breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 57,276 IS2 (+) +5 bp intergenic (‑167/‑84) lptD ← / → djlA lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
RA 71,134 G→A G250S (GGT→AGT)  araC → DNA‑binding transcriptional dual regulator AraC
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
JC JC 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE
RA 612,046 G→A P150L (CCG→CTG)  fepA ← ferric enterobactin outer membrane transporter
RA 621,783 G→A A136V (GCC→GTC)  fepD ← ferric enterobactin ABC transporter membrane subunit FebD
RA 631,633 G→A W580* (TGG→TGA cstA → pyruvate transporter CstA
RA 642,784 G→A A233T (GCG→ACG)  ybdR → putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR
RA 643,506 G→A intergenic (‑87/+51) yldA ← / ← rnk protein YldA/nucleoside diphosphate kinase regulator
RA 646,861 G→A L217F (CTC→TTC)  citG ← triphosphoribosyl‑dephospho‑CoA synthase
RA 657,095 C→T A180V (GCC→GTC)  pagP → Lipid A palmitoyltransferase
RA 667,992 C→T G76S (GGT→AGT)  mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 949,985 G→A W106* (TGG→TGA ycaK → putative oxidoreductase YcaK
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,196,325 A→G K401K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,290,881 C→T L214F (CTT→TTT)  rssB → regulator of RpoS
MC JC 1,294,035 Δ141 bp [tdk] [tdk]
MC JC 1,299,499 Δ1,199 bp insH21 insH21
JC 1,303,073 Δ12 bp coding (175‑186/921 nt) oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,353,673 G→A L282L (CTC→CTT sapC ← putrescine ABC exporter membrane protein SapC
RA 1,353,758 T→A D254V (GAT→GTT)  sapC ← putrescine ABC exporter membrane protein SapC
RA 1,478,295 C→T L24L (CTA→TTA)  ynbB → putative CDP‑diglyceride synthase
RA 1,494,388 Δ1 bp coding (684/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,515,342 G→A G194E (GGA→GAA)  ydcV → putative ABC transporter membrane subunit YdcV
RA 1,517,508 G→T V40V (GTG→GTT ortT → orphan toxin OrtT
RA 1,521,520 G→A L516F (CTC→TTC)  yncD ← putative TonB‑dependent outer membrane receptor
RA 1,592,893 G→A intergenic (‑451/+5724) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,631,845 G→A A147A (GCC→GCT ydfJ ← putative transporter YdfJ
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,748,093 C→T R321R (CGC→CGT ydhS → FAD/NAD(P) binding domain‑containing protein YdhS
RA 1,749,123 C→T G146D (GGT→GAT)  ydhT ← uncharacterized protein YdhT
RA 1,750,502 C→T C171Y (TGC→TAC)  ydhX ← putative 4Fe‑4S ferredoxin‑like protein YdhX
RA 1,750,572 C→T A148T (GCA→ACA)  ydhX ← putative 4Fe‑4S ferredoxin‑like protein YdhX
RA 1,752,116 C→T R572H (CGT→CAT)  ydhV ← putative oxidoreductase YdhV
RA 1,757,109 A→G *471* (TAA→TGA)  pykF → pyruvate kinase 1
RA 1,772,389 C→T intergenic (+104/‑123) ydiM → / → ydiN putative exporter YdiM/putative transporter YdiN
RA 1,776,234 C→T H216H (CAC→CAT ydiF → putative acetate‑CoA transferase YdiF
RA 1,781,148 C→T L250L (CTG→TTG)  ydiR → putative electron transfer flavoprotein subunit YdiR
RA 1,789,643 C→T P11S (CCT→TCT)  ydiE → PF10636 family protein YdiE
JC JC 1,792,772 IS2 (–) +5 bp intergenic (‑41/+33) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
RA 1,794,304 C→T G283G (GGG→GGA btuC ← vitamin B12 ABC transporter membrane subunit
RA 1,802,144 C→T E143K (GAA→AAA)  thrS ← threonine‑‑tRNA ligase
RA 1,807,825 C→T S10S (AGC→AGT yniA → putative kinase YniA
RA 1,810,624 C→T I138I (ATC→ATT ydjM → inner membrane protein YdjM
RA 1,816,002 C→T D712D (GAC→GAT katE → catalase HPII
RA 1,820,949 C→T G89E (GGA→GAA)  chbC ← N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
RA 1,823,332 C→T intergenic (+47/‑183) nadE → / → cho NH3‑dependent NAD(+) synthetase/excinuclease Cho
RA 1,828,824 C→T R304Q (CGA→CAA)  astD ← aldehyde dehydrogenase
RA 1,831,373 C→T A204T (GCG→ACG)  astC ← succinylornithine transaminase
RA 1,831,664 C→T E107K (GAA→AAA)  astC ← succinylornithine transaminase
RA 1,833,493 C→T T31T (ACC→ACT ydjX → DedA family protein YdjX
RA 1,842,475 C→T L35L (CTC→CTT gdhA → glutamate dehydrogenase
RA 1,843,339 C→T T323T (ACC→ACT gdhA → glutamate dehydrogenase
RA 1,844,565 C→T A103T (GCT→ACT)  ynjI ← DUF1266 domain‑containing protein YnjI
RA 1,845,513 C→T R483Q (CGG→CAG)  topB ← DNA topoisomerase III
RA 1,850,545 C→T A570V (GCA→GTA)  sppA → protease IV, a signal peptide peptidase
RA 1,851,614 C→T A252V (GCC→GTC)  ansA → L‑asparaginase 1
RA 1,855,005 C→T A323T (GCC→ACC)  ydjG ← NADH‑dependent methylglyoxal reductase
RA 1,857,164 C→T P202P (CCG→CCA ydjI ← L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase
RA 1,858,800 C→T G12S (GGC→AGC)  ydjJ ← putative zinc‑binding dehydrogenase YdjJ
RA 1,866,710 C→T intergenic (‑238/‑198) mipA ← / → yeaG MltA‑interacting protein/protein kinase YeaG
RA 1,871,516 C→T R378C (CGT→TGT)  cdgI → putative c‑di‑GMP binding protein CdgI
RA 1,914,009 C→T A270T (GCA→ACA)  prc ← tail‑specific protease
RA 1,916,210 C→T intergenic (‑82/‑48) msrC ← / → letA free methionine‑(R)‑sulfoxide reductase/intermembrane transport protein LetA
RA 1,950,945 G→A G38G (GGG→GGA yecD → isochorismatase family protein YecD
RA 1,951,285 G→A A152T (GCC→ACC)  yecD → isochorismatase family protein YecD
RA 1,951,576 G→A C61Y (TGT→TAT)  yecE → DUF72 domain‑containing protein YecE
RA 1,953,546 G→A Q35Q (CAG→CAA cmoB → tRNA U34 carboxymethyltransferase
RA 1,978,243 G→T intergenic (‑46/+275) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 1,982,189 G→A L67F (CTT→TTT)  otsB ← trehalose‑6‑phosphate phosphatase
RA 1,983,899 G→A P391S (CCG→TCG)  araG ← arabinose ABC transporter ATP binding subunit
RA 2,003,264 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,242,121 A→G S283P (TCG→CCG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,259,422 A→C intergenic (‑126/+297) psuK ← / ← fruA putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA
JC JC 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
JC JC 2,319,850 IS2 (+) +5 bp coding (23‑27/2850 nt) rcsC ← histidine kinase RcsC
RA 2,397,444 G→A Q908* (CAA→TAA)  nuoG ← NADH:quinone oxidoreductase subunit G
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
JC JC 2,666,074 IS1 (+) +9 bp coding (640‑648/1281 nt) csiE → stationary phase‑inducible protein CsiE
RA 3,224,628 C→T L666L (CTG→TTG)  ebgA → evolved beta‑D‑galactosidase subunit alpha
RA 3,268,970 A→G T186A (ACA→GCA)  yhaC → uncharacterized protein YhaC
RA 3,319,705 A→G T357A (ACT→GCT)  argG → argininosuccinate synthetase
RA 3,320,867 C→T P249P (CCG→CCA yhbX ← putative hydrolase YhbX
RA 3,323,477 C→T G198G (GGG→GGA glmM ← phosphoglucosamine mutase
RA 3,331,507 C→T G83D (GGT→GAT)  obgE ← GTPase ObgE
RA 3,337,144 C→T G38E (GGG→GAG)  mlaB ← intermembrane phospholipid transport system protein MlaB
RA 3,346,861 C→T A95V (GCT→GTT)  ptsN → phosphotransferase system enzyme IIA(Ntr)
RA 3,350,292 C→T A56A (GCG→GCA elbB ← low activity glyoxalase ElbB
RA 3,360,240 C→T P348L (CCG→CTG)  gltD → glutamate synthase subunit GltD
RA 3,360,951 C→T intergenic (+335/‑225) gltD → / → gltF glutamate synthase subunit GltD/periplasmic protein GltF
RA 3,361,564 C→T A130V (GCC→GTC)  gltF → periplasmic protein GltF
RA 3,362,459 C→T S116S (AGC→AGT yhcA → putative fimbrial chaperone YhcA
RA 3,363,653 C→T L283L (CTG→TTG)  yhcD → putative fimbrial usher protein YhcD
RA 3,383,369 C→T K300K (AAG→AAA mdh ← malate dehydrogenase
RA 3,397,982 C→T D133N (GAC→AAC)  yhdE ← nucleoside triphosphate pyrophosphatase YhdE
RA 3,406,476 C→T A187V (GCG→GTG)  accC → biotin carboxylase
RA 3,432,173 G→A L98F (CTC→TTC)  smg ← DUF494 domain‑containing protein Smg
RA 3,439,614 G→A intergenic (‑105/+2) yhdN ← / ← rplQ DUF1992 domain‑containing protein YhdN/50S ribosomal subunit protein L17
RA 3,445,876 G→A L89L (CTG→TTG)  rplF ← 50S ribosomal subunit protein L6
RA 3,446,190 G→A A119V (GCT→GTT)  rpsH ← 30S ribosomal subunit protein S8
RA 3,449,865 G→A P7S (CCT→TCT)  rpsC ← 30S ribosomal subunit protein S3
RA 3,454,722 T→C Y226C (TAC→TGC)  gspA ← Type II secretion system protein GspA
RA 3,459,326 G→A V330I (GTT→ATT)  gspE → Type II secretion system protein GspE
RA 3,469,561 G→A S98F (TCT→TTT)  chiA ← endochitinase
RA 3,469,763 G→A Q31* (CAA→TAA)  chiA ← endochitinase
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,496,485 G→A E825E (GAG→GAA nirB → nitrite reductase (NADH) large subunit
RA 3,506,702 G→A A212V (GCC→GTC)  yhfT ← uncharacterized protein YhfT
RA 3,511,870 G→A L159L (CTG→TTG)  yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,514,651 G→A L137L (CTG→TTG)  rpe ← ribulose‑phosphate 3‑epimerase
RA 3,541,403 G→A D333N (GAC→AAC)  feoB → Fe(2(+)) transporter FeoB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,564,733 G→A Q617* (CAG→TAG)  glgP ← glycogen phosphorylase
RA 3,573,001 G→A L168L (CTG→TTG)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,575,597 G→A G176E (GGA→GAA)  yhgN → MarC family putative inner membrane protein YhgN
RA 3,587,922 G→A G64G (GGC→GGT ugpQ ← glycerophosphodiester phosphodiesterase UgpQ
RA 3,587,957 G→A L53F (CTC→TTC)  ugpQ ← glycerophosphodiester phosphodiesterase UgpQ
RA 3,595,740 G→A L222L (CTG→TTG)  livH ← branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH
RA 3,602,478 T→C L90L (CTA→CTG ftsE ← cell division protein FtsE
RA 3,603,367 G→A P293S (CCG→TCG)  ftsY ← signal recognition particle receptor
RA 3,647,997 G→A intergenic (‑209/‑66) dinQ ← / → agrA membrane toxin DinQ/small RNA AgrA
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,786,064 G→A E269K (GAA→AAA)  gpmM → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
RA 3,788,378 C→T T93M (ACG→ATG)  yibQ → divergent polysaccharide deacetylase domain‑containing protein YibQ
RA 3,790,380 C→T Q322Q (CAG→CAA tdh ← threonine dehydrogenase
RA 3,796,150 G→A A58T (GCA→ACA)  waaC → ADP‑heptose:LPS heptosyltransferase 1
RA 3,802,483 G→A A192A (GCC→GCT waaO ← UDP‑D‑glucose:(glucosyl)LPS alpha‑1,3‑glucosyltransferase
RA 3,830,893 A→G N146S (AAT→AGT)  yicH → AsmA family protein YicH
RA 3,841,368 C→T G124G (GGG→GGA nepI ← purine ribonucleoside exporter
RA 3,841,478 C→T V88I (GTT→ATT)  nepI ← purine ribonucleoside exporter
RA 3,852,000 C→T G262E (GGG→GAG)  ilvB ← acetohydroxy acid synthase I subunit IlvB
RA 3,868,421 C→T V297I (GTC→ATC)  yidR ← DUF3748 domain‑containing galacturonate catabolism protein YidR
RA 3,871,577 C→T E52K (GAA→AAA)  dgoT ← D‑galactonate:H(+) symporter
RA 3,873,336 C→T V93M (GTG→ATG)  dgoA ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA 3,880,045 C→T M25I (ATG→ATA gyrB ← DNA gyrase subunit B
RA 3,886,749 G→A intergenic (+27/‑79) yidC → / → mnmE membrane protein insertase YidC/5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
RA 3,891,231 G→A L332L (CTG→CTA tnaB → tryptophan:H(+) symporter TnaB
RA 3,891,677 G→A M21I (ATG→ATA mdtL → efflux pump MdtL
RA 3,892,396 C→T A261V (GCG→GTG)  mdtL → efflux pump MdtL
RA 3,895,645 C→T G322D (GGC→GAC)  adeP ← adenine:H(+) symporter
RA 3,896,116 C→T G165D (GGT→GAT)  adeP ← adenine:H(+) symporter
RA 3,898,302 G→A G94D (GGT→GAT)  cbrC → PF03691 family colicin E2 tolerance protein CbrC
RA 3,949,826 G→A R48R (CGC→CGT yifB ← putative magnesium chelatase YifB
RA 3,953,554 G→A G26E (GGA→GAA)  ilvD → dihydroxy‑acid dehydratase
RA 3,956,739 C→T T470I (ACC→ATC)  ilvA → threonine deaminase
RA 3,957,961 G→A intergenic (‑141/‑9) ilvY ← / → ilvC DNA‑binding transcriptional dual regulator IlvY/ketol‑acid reductoisomerase (NADP(+))
RA 3,960,596 G→A intergenic (‑783/‑81) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 4,027,754 G→A A340V (GCG→GTG)  fadA ← 3‑ketoacyl‑CoA thiolase
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
JC JC 4,135,180 IS2 (–) +5 bp coding (1346‑1350/2181 nt) katG → catalase/hydroperoxidase HPI
RA 4,146,389 (T)6→7 coding (132/879 nt) pflC → putative pyruvate formate‑lyase 2 activating enzyme PflC
RA 4,161,093 C→T S9S (AGC→AGT fabR → DNA‑binding transcriptional repressor FabR
RA 4,206,024 G→A I503I (ATC→ATT purH ← bifunctional AICAR transformylase/IMP cyclohydrolase
RA 4,207,461 G→A A24A (GCC→GCT purH ← bifunctional AICAR transformylase/IMP cyclohydrolase
RA 4,209,562 G→A noncoding (1416/1542 nt) rrsE → 16S ribosomal RNA
RA 4,236,742 C→T P161S (CCG→TCG)  yjbF → lipoprotein YjbF
RA 4,246,296 C→T G42S (GGC→AGC)  malE ← maltose ABC transporter periplasmic binding protein
RA 4,281,878 C→T D400N (GAT→AAT)  yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,352,327 A→G K81K (AAA→AAG yjdJ → putative N‑acetyltransferase YjdJ
RA 4,371,696 C→T D224D (GAC→GAT groL → chaperonin GroEL
RA 4,429,371 C→T P146L (CCC→CTC)  fklB → peptidyl‑prolyl cis‑trans isomerase FklB
JC JC 4,536,236 IS5 (+) +4 bp intergenic (‑2183/‑358) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,537,366 C→T G77S (GGT→AGT)  nanS ← N‑acetyl‑9‑O‑acetylneuraminate esterase
RA 4,542,683 Δ1 bp intergenic (+50/‑432) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196347 1211482 15136 5 [4] [4] 5 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270 776 22 [0] [0] 20 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423873–3424232 3424435–3424238 7–563 5 [4] [4] 5 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)43 (1.060) 41/490 0.2 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 382183 NA (NA)noncoding (924/1331 nt) IS2 repeat region
* ? NC_000913 382179 =NA (NA)37 (0.920) 37/486 0.2 NA noncoding (920/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 56677438 (0.930)13 (0.320) 8/486 2.4 26.9% pseudogene (91/93 nt) renD protein RenD
?NC_000913 568036 = 33 (0.820)pseudogene (2/213 nt) renD protein RenD
* ? NC_000913 = 1397238NA (NA)22 (0.540) 21/490 0.9 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)20 (0.490) 19/490 1.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL