Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	621,783	G→A	A136V (GCC→GTC)	fepD ←	ferric enterobactin ABC transporter membrane subunit FebD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	621,783	0	G	A	100.0%	170.2 / NA	49	A136V (GCC→GTC)	fepD	ferric enterobactin ABC transporter membrane subunit FebD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/24);  total (25/24)

GCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTAAACCGGCAAGGGTACGCGGCAGCCGCGCGTCG  >  NC_000913/621543‑622019                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             gcgcgACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAAAATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGAGGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAAcaatcaa                                                                                                                                                                                                                                    <  1:71435/251‑1 (MQ=255)  cgcgACTCAGTAATAGCTCAGTTGCTCCGGCGATCAGCACCGGGATCAGCCCCACTTTTAAGGTTTGTAGATTGCGAATATCCAGCGACCCGGCTTGCCAGAACCCCACCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCTCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAAcaatcaat                                                                                                                                                                                                                                   <  2:257835/251‑1 (MQ=255)      aCTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAgg                                                                                                                                                                                                                               >  1:91763/1‑251 (MQ=255)                    cagtggccccGGGGATCGGCACCGGGATCAGCACCACTTTTAAGGTATGTAGAGTGCGAATATCCAGCGACCCGGCTTGCCAGAAACGCAACTGCTAGTAGACGTCAGGATGAAGCGGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCg                                                                                                                                                                                                                 <  2:158659/243‑1 (MQ=255)                    cAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCg                                                                                                                                                                                                                 >  1:177527/1‑251 (MQ=255)                                    cAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGcc                                                                                                                                                                                                  <  1:336804/250‑1 (MQ=255)                                           gggATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTc                                                                                                                                                                                          >  1:217084/1‑251 (MQ=255)                                             gATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCt                                                                                                                                                                                        >  2:188367/1‑251 (MQ=255)                                                cAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAAGGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTgc                                                                                                                                                                                      <  1:19857/250‑1 (MQ=255)                                                      cacTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGa                                                                                                                                                                                 >  1:340838/1‑249 (MQ=255)                                                          tttAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCACCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGCCCATCGCCACTTGTTCCTGCGCGGAAGAGt                                                                                                                                                                            >  1:270574/1‑250 (MQ=255)                                                           ttAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTa                                                                                                                                                                           >  1:71292/1‑250 (MQ=255)                                                               ggTATGTAGATTGCGAATATCCAGCGACCCGTCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGAGGCCGCTGGCC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGACTAAGGCCATCGCCAGTTGTTCCGGCGCGGAAGAGTAACCaa                                                                                                                                                                      <  1:308870/251‑1 (MQ=255)                                                                  aTGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAACCGCAACTGATCGTAGACGTCAGGATTAACCGGGGCGATGCCGCTGGTC‑ATCCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACa                                                                                                                                                                   <  2:239165/251‑1 (MQ=255)                                                                    gTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAgc                                                                                                                                                                 <  2:177527/251‑1 (MQ=255)                                                                             gAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCAccc                                                                                                                                                        >  2:392649/1‑251 (MQ=255)                                                                               atatCCAGCGAACCGGCCTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCAccc                                                                                                                                                        >  1:35097/1‑249 (MQ=255)                                                                                     aGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAAt                                                                                                                                                >  2:277637/1‑251 (MQ=255)                                                                                        gAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGc                                                                                                                                             >  2:29809/1‑251 (MQ=255)                                                                                              gCTTGCAAAAAACGCAACGGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCAACGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGTCAAAGCt                                                                                                                                       <  1:117920/251‑1 (MQ=255)                                                                                                 tGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTg                                                                                                                                      <  1:206288/249‑1 (MQ=255)                                                                                                 tGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGACGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGc                                                                                                                                    >  1:302262/1‑251 (MQ=255)                                                                                                       aaaCGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGc                                                                                                                              <  1:212373/251‑1 (MQ=255)                                                                                                             aaCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCAc                                                                                                                        >  1:105904/1‑251 (MQ=255)                                                                                                                tGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGcc                                                                                                                     >  1:90903/1‑251 (MQ=255)                                                                                                                            gcAGGATTAAGCAGGGCACTGCGGC‑GGTCCAGTCCTTCCACCACCGCCGCCACCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAGCTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCTCAAGGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCggg                                                                                                         <  2:17892/250‑1 (MQ=255)                                                                                                                                        gggtcGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTgg                                                                                             <  1:76458/247‑1 (MQ=255)                                                                                                                                                  cGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTg                                                                                   <  1:188367/251‑1 (MQ=255)                                                                                                                                                      ggTC‑AGTCCTTCCAGCCCCGCCGCCAGCGCCACGCACGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAggg                                                                               <  2:349454/251‑1 (MQ=255)                                                                                                                                                        cc‑AGTCCTCCCGGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGCCTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTGCCTGCGCGGAAGAGTAACCAAACATCGCCGCGCCCAGCACAACGGCAAAGCCCGTCCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGtt                                                                             <  1:47942/250‑1 (MQ=255)                                                                                                                                                           aGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTg                                                                           >  1:121693/1‑251 (MQ=255)                                                                                                                                                                                 gcgcCACGCCCGCGAGGGTTAATCACACCGGACTTAACTGGCCGCCGCCCGGTCTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCTCGGCGAAGGCCTTCGCCGGGTGTTCCTGCGCGGAAGAGTGACCAAACATCGCCGCACCCAGTACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGGCGGCAGGTGGGTTTCGGGTGATGGTTTGCATGAACTCCCCGGCAAGGCCa                                                     <  2:185057/251‑1 (MQ=255)                                                                                                                                                                                  cgcCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCa                                                     >  1:400005/1‑250 (MQ=255)                                                                                                                                                                                    ccACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAgc                                                  <  1:376389/251‑1 (MQ=255)                                                                                                                                                                                     cACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAgcg                                                 >  2:107877/1‑251 (MQ=255)                                                                                                                                                                                       cgcccgccAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCg                                                                                                     <  2:157673/197‑1 (MQ=255)                                                                                                                                                                                       cgcccgccAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCg                                                                                                     >  1:157673/1‑197 (MQ=255)                                                                                                                                                                                            gccAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCAt                                                                        <  1:103531/221‑1 (MQ=255)                                                                                                                                                                                            gccAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGGTTGCAt                                                                        >  2:103531/1‑221 (MQ=255)                                                                                                                                                                                               aGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGc                                       >  1:163358/1‑251 (MQ=255)                                                                                                                                                                                                    tAAACGCCCCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAATCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGt                                   <  2:270826/250‑1 (MQ=255)                                                                                                                                                                                                    tAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGt                                   >  2:363549/1‑250 (MQ=255)                                                                                                                                                                                                      aaCGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTaa                                 >  2:101571/1‑250 (MQ=255)                                                                                                                                                                                                        cGCACCGGACTTAACTGCCCGCCGCCCTGCCTGCCGGTAAAGACACCAACCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGGTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAGGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAGCGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTAAAcc                              <  2:294218/251‑1 (MQ=255)                                                                                                                                                                                                             cGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTAAACCGGCa                          >  2:73489/1‑250 (MQ=255)                                                                                                                                                                                                                  ttAACTGCCCGCCGCCCTGACTGCCGGTAAAGACAACACTCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCACGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTAAACCGGCAAgggg                     >  2:43743/1‑249 (MQ=255)                                                                                                                                                                                                                     aCTGCCCGCCCCCCTGACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCAAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTAAACCGGCAAGGGTAcgc                 <  2:71292/251‑1 (MQ=255)                                                                                                                                                                                                                                    gACTGCCGGTAAAGACAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTAAACCGGCAAGGGTACGCGGCAGCCGCGCGTCg  >  1:281138/1‑251 (MQ=255)                                                                                                                                                                                                                                    cccggCCGGTAGAGACAACAATCAACGAGGCCACCAGCGTGCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCTCACCCAGCACAAGGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTGGGAAAGTGGTTTGCGGGTGAGGGTTTGCATGAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTAAACCGGCAAGGTTACGCGGCAGCCGCGCGTCg  <  2:186284/247‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             GCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTC‑AGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGCAACAATCAATGAGGCCACCAGCGCCCCGGCGAAGGCCATCGCCAGTTGTTCCTGCGCGGAAGAGTAACCAAACAGCGCCGCACCCAGCACAATGGCAAAGCTGGCTCCGGCGTTCACGCCAAGCAAGCCGGGGTCGGCAAGTGGGTTTCGGGTGAGGGTTTGCATTAACGCCCCGGCAAGGCCAAGCGCGCCGCCTGCCAGTAAACCGGCAAGGGTACGCGGCAGCCGCGCGTCG  >  NC_000913/621543‑622019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑