Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,564,733	G→A	Q617* (CAG→TAG)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,564,733	0	G	A	100.0%	171.7 / NA	50	Q617* (CAG→TAG)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

GTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGTTTGATTTGTACATCGAACAACGCCTTTG  >  NC_000913/3564495‑3564960                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                    gtgtTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCgttg                                                                                                                                                                                                                           <  2:139609/249‑1 (MQ=255)    ttACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGAt                                                                                                                                                                                                                      >  2:153258/1‑251 (MQ=255)     tACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATc                                                                                                                                                                                                                     >  1:134272/1‑251 (MQ=255)           caaTAAAGACATTGTCAGCACCGACATGATCCAGCATCTCGACATTAGCACCGTCAAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTAATGGTGACGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGGATGATCAACTGCGACCGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTc                                                                                                                                                                                                               >  2:235966/2‑251 (MQ=255)              tAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCt                                                                                                                                                                                                            >  1:83741/1‑251 (MQ=255)               aaaGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCt                                                                                                                                                                                                            <  1:47003/250‑1 (MQ=255)                    tatTGTCCGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTc                                                                                                                                                                                                       >  1:136322/1‑250 (MQ=255)                        gTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGa                                                                                                                                                                                                  <  2:94856/251‑1 (MQ=255)                          cAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTGATGTTACTGGTGCCGGAAGCTGCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGAt                                                                                                                                                                                                 <  1:127/250‑1 (MQ=255)                                         ccAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAAGAAtgtg                                                                                                                                                                                  <  1:98774/250‑1 (MQ=255)                                          cAGNATCTCGACGGTCGCACCGTCCAACGTACGCATAGTCAGCGCGCCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACATATCTGCCGCCGGAATGATCAACTGCGCCAGGCGAAGGCTGTAGTTCGGGATGAACACGACTTTCATCTTATCGCCAATCTACGGATCGTTGTTGTTCACTTTCGCTTCGTCGTTGATCAAATGAATAAtgttct                                                                                                                                                                                <  2:368117/251‑4 (MQ=255)                                          cAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCt                                                                                                                                                                                <  1:167142/251‑1 (MQ=255)                                             cATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCg                                                                                                                                                                             <  1:352963/251‑1 (MQ=255)                                                   gACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGt                                                                                                                                                                       >  1:360434/1‑251 (MQ=255)                                                         cGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAgg                                                                                                                                                                 <  1:375605/251‑1 (MQ=255)                                                            cccGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCg                                                                                                                                                              <  1:101691/250‑1 (MQ=255)                                                             ccGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCg                                                                                                                                                              >  2:48412/1‑250 (MQ=255)                                                              cGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGaa                                                                                                                                                            <  2:273170/251‑1 (MQ=255)                                                              cGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGa                                                                                                                                                             >  2:335054/1‑250 (MQ=255)                                                                tCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACgg                                                                                                                                                                                                                                  <  2:153452/179‑1 (MQ=255)                                                                tCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACgg                                                                                                                                                                                                                                  >  1:153452/1‑179 (MQ=255)                                                                      gTACCGATAGTCAGCGCACCGTTAAGCGCACATTTCATGTTACTTGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAAGCTGTTCAGACAGATCTGCCGCCGTAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCGTGTAAAAGTCCGAAGCCGCCtt                                                                                                                                                    <  2:386337/251‑1 (MQ=255)                                                                         ccGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTAcc                                                                                                                                                 >  2:342496/1‑251 (MQ=255)                                                                              aGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGcc                                                                                                                                                                           >  2:83815/1‑220 (MQ=255)                                                                              aGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGcc                                                                                                                                                                           <  1:83815/220‑1 (MQ=255)                                                                                        ccGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCAc                                                                                                                                  >  2:161506/1‑251 (MQ=255)                                                                                        ccGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCGTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCAc                                                                                                                                  <  1:38317/251‑1 (MQ=255)                                                                                                                    ggCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCg                                                                                                      <  1:199181/250‑1 (MQ=255)                                                                                                                      ccGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGc                                                                                                    >  1:158906/1‑251 (MQ=255)                                                                                                                              ttCCGTCCCTGCCAGCGCAATATGTTCAGACAGATCTGCCGACGGCATGATCAACTGCGCAAGGATAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCATTACCGCCAAAAATATTCACGCGAGGTACCCACTTCGCATCCGGGTCGGCCTTGATGc                                                                                            >  2:398779/1‑251 (MQ=255)                                                                                                                                   tCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGtt                                                                                        >  2:109298/1‑250 (MQ=255)                                                                                                                                                aaTCTGTTCAGACAGATCTGCCGCCGGAATGCTCAACTGCGCCCGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATc                                                                           >  1:141170/1‑250 (MQ=255)                                                                                                                                                aaTCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGGAATc                                                                           >  2:92783/1‑250 (MQ=255)                                                                                                                                                  tCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATcac                                                                         >  2:20058/1‑250 (MQ=255)                                                                                                                                                     gTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATcaca                                                                        <  1:44455/248‑1 (MQ=255)                                                                                                                                                       tCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCa                                                                    <  1:79735/250‑1 (MQ=255)                                                                                                                                                             agaTCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACAtt                                                             >  1:144459/1‑251 (MQ=255)                                                                                                                                                                       ccgGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTg                                                    >  2:28423/1‑250 (MQ=255)                                                                                                                                                                                  aaCTGCGCCAGGCTAACGATGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTc                                        >  1:324230/1‑251 (MQ=255)                                                                                                                                                                                        gcCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAAt                                  <  1:171155/251‑1 (MQ=255)                                                                                                                                                                                         ccAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGCCGTTTGTATTCGTGAATa                                 <  1:48412/251‑1 (MQ=255)                                                                                                                                                                                            ggCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGt                              <  1:121590/251‑1 (MQ=255)                                                                                                                                                                                              cTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAAGACGttt                            >  1:326122/1‑251 (MQ=255)                                                                                                                                                                                                aaCGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGttt                            <  1:289921/249‑1 (MQ=255)                                                                                                                                                                                                          ttCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGTTTGATTTGTACATc                >  1:345445/1‑251 (MQ=255)                                                                                                                                                                                                           tCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGTTTGATTTGTACATc                <  1:50396/250‑1 (MQ=255)                                                                                                                                                                                                           tCGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTCCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGTTTGATTTGTACATc                <  1:368961/250‑1 (MQ=255)                                                                                                                                                                                                            cGGGATGAACACGACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGACTACGTTTGATTTGTACATCg               >  2:309180/1‑250 (MQ=255)                                                                                                                                                                                                                       cGACTTTCAGCTTATCGCCAATCTACGGAGCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGTTTGATTTGTACATCTAACAACGCCttt   >  2:134891/1‑251 (MQ=255)                                                                                                                                                                                                                        gACTTTCAGCTTATCGCCAATCTACGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGTTTGATTTGTACATCGAACAACGCCTTTg  <  1:342496/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                    GTGTTACCAAAAATAAAGATATTGTCAGCACCGACATGATCCAGCATCTCGACATTCGCACCGTCCAACGTACCGATAGTCAGCGCACCGTTAAGCGCAAACTTCATGTTACTGGTGCCGGAAGCTTCCGTCCCTGCCAGCGAAATCTGTTCAGACAGATCTGCCGCCGGAATGATCAACTGCGCCAGGCTAACGCTGTAGTTCGGGATGAACACGACTTTCAGCTTATCGCCAATCTGCGGATCGTTGTTGATCACTTTCGCTACGTCATTGATCAAATGAATAATGTGCTTCGCCATGTAATAGGCCGAAGCCGCCTTACCGCCAAAAATATTCACGCGCGGTACCCACTTCGCATCCGGGTCGGCCTTGATGCGGTTATAGCGGGTAATCACATGCAACACATTCATCAATTGACGTTTGTATTCGTGAATACGTTTGATTTGTACATCGAACAACGCCTTTG  >  NC_000913/3564495‑3564960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑