Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,7510C.100.0% 74.2 / NA 18coding (1295/1374 nt)mdtKmultidrug efflux pump MdtK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (10/8);  total (10/8)

GAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCCCCTT  >  NC_000913/1744501‑1744992
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 
gagcctGTAACGCTGGATGCGCATTTGATGATGCTGGCGGCGGTATATAAGATTTCTGACTAAATTCAGGTGACTGGCAGTGGGATTTTGCGCGGTTCTAAAGATACGCGTGCCATTTTCTAGATTACCTTTTCGGCCTACTGGGTGATGGGCTTGCTAAGCGGATATATTATGGTAATGACCGATCTGGTCGTTGAACCTATGGGGCCTGCAGTCTTCCGGATAGCACTTATACTTGGCATGACGTCGGc                                                                                                                                                                                                                                                   >  2:93121/6‑251 (MQ=255)
      gTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCAtt                                                                                                                                                                                                                                            >  1:154899/1‑251 (MQ=255)
       tAACGCTGGCTGCTCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTAAATCCAGGTGATTGGCAGAGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTGTTATTGGCCTGACGTCGG‑AGCCAtt                                                                                                                                                                                                                                            >  1:398865/1‑250 (MQ=255)
                             gTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGtt                                                                                                                                                                                                                     <  1:22468/251‑1 (MQ=255)
                                                  gATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGc                                                                                                                                                                                                <  1:324577/251‑1 (MQ=255)
                                                         gACTCAATACAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTCGGCTTGCCAAGCGGCTCTATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTCTTATTGGCCTGACGTCGG‑GGCCCTTATGATGATGTTTCGTATGCGGTTCCTGCACCGTCTGCCGTCAGCCATCAtt                                                                                                                                                                                         >  2:202447/1‑251 (MQ=255)
                                                                 caagttGATTGGCAATGGGATTTTGCGTGGTTATAAAGTTACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGAGCTTGCCATGCGGCTCTATTCTAGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCAATTATGATGATGTTGCGTATGCGGTTCCTGCACCGTCTGCCGTCAGCCATCATTCTGCAACg                                                                                                                                                                                 >  2:68842/6‑251 (MQ=255)
                                                                  cAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGG‑CCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACg                                                                                                                                                                                 >  2:36683/1‑249 (MQ=255)
                                                                                                     aGATACGCGTTCCATTTTCTATATTGCCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCAc                                                                                                                                             <  1:302386/251‑1 (MQ=255)
                                                                                                                ccagtttCTATATTACCTTTACGGTTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCt                                                                                                                                  <  1:253195/247‑1 (MQ=255)
                                                                                                                                 tttACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGACAATGAACGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTATGCAACGAGCATCCCGCTAATAAAGACAAGCCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCt                                                                                                                 >  1:232460/1‑251 (MQ=255)
                                                                                                                                      ggCTTACTGTGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATGCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGc                                                                                                            <  1:380892/251‑1 (MQ=255)
                                                                                                                                                                      atatTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCATGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTa                                                                             <  1:354704/250‑1 (MQ=255)
                                                                                                                                                                                    aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTac                                                              >  2:4164/1‑251 (MQ=255)
                                                                                                                                                                                            ggTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTacac                                                            <  1:377927/245‑1 (MQ=255)
                                                                                                                                                                                                  tGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCg                                                 >  1:388104/1‑250 (MQ=255)
                                                                                                                                                                                                           gggnCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCt                                       <  2:375213/251‑1 (MQ=255)
                                                                                                                                                                                                                                    tttATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCgg              >  1:118/1‑251 (MQ=255)
                                                                                                                                                                                                                                                cTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCCCCtt  >  1:250822/1‑251 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 
GAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCTCAACGTGTATATCCCCGGTAACTTCCCCTT  >  NC_000913/1744501‑1744992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑