Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,592,893	G→A	intergenic (‑451/+5724)	hipB ← / ← lsrK	antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,592,893	0	G	A	100.0%	151.7 / NA	42	intergenic (‑451/+5724)	hipB/lsrK	antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/19);  total (23/19)

GCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGGAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATA  >  NC_000913/1592651‑1593120                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    gCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCAc                                                                                                                                                                                                                              <  1:248960/250‑1 (MQ=255)       tataCGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTGCGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGt                                                                                                                                                                                                                         <  1:123750/249‑1 (MQ=255)                  ccAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCat                                                                                                                                                                                                            <  2:22890/251‑2 (MQ=255)                                   ccAGCATACCCTGAGTATCGCTCTAGCCTTTATCACCTAGTTGCACACCGACATTACCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTAATTCACCGAGATTACTCGCACCTTCACGGCCTACGGTTTGACAATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTACGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTc                                                                                                                                                                                           >  2:56387/1‑251 (MQ=255)                                                     cGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCAcccc                                                                                                                                                                          <  2:349195/250‑1 (MQ=255)                                                         ataGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCCAGTc                                                                                                                                                                     >  1:145970/1‑251 (MQ=255)                                                                 tATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTg                                                                                                                                                              <  1:318735/250‑1 (MQ=255)                                                                      ccTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCTCGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACAtt                                                                                                                                                        <  2:160751/251‑1 (MQ=255)                                                                             gCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCNTCg                                                                                                                                                 >  2:382447/1‑251 (MQ=255)                                                                                 acCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCtt                                                                                                                                             >  2:8384/1‑251 (MQ=255)                                                                                   cGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTcc                                                                                                                                           <  1:383939/251‑1 (MQ=255)                                                                                    gACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCATTccc                                                                                                                                          >  2:34201/1‑250 (MQ=255)                                                                                           ccccACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCTGTACGTACTTCCCCGAGATTACGCGCTCCTTCACGTCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTGCAATGTAAGGCTGAACCTCACGCTGTTTCCCATCGTCACGCTGGTGATGGCTGTTCAGGTAGCTTTTCACCCCAATTCGCGTTTGCCCATTGCCTTCGCCGTCCGTTTCAAt                                                                                                                                   <  2:46752/251‑1 (MQ=255)                                                                                               acaGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATgcgc                                                                                                                               <  1:264055/251‑1 (MQ=255)                                                                                                      aaGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGt                                                                                                                        >  1:245666/1‑251 (MQ=255)                                                                                                         gttgttATTTACTTTCTCCTAAACCCCGGTACGTAATTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACTATTCATCTTCACGGCGTAGGCTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGATGAAACTCACGCTGTTGACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGGTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCATTGCGCGTTCCGTCtt                                                                                                                     >  2:82115/1‑251 (MQ=255)                                                                                                         gttgttATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCtt                                                                                                                     <  2:245666/251‑1 (MQ=255)                                                                                                           tgttATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGttagattcggaagagcacacgtc                                                                                                                   >  1:321980/1‑230 (MQ=255)                                                                                                           tgttATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGtt                                                                                                                                        <  2:321980/230‑1 (MQ=255)                                                                                                                 ttACTTTCGCCTCAACCCCGGTACGGACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGAGTTCCGTCTTTCCGGGt                                                                                                              >  2:283983/1‑250 (MQ=255)                                                                                                                     tttCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGt                                                                                                         >  2:95648/1‑251 (MQ=255)                                                                                                                     tttCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGt                                                                                                         >  1:260598/1‑251 (MQ=255)                                                                                                                         gCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGTTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGGATGGTCgg                                                                                                      >  2:304063/1‑250 (MQ=255)                                                                                                                         gCCTCAACCCCGGGACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTCGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGAACATTTCCGACGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGttgt                                                                                                      >  2:360303/1‑247 (MQ=255)                                                                                                                             cAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCt                                                                                                 <  1:43135/251‑1 (MQ=255)                                                                                                                                  ccGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTcac                                                                                            <  2:145970/251‑1 (MQ=255)                                                                                                                                    ggTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGCAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCTTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTAGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCacac                                                                                          >  2:46094/1‑251 (MQ=255)                                                                                                                                                         aCGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCt                                                                     >  1:81788/1‑251 (MQ=255)                                                                                                                                                            cgcACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGGTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCtgtg                                                                  <  2:237071/251‑1 (MQ=255)                                                                                                                                                                  ttCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTgg                                                             >  1:251090/1‑250 (MQ=255)                                                                                                                                                                       ggCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTCTGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTAc                                                       >  1:203865/1‑251 (MQ=255)                                                                                                                                                                           tACGGTTTGACCATTCATCTTCACCGCGTAGACTTTACTATTGTTGAACCACTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGCGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCCTCCGTTTCAATGCGCGTTCCGTCTTTCCGTGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTAcccgg                                                   >  2:200043/1‑248 (MQ=255)                                                                                                                                                                                   gACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCAGCCAGGTGATTTGCGCCTGTGGCTTGACGTACCAGGTATTCAGc                                           <  1:150081/251‑1 (MQ=255)                                                                                                                                                                                       aTTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCAAGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCTGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGCATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTcc                                       >  1:84363/1‑251 (MQ=255)                                                                                                                                                                                          cATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCtt                                    <  1:382447/251‑1 (MQ=255)                                                                                                                                                                                               tCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTg                               >  2:316631/1‑251 (MQ=255)                                                                                                                                                                                                        aGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGCATTCAGCGTCCCTTCGCTGCCGCTAAAt                      >  1:303687/1‑251 (MQ=255)                                                                                                                                                                                                               gCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTccc                <  1:134255/250‑1 (MQ=255)                                                                                                                                                                                                                  aTTGTTGATCCAGGTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTGTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGTCTGGACGGACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGc              <  2:169800/249‑1 (MQ=255)                                                                                                                                                                                                                        gATCCAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAgg      >  2:154379/1‑251 (MQ=255)                                                                                                                                                                                                                            cAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGtat   >  2:321506/1‑250 (MQ=255)                                                                                                                                                                                                                            cAGTTCGCTTCAATGTAAGGCTGAAACTCACGCTGTTTACCACCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCATGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGtata  <  1:308781/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    GCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTTATTTACTTTCGCCTCAACCCCGGTACGTACTTCACCGAGATTACGCGCACCTTCACGGCCTACGGTTTGACCATTCATCTTCACGGCGTAGACTTTGCTATTGTTGATCCAGTTCGCTTCAATGTAAGGCTGGAACTCACGCTGTTTACCATCGTCACGCTGGTGATGGCTGTTCAGGTAGGTTTTCACCCCAAGTCGCGTTTGCACATTTCCGTCGCCTTCCGTTTCAATGCGCGTTCCGTCTTTCCGGGTATGGTCGGAATCTTTCACACCCATCCAGGTGATTTGCGCCTGTGGCTGGACGTACCAGGTATTCAGCGTCCCTTCGCTGCCGCTAAATGTTCCCGCTTCAAAGGTATA  >  NC_000913/1592651‑1593120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑