Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,898,302	G→A	G94D (GGT→GAT)	cbrC →	PF03691 family colicin E2 tolerance protein CbrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,898,302	0	G	A	100.0%	69.3 / NA	25	G94D (GGT→GAT)	cbrC	PF03691 family colicin E2 tolerance protein CbrC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/8);  total (17/8)

ACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGATATAAAAGATCGCCTCGATGAATTTGCCAACCTTGAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTG  >  NC_000913/3898072‑3898545                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aCTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGCAAAGTTTGCAGGTATTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTACGAACACATCTCCtg                                                                                                                                                                                                                                 >  1:77452/1‑251 (MQ=255)  cTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCtg                                                                                                                                                                                                                                 >  2:279906/1‑250 (MQ=255)                aTTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGaa                                                                                                                                                                                                                    >  1:10269/1‑249 (MQ=255)                         ggATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAgttggttg                                                                                                                                                                                                         <  1:339961/251‑1 (MQ=255)                                   gTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGcc                                                                                                                                                                                               >  1:412684/1‑251 (MQ=255)                                          gCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCt                                                                                                                                                                                                                       >  2:93279/1‑220 (MQ=255)                                          gCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCt                                                                                                                                                                                                                       <  1:93279/220‑1 (MQ=255)                                                           caGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGGGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAgg                                                                                                                                                                        >  2:91749/1‑250 (MQ=255)                                                              aCGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAAtt                                                                                                                                                                    >  2:168025/1‑251 (MQ=255)                                                              aCGTCAGTTTATTACTCGGGTCCATTTTATTGCGTTGATGAAGTTGAACATCTCTGCCCGTGGTGTATTGCGGACGGTTCTGTTGCTGAACAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGAGTTGAATTTGTGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAAtt                                                                                                                                                                    >  2:74632/1‑251 (MQ=255)                                                                   aGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGc                                                                                                                                                               >  1:367825/1‑251 (MQ=255)                                                                                    ccTTTTATTGCGTTGATGTAGTTGAACATCTCTGTCCGTTGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGTTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGAt                                                                                                                                              <  2:312610/251‑1 (MQ=255)                                                                                                   atgaAGTTGAACATCTCTGGCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGGTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGGTGAATTTGAGTATGATGAAGAGGACGAAGTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTAGCATGGATGGCAGCAGGAATTCTGGCGCGCGCATTGTGGCGATTTCTGTGTTGTTATc                                                                                                                               <  2:77452/251‑1 (MQ=255)                                                                                                              cATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTggg                                                                                                                    >  1:103688/1‑251 (MQ=255)                                                                                                                                       aCGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTGTCAGGATGAAGCCAGCAGAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTTGAATGATATAAAATATCGCCTc                                                                                           <  1:91398/251‑1 (MQ=255)                                                                                                                                                                        ttCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCgg                                                                                                                             >  2:41372/1‑184 (MQ=255)                                                                                                                                                                        ttCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCgg                                                                                                                             <  1:41372/184‑1 (MQ=255)                                                                                                                                                                                gatgCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGATATAAAAGATCGCCTCGATGAATTTGCCAACCTTGAAGAAGATTGTGAGAATTTCgg                                                  >  2:44704/1‑251 (MQ=255)                                                                                                                                                                                     cAGCATCGAAGGTGTTGAATTTGAGTATGATGAAGAGAACGAGTTTGCAGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTATGGGTTGTTATCGGCTATGTGGGCTGGAATGATCTAAACGATGGCCTAGATGAATTTGCCAACCTTGAAGAAGATTGTGAGAATTTCGGTAtt                                              >  2:400134/1‑250 (MQ=255)                                                                                                                                                                                     cAGCATAGAAGGCGTTGTATTTGAGAATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGGATGATATAAAAGATCGCCTCGATGAATTTGCAAACCTTGAAGCAGATTGTGAGAATTTCGGTAtt                                              >  2:400152/1‑250 (MQ=255)                                                                                                                                                                                           aGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGTGCTGGAATGATATAAAAGATCGCCGCGATGAATTTGCCAACCTTGAAGAAGATTGTGAGAAGTTCGGTATTAGAAAt                                        <  2:200868/250‑1 (MQ=255)                                                                                                                                                                                             aaGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGATATAAAAGATCGCCTCGATGAATTTGCCAACCTTGAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCt                                     <  1:147444/251‑1 (MQ=255)                                                                                                                                                                                                                      agagGACGAATTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGATATAAAAGATCGCCTCGATGAATTTGCCAACCTTGAAGAGGATTGTGAGAATTTCGGTAGTAGAAATTCTGATCTAGCTAAATGCCTGCaaaatg            >  2:145173/1‑249 (MQ=255)                                                                                                                                                                                                                             taaTTTGCCGATATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGATATAAAAGATCGCCTCGATGAATTTGCCAACATTGAAGAAGATTGTTAGAATTTCGGTCTTAGAAATTCTGATCTAGCTAAATGACTGCCAACGGGTGGTc      >  2:120680/2‑250 (MQ=255)                                                                                                                                                                                                                                tttGCCGATATTAAGAACACATATCATGATGAAATGCTGAAAGAGTTGGTTGAAAGAACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGATATAAAAGATCGCCTCGATGAATTTGCCAACCTTGAAGAATATTGTGAGAATTTCGGCATTAGTAATTCTGATCTAGCTAAATGCCTGCAAAAGGGAGGTCATTg  >  1:228971/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ACTGGAAACAGGCGCATTTGAACAGGATAAAACCGTAGAGTGCGATTGCTGTGAACAACAGACGTCAGTTTATTACTCGGGTCCCTTTTATTGCGTTGATGAAGTTGAACATCTCTGTCCGTGGTGTATTGCGGACGGTTCTGCTGCTGAAAAGTTTGCAGGTAGTTTTCAGGATGATGCCAGCATAGAAGGTGTTGAATTTGAGTATGATGAAGAGGACGAATTTGCCGGTATTAAGAACACATATCCTGATGAAATGCTGAAAGAGTTGGTTGAACGCACGCCAGGTTATCATGGATGGCAGCAGGAATTCTGGCTCGCGCATTGTGGCGATTTCTGTGTTTTTATCGGCTATGTGGGCTGGAATGATATAAAAGATCGCCTCGATGAATTTGCCAACCTTGAAGAAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTG  >  NC_000913/3898072‑3898545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑