Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,444	G→A	Q908* (CAA→TAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,444	0	G	A	100.0%	173.6 / NA	52	Q908* (CAA→TAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/21);  total (31/21)

TGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGC  >  NC_000913/2397199‑2397684                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 tGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATtgtg                                                                                                                                                                                                                                             >  1:259909/1‑251 (MQ=255)    cgcgAGCCGAGAATTTCGGGATCCAGTCTTCTTAAAAGAACATTTTGATCATTACCGCACCAAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGcct                                                                                                                                                                                                                                          <  1:313162/251‑1 (MQ=255)     gcgATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGcctc                                                                                                                                                                                                                                         <  1:187697/251‑1 (MQ=255)                                  cttTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCgccagcc                                                                                                                                                                                                             >  1:386328/1‑250 (MQ=255)                                       aaGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGCTGGTCAGCAGGATCTCAATCATTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGATATCCTCAATCTGCGCGCCATCCAGCAcc                                                                                                                                                                                                       >  1:46544/1‑251 (MQ=255)                                            cATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGc                                                                                                                                                                                                  >  2:48945/1‑251 (MQ=255)                                             aTTTTGCTCATTTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCa                                                                                                                                                                                                 <  1:286141/251‑1 (MQ=255)                                                ttGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTATGGAACAGACCCAGCAGGCGACGTTCGGCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTAATGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATg                                                                                                                                                                                              >  1:48579/1‑251 (MQ=255)                                                   aTCATGTCCGCAACCAGCTGGAGCGAACCGACCCAGCCAACACGGTTAGGTCCGTAACGGTTATGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGAATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCg                                                                                                                                                                                           >  1:400709/1‑251 (MQ=255)                                                           cGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATAcc                                                                                                                                                                                   >  1:416053/1‑251 (MQ=255)                                                                ccAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCg                                                                                                                                                                              <  1:108922/251‑1 (MQ=255)                                                                     ggaagggAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAg                                                                                                                                                                         <  2:408767/245‑1 (MQ=255)                                                                        aGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGccc                                                                                                                                                                      >  2:117064/1‑251 (MQ=255)                                                                          ggAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAc                                                                                                                                                                    <  2:386880/250‑1 (MQ=255)                                                                          cGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCACCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAAATCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGTACATACCCATCGGCAAGCCCAc                                                                                                                                                                    >  1:71593/1‑251 (MQ=255)                                                                                  cccAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctgccctg                                                                                                                                                            >  1:64188/1‑251 (MQ=255)                                                                                    cAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACctgccctgcc                                                                                                                                                          <  1:43188/251‑1 (MQ=255)                                                                                      gCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGt                                                                                                                                                        >  1:136332/1‑251 (MQ=255)                                                                                         aaCACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTc                                                                                                                                                       >  1:137279/1‑249 (MQ=255)                                                                                              ggTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGATCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCtt                                                                                                                                                >  2:172175/1‑251 (MQ=255)                                                                                                   ggTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCg                                                                                                                                           <  1:19175/251‑1 (MQ=255)                                                                                                       cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGAttt                                                                                                                                       <  2:137279/251‑1 (MQ=255)                                                                                                             gggtCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCGCCAGCAGGATCACCACCGCGTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATGGGGCCTCCTTGAGATCCTCAAGATGCTCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCg                                                                                                                                 <  1:196795/248‑1 (MQ=255)                                                                                                                   agAACAGCCGCAGCAGGCGACGTCGGCGCAAGCTCTGGAATGCCCCGAAGGTGTCACACACCAGCAGGATCACCACCGCTTTGCGGATCGTCAGCAGGAGGTCAATCAGTTACGGTGATATGCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGAAGCGCGCCAGCCAGAACCGGAGCAATGCCGGACATACCTATCGACAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCg                                                                                                                           >  2:20329/2‑251 (MQ=255)                                                                                                                    gAACAGACCTAGCAGGCGACTTTCACCAAAGCTCATGAATGCCGCGCAGGTGATAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAAGCAGGTCCGGGGATAGCCAACGCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCCCCGGAACAATGCCGGACATACTCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGt                                                                                                                          <  2:71593/251‑1 (MQ=255)                                                                                                                                     gACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGt                                                                                                         >  1:395982/1‑251 (MQ=255)                                                                                                                                      aCGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGACCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGt                                                                                                         >  2:232566/1‑250 (MQ=255)                                                                                                                                        gTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAAc                                                                                                      <  1:232566/251‑1 (MQ=255)                                                                                                                                        gTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGNAAc                                                                                                      >  2:368383/1‑251 (MQ=255)                                                                                                                                           cGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTa                                                                                                    >  1:201338/1‑250 (MQ=255)                                                                                                                                             ccAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGCGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTaaac                                                                                                 >  2:331178/1‑250 (MQ=255)                                                                                                                                               aaaGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGTCAAGCCCACCTGCCCTGCCGTCAATCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGga                                                                                               <  1:6671/251‑1 (MQ=255)                                                                                                                                               aaaGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGga                                                                                               <  1:345472/251‑1 (MQ=255)                                                                                                                                                      aTGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGCGACGCgt                                                                                        >  1:376200/1‑251 (MQ=255)                                                                                                                                                        gAATGCCCCGGAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTGGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCGGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCgtgt                                                                                      <  1:72677/251‑1 (MQ=255)                                                                                                                                                             ccccGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTg                                                                                 >  1:76306/1‑251 (MQ=255)                                                                                                                                                                  cAGGTGACAAGCACCAGAAGCATCTCCACCGCTTTGAGTAGGGTCATAAGGATCTCAATCAGTACCGGTGATATCCACCTCATTATTGTGCCCCCTTGAGATCCTCAAGATGCGCGCCAGCGAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTGCAACCGGCAGCGTGACCGTGTTGCTATCGTAACTAAAGGAGACGCGTGTCCCTGCTTTc                                                                            <  2:145399/251‑1 (MQ=255)                                                                                                                                                                   aGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTc                                                                            >  1:59634/1‑250 (MQ=255)                                                                                                                                                                                  gcagGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCgg                                                            <  1:415706/251‑1 (MQ=255)                                                                                                                                                                                     ggATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCAt                                                         <  1:11914/251‑1 (MQ=255)                                                                                                                                                                                      gATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATc                                                        >  1:346109/1‑251 (MQ=255)                                                                                                                                                                                              ccGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGt                                                <  1:185458/251‑1 (MQ=255)                                                                                                                                                                                                gCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGGGTTCACACCCAACTTCGCGGCATCCGCTGGGTTg                                              >  1:285891/1‑251 (MQ=255)                                                                                                                                                                                                  tttGAGGCTGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCAAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTg                                              >  1:342975/1‑249 (MQ=255)                                                                                                                                                                                                     gagtctagTCAGCATGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCCGCAGCGTGACCGTGTTTCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTCCACACCCAACTTCGCGGCATCCGCTGGGTTGAGtt                                          <  2:29558/243‑1 (MQ=255)                                                                                                                                                                                                                      tctcAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGc                         >  1:139112/1‑250 (MQ=255)                                                                                                                                                                                                                          aaTCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACg                    >  1:348876/1‑251 (MQ=255)                                                                                                                                                                                                                              aGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGctc                >  2:279703/1‑251 (MQ=255)                                                                                                                                                                                                                               gTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGctct               >  2:121664/1‑251 (MQ=255)                                                                                                                                                                                                                                          atCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGCCCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCgg     >  2:24464/1‑250 (MQ=255)                                                                                                                                                                                                                                          atCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCTGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCgg     >  1:163453/1‑250 (MQ=255)                                                                                                                                                                                                                                            ccAACGCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGGCCGGAGc  <  1:349184/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 TGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGAGC  >  NC_000913/2397199‑2397684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑