                             >  1:49321/1‑251 (MQ=255)                                  ataATGACGATAAAGACGCCAACGTCATTACAGAGCTGCTTCGAGCTGACCTAAAAACACGCCACGCAAATGATCAACGCATGCTTCATTTTCTCTACTCGAAACTGAATCGTCACCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGGGAGCGTATTGCCCATTAATGCGTCCAGGATGCCACGGTCCAGATTGTCG‑CCAAACTAATTTTCAATAGGATTTCCTTATGCGGATTGATGatt                                                                                                                                                                                                                >  2:31650/1‑251 (MQ=255)                                                                   aGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCa                                                                                                                                                                               <  2:286470/251‑1 (MQ=255)                                                                                              gcccccgcactGATCCCAGCCTGCTTCATTTTCTATACTCTTACCTGAATAGTCCACGGACTGACGCCAGATTGTTTTGTCAGTTCGGCGTAAGGGCTGCGCGCATAGACCATTAACGCTTCTAGCATGACATGGTTCAGCTTGTCGATCTAA‑TAATTTTCCATAGGGTTTTTTTATCCGGCTTGATGATTCATTCTATTTTAGCCTTCTTTTTTCATGAATCAAACGTGAGTTAGGCtttttcttatatg                                                                                                                                                    >  1:68064/11‑243 (MQ=255)                                                                                               ccccGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGattg                                                                                                                                                    >  1:303166/1‑247 (MQ=255)                                                                                                    gTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGattatt                                                                                                                                              >  2:19551/1‑251 (MQ=255)                                                                                                        tGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGCATCGTCCCCGGACTGCCGCCAAATTGTTTCGCCAGTTAGGCGTAAGCGGTGCGCGCATTGCCCATTATTGCTTCCAGGATGCCACGGTCCAGATTGTCGGTCAAA‑TAATTTTCCATAGGATATTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTATTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGCATGATTATTGCAt                                                                                                                                          >  2:28459/1‑251 (MQ=255)                                                                                                                    gCTTCATTTTCGCTACTCGAACGTGTAGCTTCCCCGGACTGACGCCAAATTGTTTCGCCAGTGCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATGATTGCATGTGTGTCGGt                                                                                                                                <  2:228466/249‑1 (MQ=255)                                                                                                                                                         aCTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGtat                                                                                         <  1:47594/251‑1 (MQ=255)                                                                                                                                                                      tGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGAc                                                                                                   >  2:162235/1‑228 (MQ=255)                                                                                                                                                                      tGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGAc                                                                                                   <  1:162235/228‑1 (MQ=255)                                                                                                                                                                         gtCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGAGTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCAGGTGTGTCGGTGTGTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGc                                                                         <  2:310759/250‑1 (MQ=255)                                                                                                                                                                              cAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTAc                                                                     >  2:55184/1‑250 (MQ=255)                                                                                                                                                                                                            cATTAATGCTTCCAGGATGCAACGGTACAGATAGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTAGTg                                       >  1:279518/1‑250 (MQ=255)                                                                                                                                                                                                             aTTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGaa                                     <  1:212020/251‑1 (MQ=255)                                                                                                                                                                                                                      tCCAGGATGCCACGGTCCAGATTGTCGATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACt                             <  2:49321/250‑1 (MQ=255)                                                                                                                                                                                                                                                gATCAAA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTgg  >  1:120190/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       AGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGA‑TAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGG  >  NC_000913/3926747‑3927236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑