Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,572	C→T	A148T (GCA→ACA)	ydhX ←	putative 4Fe‑4S ferredoxin‑like protein YdhX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,572	0	C	T	100.0%	136.9 / NA	40	A148T (GCA→ACA)	ydhX	putative 4Fe‑4S ferredoxin‑like protein YdhX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/14);  total (26/14)

TGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGC  >  NC_000913/1750335‑1750814                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   tGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGAc                                                                                                                                                                                                                                        >  1:319657/1‑250 (MQ=255)                            tAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACgg                                                                                                                                                                                                             <  2:90378/250‑1 (MQ=255)                                cAAATGTTGACCGAACCGGCGATACAGATGCGGTTGCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACTCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTTTTACGGACaa                                                                                                                                                                                                         <  2:163460/250‑1 (MQ=255)                                cAAATGTTGACCGAACCGGCGATACAGATACGGTTTCCCGGCACCTGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACACCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCCGCCGGGACTCAGCGCAGCAATCACATTTATCTGTCACTTTAGTGACTGGGTTAAGGTATCGAATCTGGTACGGACaac                                                                                                                                                                                                        >  2:341869/1‑250 (MQ=255)                                  aatgtctaCCGATCCGGCGATACAGATGCGGTGTTCCGTCACCAGGTAGCTGATATTGATAGTATTTATGTTGCTGCAACCACTCCTGGATCTCTGGGCTATCTTCTCGACCAAAGATTAATGCGTGTTCCGGGTGGGCGCTGACGCAAATGGGCGGAAGGCCTTTTGCCAGTCGGGACTCAGCGCATAAATCACATTTTTCTATCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAgcg                                                                                                                                                                                                      <  1:34110/244‑1 (MQ=255)                                  aaTGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAgcg                                                                                                                                                                                                      >  2:7804/1‑251 (MQ=255)                                      ttGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAgc                                                                                                                                                                                                       <  2:393137/246‑1 (MQ=255)                                      ttGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAgc                                                                                                                                                                                                       >  1:393137/1‑246 (MQ=255)                                                cGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACt                                                                                                                                                                                        >  1:203145/1‑251 (MQ=255)                                                 ggCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGTACAAGCGCCAATGCAATAACTg                                                                                                                                                                                       <  1:90671/251‑1 (MQ=255)                                                          gATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGa                                                                                                                                                                                >  2:119647/1‑249 (MQ=255)                                                                  ttCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCg                                                                                                                                                                       <  1:28987/250‑1 (MQ=255)                                                                               gTAGCTGATATTGGTAGTATTTATTTTGCTGCAACCAAGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGc                                                                                                                                                         >  2:296959/1‑251 (MQ=255)                                                                                              aGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCtc                                                                                                                                           >  2:152510/1‑250 (MQ=255)                                                                                                     atttTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGcc                                                                                                                                   >  1:34088/1‑251 (MQ=255)                                                                                                              cAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGccc                                                                                                                          >  1:69461/1‑251 (MQ=255)                                                                                                                  cACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGt                                                                                                                      >  1:253413/1‑251 (MQ=255)                                                                                                                  cACGCCTGAATTTCTGGGCTATCATCGCGACCAAAGATCGATACGAGTTCCGGGTAGGCACTGACGCAAATGGGCGGAAAGCCTTTTGCAAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGAAAGGGTTAAGGAAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCAATGCGATTTTTCTACCCGCTCAATCCCCTGCTCATCACGCAACGACGCCCCCGt                                                                                                                      >  2:222644/1‑251 (MQ=255)                                                                                                                         gAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCa                                                                                                                >  2:310623/1‑250 (MQ=255)                                                                                                                           aTTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAAc                                                                                                             >  1:133715/1‑251 (MQ=255)                                                                                                                             ttCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGt                                                                                                           >  1:183752/1‑251 (MQ=255)                                                                                                                                          tCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCGTCACGCCACGACGCCCCCGTCGGGCAATCGTCGATGCACGGa                                                                                                <  2:242500/249‑1 (MQ=255)                                                                                                                                               aCCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGCTTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCtt                                                                                         >  2:170782/1‑251 (MQ=255)                                                                                                                                                      aTCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTg                                                                                  <  1:310623/251‑1 (MQ=255)                                                                                                                                                        cAATTCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGCGTCTCAGCGCAGACATCAAATTTATCTGTCAGTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGGGCAAATGCAATAACTGCAGCCGAGGCACTGCGATTTTTCTACCCGAACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGATCATCGTCGCACTGCt                                                                                >  2:207669/1‑251 (MQ=255)                                                                                                                                                                gtTCCGGGTAGGCGCTGAAGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATGTGTCACTTAAGTGACAGCGTTAAGGTAGCGGACCTGGTACGGACAAGAGCCAATGCAATAACAGCAGCCGATGCACTGTGATACTGCTAACCGCACCATCCCCTGCTCATCACGCCACGACGCCCCCTTCGCGCAAACGTCGATGCACGGCTCATCTTCACAGTGCTGACATGAc                                                                        >  2:155188/1‑251 (MQ=255)                                                                                                                                                                   ccGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTgg                                                                     >  1:348969/1‑251 (MQ=255)                                                                                                                                                                   ccGGGTAGGCGCCGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGTAGCATCTTCGCAGTGCTGACATGACTg                                                                      <  1:152510/250‑1 (MQ=255)                                                                                                                                                                                        gctcagggaagacTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGtt                                                >  2:336667/13‑251 (MQ=255)                                                                                                                                                                                         gggCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGttt                                               >  1:321557/1‑251 (MQ=255)                                                                                                                                                                                              gAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTg                                          <  1:403082/251‑1 (MQ=255)                                                                                                                                                                                                   ccTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGtt                                     <  2:90189/251‑1 (MQ=255)                                                                                                                                                                                                         gCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGt                               <  1:52043/251‑1 (MQ=255)                                                                                                                                                                                                                        gcgcAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGc                <  2:319657/251‑1 (MQ=255)                                                                                                                                                                                                                         cgcAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCt               >  1:293691/1‑251 (MQ=255)                                                                                                                                                                                                                             gAAATCACATTTATCTGTAACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCACCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGATAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTNTCg           >  2:381614/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaTCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGCGCATCTTCGCAGTGCTGACATGACTGGCGAAACAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCg           >  2:401070/1‑250 (MQ=255)                                                                                                                                                                                                                                aTCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCAGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGAGCGGACATGAATGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGGGCTATCGAt         >  1:313449/1‑250 (MQ=255)                                                                                                                                                                                                                                   acaTTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAAc     >  2:280974/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAAGGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCTTCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGc  <  2:24173/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   TGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGC  >  NC_000913/1750335‑1750814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑