Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,842,475	C→T	L35L (CTC→CTT)	gdhA →	glutamate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,842,475	0	C	T	100.0%	117.4 / NA	34	L35L (CTC→CTT)	gdhA	glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

CGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAA  >  NC_000913/1842235‑1842718                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    cGTTATTAATTTGCGTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGAAGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAATCACAATCGGATTAATATATAAGGGTTGTATATCTATGGAGCAGACATATTCTCTGGATTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACTGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCtttt                                                                                                                                                                                                                                            <  2:293896/250‑1 (MQ=255)         aTTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACaa                                                                                                                                                                                                                                   <  2:37894/251‑1 (MQ=255)          tttGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGCATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGCAGTAATGACCACACTTTGGCCTTTTCTTGAACaa                                                                                                                                                                                                                                   >  1:91741/1‑250 (MQ=255)             gCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAAt                                                                                                                                                                                                                                <  1:285775/250‑1 (MQ=255)                                    tGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAATCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCAtt                                                                                                                                                                                                         <  1:175452/250‑1 (MQ=255)                                    tGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCAt                                                                                                                                                                                                          <  1:377368/249‑1 (MQ=255)                                         aCCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTgg                                                                                                                                                                                                    <  2:233704/250‑1 (MQ=255)                                                           gTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCgg                                                                                                                                                                                  >  2:309088/1‑250 (MQ=255)                                                                           cAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGttt                                                                                                                                                                 >  1:347605/1‑251 (MQ=255)                                                                                    aTGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTa                                                                                                                                                        <  1:126330/251‑1 (MQ=255)                                                                                      gACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATTGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTAt                                                                                                                                                       <  1:27894/250‑1 (MQ=255)                                                                                         aTAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGt                                                                                                                                                   <  1:140690/251‑1 (MQ=255)                                                                                              caacaTAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTtgatg                                                                                                                                              <  2:150019/251‑1 (MQ=255)                                                                                              caacaTAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGCGATCCAGTTTCGCGTGGTATGGGTtgatg                                                                                                                                              <  1:211887/251‑1 (MQ=255)                                                                                               aacaTAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTtgatga                                                                                                                                             <  1:137704/251‑1 (MQ=255)                                                                                                acaTAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTgatgat                                                                                                                                            >  1:287585/1‑251 (MQ=255)                                                                                                       cacaATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAACTCGACCCGAATCAAACCGATTTCGCGCATGCCGTTCGTGAAGTAATGACCACTCTTTGGCCGTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCTCGTGATCCAGTTTCGCGTGGTATTGGTTGATGATCGCAAc                                                                                                                                      <  1:239699/250‑1 (MQ=255)                                                                                                                 ttAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGt                                                                                                                           >  1:162749/1‑251 (MQ=255)                                                                                                                                  tataTCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATACAGTTTCGCGTGGTATGGGTTGATGATCGCACCCAGATACAGGTCAACCGTGCATGGCgtg                                                                                                          >  2:123659/1‑251 (MQ=255)                                                                                                                                    tatCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGtgtg                                                                                                        >  2:146257/1‑251 (MQ=255)                                                                                                                                           ggATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTc                                                                                                  >  2:290848/1‑250 (MQ=255)                                                                                                                                                  aCATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGAATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGc                                                                                          >  1:402494/1‑251 (MQ=255)                                                                                                                                                    atatTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCTAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGcc                                                                                         >  1:216115/1‑250 (MQ=255)                                                                                                                                                               aGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCa                                                                                                 <  2:417270/231‑1 (MQ=255)                                                                                                                                                               aGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCa                                                                                                 >  1:417270/1‑231 (MQ=255)                                                                                                                                                                      ccTCAACCATTGCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGc                                                                       <  2:287585/250‑1 (MQ=255)                                                                                                                                                                             cATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATgcg                                                               >  1:49185/1‑251 (MQ=255)                                                                                                                                                                              aTGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTGCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATgcgc                                                              <  2:364252/251‑1 (MQ=255)                                                                                                                                                                                                 aaaNCAAACCGAGTTCGCCCAAGCCGTTCGTGAATTAATTAACACACTTTGGCCTTTTCTTGAACAAAATCCAAAAGTTCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAAcc                                           <  2:380499/250‑1 (MQ=255)                                                                                                                                                                                                          cGAGTTCGCGCAAGCGGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGCAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCCTCGGCCCGTACAAAGGCGGTAAGCGCTTCCACCCCTCAGTTAACCTTTCCAttt                                  >  2:25978/1‑250 (MQ=255)                                                                                                                                                                                                              ttCGCGCAAGCCGTTCGTGAAGTAATGACCACACTTTGGCCTTTTCTTGAACACAATCCCAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCa                               >  1:411394/1‑250 (MQ=255)                                                                                                                                                                                                                                 aaGTAATGGCCACACTTTGGCCTTGTCTTGAACAAAATCCAAAATATCGCCAGATTGCATTACTGGAGCGTCTGGTTGAACCGGAGTGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCTGTATGCGCTTTCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCGCAGCGTTGAACaa           <  2:241169/251‑1 (MQ=255)                                                                                                                                                                                                                                           cacaCTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGCGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATGCGCTTACATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGCACAAACCTTCaaa  >  1:34687/1‑250 (MQ=255)                                                                                                                                                                                                                                           cacaCTTTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCaaa  >  1:124855/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    CGTTATTAATTTGCTTTCCTGGGTCATTTTTTTCTTGCTTACCGTCACATTCTTGATGGTATAGTCGAAAACTGCAAAAGCACATGACATAAACAACATAAGCACAATCGTATTAATATATAAGGGTTTTATATCTATGGATCAGACATATTCTCTGGAGTCATTCCTCAACCATGTCCAAAAGCGCGACCCGAATCAAACCGAGTTCGCGCAAGCCGTTCGTGAAGTAATGACCACACTCTGGCCTTTTCTTGAACAAAATCCAAAATATCGCCAGATGTCATTACTGGAGCGTCTGGTTGAACCGGAGCGCGTGATCCAGTTTCGCGTGGTATGGGTTGATGATCGCAACCAGATACAGGTCAACCGTGCATGGCGTGTGCAGTTCAGCTCTGCCATCGGCCCGTACAAAGGCGGTATGCGCTTCCATCCGTCAGTTAACCTTTCCATTCTCAAATTCCTCGGCTTTGAACAAACCTTCAAA  >  NC_000913/1842235‑1842718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑