Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,397,982	C→T	D133N (GAC→AAC)	yhdE ←	nucleoside triphosphate pyrophosphatase YhdE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,397,982	0	C	T	100.0%	163.8 / NA	46	D133N (GAC→AAC)	yhdE	nucleoside triphosphate pyrophosphatase YhdE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/16);  total (30/16)

CGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTT  >  NC_000913/3397752‑3398225                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cgcgTTTCCGAAGGCGTTACGTTTACTAACAATTCTGCCGTCATGTTTATCCCTTTTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATATCTGCCATTTATCGTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGa                                                                                                                                                                                                                                 <  1:199146/251‑1 (MQ=255)       tCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATATCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTc                                                                                                                                                                                                                            <  2:42273/250‑1 (MQ=255)            aGGCGTTACTTTTACTAACAATTCAGCCGTCATGTTTATCCCTTGTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACTTTCTGAAAGTCACATCg                                                                                                                                                                                                                      <  2:180583/251‑1 (MQ=255)               cGTNACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTg                                                                                                                                                                                                                   <  2:368068/251‑1 (MQ=255)                 ttACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGac                                                                                                                                                                                                                 >  1:259805/1‑251 (MQ=255)                   aCGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTAGTCTGTTAACGTTCTGAAAGTCACATCGGTGacc                                                                                                                                                                                                                >  1:2428/1‑250 (MQ=255)                                          cATGTTTATCCCTTTTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATATTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctgg                                                                                                                                                                                        >  2:189799/1‑251 (MQ=255)                                                            cACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCAct                                                                                                                                                                      >  1:61248/1‑251 (MQ=255)                                                               gCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCActgct                                                                                                                                                                   <  2:231530/251‑1 (MQ=255)                                                                      gTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtcatcc                                                                                                                                                            >  2:28200/1‑250 (MQ=255)                                                                              tACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTc                                                                                                                                                                                                                            >  1:138806/1‑179 (MQ=255)                                                                              tACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTc                                                                                                                                                                                                                            <  2:138806/179‑1 (MQ=255)                                                                                        ttCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCg                                                                                                                                          >  2:10285/1‑251 (MQ=255)                                                                                          cATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTCAAAGTCACATCGGTTACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCGCCTGATGGGTCTGACCCGAt                                                                                                                                        <  1:292046/251‑1 (MQ=255)                                                                                                    aaCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgcgc                                                                                                                               >  2:359734/1‑250 (MQ=255)                                                                                                          cGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCTCCCAGCCCCTTAATACCGGATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCAGCTCCTGATGGGTCTGACCCGATAATTTTCGCAACATCt                                                                                                                        <  2:175020/251‑1 (MQ=255)                                                                                                                        cGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGctct                                                                                                          <  2:93979/251‑1 (MQ=255)                                                                                                                         ggCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTg                                                                                                         >  1:186841/1‑251 (MQ=255)                                                                                                                            gTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGTCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCCCATGCTCTGCgc                                                                                                      >  2:320671/1‑250 (MQ=255)                                                                                                                            gTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGt                                                                                                      <  2:259805/251‑1 (MQ=255)                                                                                                                              gATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTgcg                                                                                                                     >  2:185540/1‑234 (MQ=255)                                                                                                                              gATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTgcg                                                                                                                     <  1:185540/234‑1 (MQ=255)                                                                                                                               aTAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTcgc                                                                                                   >  2:387707/1‑251 (MQ=255)                                                                                                                                     gCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGtt                                                                                             >  1:150451/1‑251 (MQ=255)                                                                                                                                        aTTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTATGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTtct                                                                                          >  1:221754/1‑251 (MQ=255)                                                                                                                                                         aaCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTc                                                                         >  1:6655/1‑251 (MQ=255)                                                                                                                                                            cgCCACCCAGCTCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCTCGATGTTTTCGTCTGTTAACGTTCGGAAAGTCACATCGGTGACCGCCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCAGCACCTGAGGGGTCTGACCCGATAATTTGCGCAACATCTACGCCGCATGCTCTGCGTCGTGCGGTGTCTCCAGcgttcgtcttgtcatg                                                                      <  2:51083/250‑16 (MQ=255)                                                                                                                                                                       cccTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGt                                                           >  1:373852/1‑251 (MQ=255)                                                                                                                                                                       cccTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGt                                                           >  2:339944/1‑251 (MQ=255)                                                                                                                                                                       cccTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGt                                                           >  1:191966/1‑251 (MQ=255)                                                                                                                                                                         cTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAg                                                                       >  2:315415/1‑237 (MQ=255)                                                                                                                                                                         cTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAg                                                                       <  1:315415/237‑1 (MQ=255)                                                                                                                                                                           gAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATcc                                                       >  2:345301/1‑251 (MQ=255)                                                                                                                                                                           gAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATcc                                                       >  2:59288/1‑251 (MQ=255)                                                                                                                                                                             aTACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGc                                                     >  2:220680/1‑251 (MQ=255)                                                                                                                                                                                       cACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCAcc                                           <  1:28200/251‑1 (MQ=255)                                                                                                                                                                                          cTGCTTTATCTAACGGTTCATCGCAGGCGACATAGCCCGAGATGTTTTCGTATGTTAACGTTCTGAACGTCACATCGGTGACCACCAGGCAATCGACATTGTACTGGCTGTCGGCCAAAGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCACCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCTCACCCAGCACCggg                                        >  2:238172/1‑251 (MQ=255)                                                                                                                                                                                                      aCGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCCCCGGGAGATCCTTCGcc                            <  1:10285/251‑1 (MQ=255)                                                                                                                                                                                                       cGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCg                           <  2:209586/251‑1 (MQ=255)                                                                                                                                                                                                       cGGTTCATCGCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATGTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCg                           >  2:205585/1‑251 (MQ=255)                                                                                                                                                                                                           tCATCGCTGGCGAAATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACAAGGCAATCGAGAATGTGGTGGCGGAAGGCCAACGCCACTGCTGTCATCACCTGATGGGTCAGACCCGCTAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGATTATCCAGCACTTCTCCGTTCAGGCTAACGATAGTATCCGCACCAAGCCACGGGAGATCCTTCGCAGTTTg                       >  2:264664/1‑251 (MQ=255)                                                                                                                                                                                                           tCATCCCTGGCGAAGTAGCCCGCGATGTTTGCGTCTGTTAACGTTCTGAAAGTCACGTCGGTGACCACCAGGCACTCGAGAATGTGCTGGCTGTCGGCCAACGCCACCGCGGTCATGACCCGATGGGTCTGACCCGATAATTTGCGCAACATCTACGCCGCATGCTCTGCGTAGCGCGGTTTCTCCAGCACTTCTCCGTTCATGAGAACGGTAGTATCCGATCCCAGCCCCGGGAGATCATTCGCCGTTTg                       >  2:364546/1‑251 (MQ=255)                                                                                                                                                                                                                gCTGGCGACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGcgcg                   >  1:247553/1‑250 (MQ=255)                                                                                                                                                                                                                     cGACATAGACCGAGATGTTTTCGTCTGTTAACGTTCTGAAAGTCAAATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGACAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCt             >  2:83157/1‑251 (MQ=255)                                                                                                                                                                                                                      gACATAGCCCGCGATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTg            >  2:275865/1‑251 (MQ=255)                                                                                                                                                                                                                                gagATGTTTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGGCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCACACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCttt  >  2:400980/3‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCACGCAGTGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCATGCTCTGCGTCGCGCGGTTTCTCCAGCACTTCTCCGTTCAGGATAACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTT  >  NC_000913/3397752‑3398225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑