Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,631,845	G→A	A147A (GCC→GCT)	ydfJ ←	putative transporter YdfJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,631,845	0	G	A	100.0%	86.8 / NA	26	A147A (GCC→GCT)	ydfJ	putative transporter YdfJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/10);  total (16/10)

TACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCACCAGCAAGATGGGTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGA  >  NC_000913/1631604‑1632089                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     aaaccGCCTGAACAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGAtt                                                                                                                                                                                                                                             <  2:312864/248‑1 (MQ=255)       ccTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGtt                                                                                                                                                                                                                                       >  2:196842/1‑251 (MQ=255)                   gCAGCCCTTTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAgctt                                                                                                                                                                                                                           <  2:138839/251‑4 (MQ=255)                       cccTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGc                                                                                                                                                                                                                       >  2:11835/1‑251 (MQ=255)                                   ccagAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCa                                                                                                                                                                                                           <  1:11835/251‑1 (MQ=255)                                   ccagAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACACCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTccc                                                                                                                                                                                                            >  2:49481/1‑250 (MQ=255)                                    cagAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCAt                                                                                                                                                                                                          >  2:156885/1‑251 (MQ=255)                                              tGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAAt                                                                                                                                                                                                >  1:46675/1‑251 (MQ=255)                                                        cATGCTACCAGCAGGTGCAGGTTTTGATGTCGGGTTGTTACTGTCGTTAACCTTCTCGAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCCTCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGACCAGATTGCCGTTGCGCTCAAGGTTCCGCAGGTAGTTCCCATAGCCACAAATGAGGAGATa                                                                                                                                                                                       >  2:35872/1‑250 (MQ=255)                                                          tGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAAtt                                                                                                                                                                                    >  1:113694/1‑251 (MQ=255)                                                                cagcagTTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCgcgc                                                                                                                                                                              <  1:36018/251‑1 (MQ=255)                                                                            gtTTTGCTGTCTGTTGGTTACTGTCGTTAACCTTCACAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTGCCGTTTGgc                                                                                                                                                                  <  2:183484/251‑1 (MQ=255)                                                                                  cTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGCTTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGACGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTTGCGCatat                                                                                                                                                            >  1:18398/1‑251 (MQ=255)                                                                                    gTCGGTTGGTTACTGTCGTTAACCTACTCAAAGACCGGGCTTGCTTTCAGATTCATACGCAACCAGATAGCAAATACCATCTCGACTACACTCGCCAGGAACGGTAAACGCCATCCCCGCGCCAGCATTTCCTCTTTACTGAGAGTTAAGAACATAAAAGCCCAGATGGCCGTTGCGCTCAAGTTTCCGCAGTTAGTGCCCATATCCGCAAATGAGGAGATATTTCCGCCCTTTCCTTTTGGCGCatat                                                                                                                                                            <  2:265753/249‑1 (MQ=255)                                                                                                         aCCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCAcc                                                                                                                                     <  1:374864/251‑1 (MQ=255)                                                                                                                 aaaGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGTCACCGGAAAttt                                                                                                                             >  2:349104/1‑251 (MQ=255)                                                                                                                    gACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCg                                                                                                                          >  1:348640/1‑251 (MQ=255)                                                                                                                              tctttcAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCAcc                                                                                                                >  1:270413/1‑251 (MQ=255)                                                                                                                                                               aCCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCCGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCACCAGCa                                                                                >  1:224848/1‑250 (MQ=255)                                                                                                                                                                    cACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCACCAGCAAGATgg                                                                          >  1:353099/1‑251 (MQ=255)                                                                                                                                                                                                cGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAAGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGTATAATACGCAACGTCACCAGCAAGATGGGTGCAAAAACACCAATCTGTGCATAGGt                                              >  1:374801/1‑251 (MQ=255)                                                                                                                                                                                                                                gagaATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCACCAGCAAGATGGGTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATcc              <  1:163343/251‑1 (MQ=255)                                                                                                                                                                                                                                  gaATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCACCAGCAAGATGGGTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAggtg                        <  1:355492/239‑1 (MQ=255)                                                                                                                                                                                                                                  gaATGAAGAACATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCACCAGCAAGATGGGTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAggtg                        >  2:355492/1‑239 (MQ=255)                                                                                                                                                                                                                                       aagaaCATAAAAGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCACCAGCAAGATGGGTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCcatcat       >  1:328248/1‑251 (MQ=255)                                                                                                                                                                                                                                            aaTAAAAGCCCAGATTGCCGTTGAGCTTAAGGTTCCGCATTTAGTTCCCATAGCCACAAATGAGGAGATAAGTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCTCCAGCAAGATGGGTGCAAAAACACCAATCTGTGCATAGTTCGGTAACTCACCAATTAAGGTGGTACAGATCCCCATCATGGTGa  <  2:224848/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     TACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCATATTCCGCCAGCATCGTACCGGCACCGGAAATTTCCGCACCTGCACCCAACCCCTGAATAATACGCAACGTCACCAGCAAGATGGGTGCAAAAACACCAATCTGTGCATAGGTCGGTAACACACCAATTAAGGTGGTACAGATCCCCATCATGGTGA  >  NC_000913/1631604‑1632089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑