Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,319,705	A→G	T357A (ACT→GCT)	argG →	argininosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,319,705	0	A	G	100.0%	249.4 / NA	67	T357A (ACT→GCT)	argG	argininosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/32);  total (35/32)

GAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGGCGTG  >  NC_000913/3319461‑3319944                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGTGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGt                                                                                                                                                                                                                                            <  1:146062/250‑1 (MQ=255)    cGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCATCCAGATCGCTGGTGa                                                                                                                                                                                                                                          >  1:253707/1‑249 (MQ=255)     gACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGt                                                                                                                                                                                                                                       <  1:159763/251‑1 (MQ=255)       ccAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGGGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTa                                                                                                                                                                                                                                     <  2:189382/251‑1 (MQ=255)                 aaCCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCt                                                                                                                                                                                                                           >  2:10706/1‑251 (MQ=255)                    cGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTgcg                                                                                                                                                                                                                        <  1:312498/251‑1 (MQ=255)                       atnatcGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCTTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCGGGTGAAGTTACCCTGGAGCTGCGcc                                                                                                                                                                                                                      <  2:382890/250‑1 (MQ=255)                             gAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGa                                                                                                                                                                                                                >  2:7558/1‑250 (MQ=255)                              aaGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATTAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGTATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAAc                                                                                                                                                                                                              <  2:284815/251‑1 (MQ=255)                                 cGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGat                                                                                                                                                                                                           <  1:121719/251‑1 (MQ=255)                                  gAAAAGCCGTGGAATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGACAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGatt                                                                                                                                                                                                          >  2:235780/1‑251 (MQ=255)                                      agacGTGGTATTTACGAAGCTGCGGGAATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTAACAACAAAAACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGCCTCTCTGCAACGCTGGGTTGCCAGCCAGATAGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTc                                                                                                                                                                                                      >  2:172603/4‑251 (MQ=255)                                      aGCCGTGGTATTTACGACGCTCCGGCGCTGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTCTCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGTAACGCTGGGTTGCCAGCAAGATCGCTGGTGCAGTTACCCTGGCGCTGCGCCGTGGGAACGATTATTc                                                                                                                                                                                                      >  2:160748/1‑251 (MQ=255)                                                tttACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATGACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCGCATGCGCTGAGGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGaa                                                                                                                                                                                             <  2:140700/250‑1 (MQ=255)                                                               ggATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAAc                                                                                                                                                                             >  2:54175/1‑251 (MQ=255)                                                               ggATGGCACTGCTGCACATTGCGTATGAACGCCTGTGGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAAc                                                                                                                                                                             <  2:130044/251‑1 (MQ=255)                                                                 aTGGCACTGCTGCACATTGCGTATGTACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATTGTCGTCAGTTGGGCCGTCTTCTGTACCAGGGGCGTTGGTTTGACTCCCAGTCGCTGATGCTGCGTGACTCTCTGCAACGCTGGTTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGTGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCt                                                                                                                                                                           <  1:235780/251‑1 (MQ=255)                                                                  tGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCt                                                                                                                                                                           <  2:261667/250‑1 (MQ=255)                                                                  tGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCt                                                                                                                                                                           >  1:261667/1‑250 (MQ=255)                                                                  tGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTg                                                                                                                                                                          >  1:341746/1‑251 (MQ=255)                                                                   ggCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTg                                                                                                                                                                          >  1:317512/1‑250 (MQ=255)                                                                       ctgctgCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCt                                                                                                                                                                      >  1:373871/1‑250 (MQ=255)                                                                        tgctgcACATTGAGTCTGAACGCCTGTTGACCGGTATTCACAACGAAGACACCCTTCAGCAGTATCACTCGCATGGTCGTCAGTTGGCCAGCCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCCACGCTGGGCTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACNTAc                                                                                                                                                                    >  2:372122/1‑251 (MQ=255)                                                                           tgcACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACa                                                                                                                                                                   >  1:261852/1‑249 (MQ=255)                                                                              acaTTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGcc                                                                                                                                                               >  1:108019/1‑250 (MQ=255)                                                                               aaTTGAGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCTAGATCGCTGGTGAAGTTACCATGGATCTGCGCCGTGGGAACGATTATTCAATCCTGAATGCCGTCTCAGAGAACCTGACCTTCAAGca                                                                                                                                                               <  2:253707/248‑2 (MQ=255)                                                                                 ttGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGACGTGGGAACGATTATTc                                                                                                                                                                                                      >  1:56662/1‑208 (MQ=255)                                                                                 ttGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGGCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGTAGCTGCGCCGTGGGAACGATTATTc                                                                                                                                                                                                      <  2:56662/208‑1 (MQ=255)                                                                                 ttGCGTATGAACCCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGGATGGTCGTCAGTTGGGCCGTCTGCTGGTCCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAAt                                                                                                                                                                                            <  2:25547/218‑1 (MQ=255)                                                                                         gcaCGCCTGTTGACCTGTATTCACACCGAAGACACAATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGATGTAACAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTATGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGCACGATTATTCACTCCTGAATACCGTATCAGAGACCCTGACATAAAAGCCAGAGCGTCTGa                                                                                                                                                    >  2:416465/3‑250 (MQ=255)                                                                                         gAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGTAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGAc                                                                                                                                                   <  1:160748/251‑1 (MQ=255)                                                                                         gAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGAc                                                                                                                                                   <  1:267826/251‑1 (MQ=255)                                                                                              ccTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATgg                                                                                                                                              <  1:402788/251‑1 (MQ=255)                                                                                                 gTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGACCGATTATTCAAGCCTGAATACCGTCTCAGAGAACCTGACCTACAAGGCAGAGCGTCTGACGATGGaa                                                                                                                                            <  1:228387/250‑1 (MQ=255)                                                                                                    gACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGaaaa                                                                                                                                          >  1:211962/1‑249 (MQ=255)                                                                                                      ccGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGTCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACACTGTAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGCCCTACACGCCAGAGCGTCTGCCGATGGAAAAAgac                                                                                                                                      >  2:46901/1‑249 (MQ=255)                                                                                                       cGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCg                                                                                                                                     <  1:10706/251‑1 (MQ=255)                                                                                                          tATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACt                                                                                                                                  >  1:69585/1‑251 (MQ=255)                                                                                                          tATTCACAACGAAGACACCATTGAGCAGTATCACGAGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCAGACGATGGAAAAAGGAGACt                                                                                                                                  >  1:69573/1‑251 (MQ=255)                                                                                                              cacaACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGgt                                                                                                                              >  2:40915/1‑251 (MQ=255)                                                                                                                  aCGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTtc                                                                                                                          <  1:340440/251‑1 (MQ=255)                                                                                                                     aaGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCg                                                                                                                       >  1:298610/1‑251 (MQ=255)                                                                                                                              ttGAGCAGTATCACACGCATGGTCGTAAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGTCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCCgatgac                                                                                                              >  2:250831/1‑250 (MQ=255)                                                                                                                                gAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCg                                                                                                            <  2:142862/251‑1 (MQ=255)                                                                                                                                             cgcATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTg                                                                                               >  2:211282/1‑251 (MQ=255)                                                                                                                                             cgcATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTg                                                                                               <  1:7558/251‑1 (MQ=255)                                                                                                                                               cATGGTCGTCAGTTGTGCCGGCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACTATGGGAAAAGGCGACTCGGTGTGCTCGCCAGATGATCGTATTGGTCAATTGAc                                                                                             <  2:305865/251‑1 (MQ=255)                                                                                                                                               cATGGACGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCGCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGACCCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGAc                                                                                             <  2:31878/251‑1 (MQ=255)                                                                                                                                                         aGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGc                                                                                                                                                          >  2:13208/1‑180 (MQ=255)                                                                                                                                                               gCCGTCTGCTGTAAAAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCAAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTCCAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGCTGATCGTATTGGTCAATTGACCATGCGTAACCTGGat                                                                             >  2:391809/1‑251 (MQ=255)                                                                                                                                                                     tgctgTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACt                                                                       >  1:245673/1‑251 (MQ=255)                                                                                                                                                                     tgctgTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACt                                                                       >  1:217015/1‑251 (MQ=255)                                                                                                                                                                               ggggCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCcgcg                                                             <  1:39864/251‑1 (MQ=255)                                                                                                                                                                                    gTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGaaa                                                        <  2:340364/251‑1 (MQ=255)                                                                                                                                                                                         tttGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAgatg                                                                                                                >  2:114112/1‑190 (MQ=255)                                                                                                                                                                                         tttGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAgatg                                                                                                                <  1:114112/190‑1 (MQ=255)                                                                                                                                                                                                 ccAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATg                                           <  1:110270/251‑1 (MQ=255)                                                                                                                                                                                                  cAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGTGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTTACCTACAAGCCAGATCGTCTGACGATGGAAAAAGTCGAGTCGTTGTTCTCGCCAgat                                                                                                                 <  2:368262/180‑1 (MQ=255)                                                                                                                                                                                                  cAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATAGCTGGTGAAGTTACCCTGGAGCTGCGACGTGGGAACGATTATTCAATCCTGAATACCGTCTCCGCGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAgat                                                                                                                 >  1:368262/1‑180 (MQ=255)                                                                                                                                                                                                    ggCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGcc                                         >  1:332542/1‑250 (MQ=255)                                                                                                                                                                                                         tGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTgg                                                                               <  1:194224/207‑1 (MQ=255)                                                                                                                                                                                                         tGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTgg                                                                               >  2:194224/1‑207 (MQ=255)                                                                                                                                                                                                            tgctgcGTGACTCTCTGCAACGCTGGCATGCCAGCCAGATCGCTGGCGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGCGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGCGAAACTTTTCGGTTATGCCAAAACTGGc                                >  2:255817/1‑251 (MQ=255)                                                                                                                                                                                                                 cGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCt                           >  2:407572/1‑251 (MQ=255)                                                                                                                                                                                                                             cAACGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCGGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCTTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTTCTTTCCTCCTCTGc               <  2:171016/251‑1 (MQ=255)                                                                                                                                                                                                                              aaCGCTGGGTTGCCAGCCAGATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGcc              >  1:153422/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccagccagATCGCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGGCGTg  <  1:291031/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GAGCGACCAGATTGAAAACCGTATCATCGAAGCGAAAAGCCGTGGTATTTACGAAGCTCCGGGGATGGCACTGCTGCACATTGCGTATGAACGCCTGTTGACCGGTATTCACAACGAAGACACCATTGAGCAGTATCACGCGCATGGTCGTCAGTTGGGCCGTCTGCTGTACCAGGGGCGTTGGTTTGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGGCGTG  >  NC_000913/3319461‑3319944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑