Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	667,992	C→T	G76S (GGT→AGT)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	667,992	0	C	T	100.0%	124.7 / NA	35	G76S (GGT→AGT)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/18);  total (17/18)

TCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACCGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACT  >  NC_000913/667748‑668234                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   tCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACg                                                                                                                                                                                                                                              >  1:190407/1‑251 (MQ=255)                        aTTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTATATAATGCCGCGGCTgg                                                                                                                                                                                                                       <  2:245527/251‑1 (MQ=255)                                    gCGATCTACTTGTACTTCGGTCAGGTTAGTTGTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAggta                                                                                                                                                                                                           <  1:77062/251‑3 (MQ=255)                                     cGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCAc                                                                                                                                                                                                          >  1:33380/1‑251 (MQ=255)                                                 aCTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTGCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGAGAATGCCGCGGTTGATGGCGATAGGCACCAGCTTAATGc                                                                                                                                                                                               <  2:33658/250‑1 (MQ=255)                                                      ggTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGAGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGAGATAGGCACCAGCTTAATGCGGtttt                                                                                                                                                                                         >  2:280241/1‑251 (MQ=255)                                                                                agGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTa                                                                                                                                                               <  2:162569/251‑1 (MQ=255)                                                                                       aCGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCTGACAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACGTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCGCCAGCTTAATGCGGTTTTCATTAGAGCGGGTATGGTAGTCGGTAAAGCGaa                                                                                                                                                        <  2:301940/251‑1 (MQ=255)                                                                                                             tttCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGcgcg                                                                                                                                                                                                                  >  2:317383/1‑171 (MQ=255)                                                                                                             tttCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGcgcg                                                                                                                                                                                                                  <  1:317383/171‑1 (MQ=255)                                                                                                               tCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTgg                                                                                                                                >  2:143804/1‑251 (MQ=255)                                                                                                                   aaTGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCGGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGAt                                                                                                                            <  2:31889/251‑1 (MQ=255)                                                                                                                             gatCGTCAGCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCg                                                                                                                  <  2:317096/250‑1 (MQ=255)                                                                                                                                                                 cGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAg                                                                                          <  1:406311/239‑1 (MQ=255)                                                                                                                                                                 cGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAg                                                                                          >  2:406311/1‑239 (MQ=255)                                                                                                                                                                  gTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGccc                                                                             >  1:367531/1‑251 (MQ=255)                                                                                                                                                                    ccAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCgg                                                                           >  1:348600/1‑251 (MQ=255)                                                                                                                                                                             gctgcACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAAc                                                                  >  1:121330/1‑251 (MQ=255)                                                                                                                                                                                gcACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACa                                                                 <  2:190407/249‑1 (MQ=255)                                                                                                                                                                                         cGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGActct                                                      <  1:240561/251‑1 (MQ=255)                                                                                                                                                                                               tctcCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGt                                                 <  2:121330/250‑1 (MQ=255)                                                                                                                                                                                                     gCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTcg                                          <  2:83993/251‑1 (MQ=255)                                                                                                                                                                                                            ttCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGaaa                                     >  2:322984/1‑249 (MQ=255)                                                                                                                                                                                                                  cTGGTAGATAGTGCGGTTGAGGGCAAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGGAAAGCGAACAATTTGAAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCt                             >  2:107010/1‑251 (MQ=255)                                                                                                                                                                                                                       aGATAGTGCGGTTGAGGGCCAGAGGGATGCTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGt                        <  2:86987/251‑1 (MQ=255)                                                                                                                                                                                                                           aGTGCGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTc                     <  2:124122/250‑1 (MQ=255)                                                                                                                                                                                                                             tGCGGTTGAGGGCCAGAGGGATACTGTTAAGATCGCAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAACGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAACGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAACGAGTTCTGTCGTTTCATc                  >  2:58418/1‑251 (MQ=255)                                                                                                                                                                                                                               cGGTTGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCg                >  1:10306/1‑251 (MQ=255)                                                                                                                                                                                                                                   tGAGGGCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTg             <  1:371911/250‑1 (MQ=255)                                                                                                                                                                                                                                      gggCCAGAGGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTGCGtt         >  2:393624/1‑251 (MQ=255)                                                                                                                                                                                                                                        gCCAGAGGGATACTGTTACGATCGTAGATCATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCCGCGCCCGGCGCCCACACAGCGCGGACTCTGCCGTATAGTCGCGAAACGAGTTCTGTAGTTTCATCCGCTGCGtttt       >  2:189557/1‑251 (MQ=255)                                                                                                                                                                                                                                             agGGATACTTTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACt  <  2:217762/251‑1 (MQ=255)                                                                                                                                                                                                                                             agGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAg                                                                                                                                     <  1:244388/120‑1 (MQ=255)                                                                                                                                                                                                                                             agGGATACTGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAg                                                                                                                                     >  2:244388/1‑120 (MQ=255)                                                                                                                                                                                                                                             agGGATACTGTTACGATCGGAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGCGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACt  >  2:139562/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   TCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCCAGCGTTTGCTGCACGTTATCGACTTTCTCCGGCATCATTTCTATCTGGTAGATAGTGCGGTTGAGGGCCAGAGGGATACCGTTACGATCGTAGATAATGCCGCGGCTGGGCGCGATAGGCACCAGCTTAATGCGGTTTTCATTAGAGCGGGTCTGGTAGTCGGTAAAGCGAACAATTTGCAGATTATACAGGTTGGCGATAAGCACGCCGGTCAGCAGCAAAATCCCCAAAAAGGCGACCAGCGCCCGGCGCACAAACAGCGCGGACTCTGCCGTATAGTCGCGAAAAGAGTTCTGTAGTTTCATCCGCTGCGTTTTCTACT  >  NC_000913/667748‑668234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑