Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,807,825	C→T	S10S (AGC→AGT)	yniA →	putative kinase YniA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,825	0	C	T	100.0%	104.2 / NA	32	S10S (AGC→AGT)	yniA	putative kinase YniA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/12);  total (20/12)

GAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTAT  >  NC_000913/1807586‑1808071                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       gaagaaATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAgg                                                                                                                                                                                                                                             >  2:81012/1‑251 (MQ=255)    gaaATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTg                                                                                                                                                                                                                                           >  1:414173/1‑250 (MQ=255)                      tCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCg                                                                                                                                                                                                                        >  1:218904/1‑251 (MQ=255)                                    aaGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTg                                                                                                                                                                                                          >  1:317898/1‑251 (MQ=255)                                                  aaGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCAt                                                                                                                                                                                            <  1:9863/251‑1 (MQ=255)                                                        cTGACATCACCATCACGCCCTGGGAGTAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCTCATTCTTGCGCTAACCTTATGATCTGTCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAATTCCATGCCGcc                                                                                                                                                                                      >  2:23768/1‑250 (MQ=255)                                                           aaaTCACGCTCACGAACTGGGAGGAATGGAATAGCCACAGAAATTAAATGGCTTTTGGGCAATAATCAGTCTGTGGGGTGCGTTAGCCCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGAAATCATGAGGCAGACAATCAGTCGTCTTTTGAGTGAGCAGTTAGGCGAAGGCGAAATCGAACTGCGTAATGAATTGCCTGGCGGAGAAGTCCATGCCGCATgg                                                                                                                                                                                   >  2:25653/1‑251 (MQ=255)                                                                     cACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCt                                                                                                                                                                         >  2:7198/1‑251 (MQ=255)                                                                       cGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGATCTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTAt                                                                                                                                                                       <  1:118046/251‑1 (MQ=255)                                                                             gggAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGa                                                                                                                                                                 <  1:26555/251‑1 (MQ=255)                                                                                   aatggaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGAGGCGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGACAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAt                                                                                                                                                           >  1:394807/1‑251 (MQ=255)                                                                                       gaatAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGAttttt                                                                                                                                                       >  2:356782/1‑251 (MQ=255)                                                                                               aCAGCAAATAACTGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCTTCaa                                                                                                                                                >  2:112360/1‑250 (MQ=255)                                                                                                    aaataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCTAATGtga                                                                                                                                          <  1:320401/251‑1 (MQ=255)                                                                                                     aataaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGtgat                                                                                                                                         <  2:5401/251‑1 (MQ=255)                                                                                                        aaatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGtgatga                                                                                                                                       <  1:372055/250‑1 (MQ=255)                                                                                                               ttttGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAAc                                                                                                                               >  2:199567/1‑251 (MQ=255)                                                                                                               ttttGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTCGCGCTAACCGTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGaa                                                                                                                                <  2:142539/250‑1 (MQ=255)                                                                                                                 ttGGGCAATAATCCGTCTGAGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAACTGTGATGAAAGGGAACTg                                                                                                                             >  2:68401/1‑251 (MQ=255)                                                                                                                   gggCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTAGACCGCATTCTTGCGATAATCTTATGATCTGGAAGACAACATGGGAGAGACATCCTGTGGCAGGCAATCAGTAGTCTTTTGAGTGACCAGTTAGGTGAAGGCGAAATCGAACTGCGTACTGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATGTGCGCTATGCAGGAGATGATGAGTTCATAAATTGTGATGAACGGGAACTGCt                                                                                                                           >  2:175388/1‑251 (MQ=255)                                                                                                                          aatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGt                                                                                                                    >  1:233399/1‑251 (MQ=255)                                                                                                                                   gtggtgTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGTAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTTATGAAAGGGAACTGCTTCCCGGTTTTAccgcc                                                                                                           <  1:103462/251‑1 (MQ=255)                                                                                                                                   gtggtgTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAccgcc                                                                                                           <  1:81012/251‑1 (MQ=255)                                                                                                                                     ggtgTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTATTGAATTGCCTGGCGGAGAAGTCCATGCGGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTAccgccg                                                                                                          >  1:179032/1‑250 (MQ=255)                                                                                                                                            gTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGAc                                                                                                  <  2:275459/251‑1 (MQ=255)                                                                                                                                                              acCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTcg                                                                                >  1:231473/1‑251 (MQ=255)                                                                                                                                                                                      gATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTCTGAGTAAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCt                                                        >  2:29781/1‑251 (MQ=255)                                                                                                                                                                                                  aacaTGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGc                                             >  1:179207/1‑250 (MQ=255)                                                                                                                                                                                                       gggAGAGACAGCATGTGGCAGGCAATCGGGCGTCTTGTGAGTGAGCAGTTAGGATAAGGCGAAATCGAACTGCGTAATGAACTGCCGGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGGAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTg                                        <  2:211762/250‑1 (MQ=255)                                                                                                                                                                                                         gagagaCATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTg                                        >  2:324129/1‑248 (MQ=255)                                                                                                                                                                                                         gagagaCATCATGTGGCAGGCAATCAGTCGTCTTTTGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTg                                        <  1:324129/248‑1 (MQ=255)                                                                                                                                                                                                                                         ttttGAGTGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGAt     >  1:124193/1‑251 (MQ=255)                                                                                                                                                                                                                                            tgagtgagCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGACCTGCCTGCCGGAGCAGTCCATGCCGCATGGCCTTTGCGCTATGCAGGCCATGATTTTTTCGTCAAATGTGATGAAAGGAAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGCGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTAt  >  2:268241/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       GAAGAAATTAGAGAACTGGTTTCGGGGCTTACCCAAAGTGCAACCGGCAAAGATCCTGAAATCACCATCACGACCTGGGAGGAATGGAATAGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAGCAGTTAGGTGAAGGCGAAATCGAACTGCGTAATGAACTGCCTGGCGGAGAAGTCCATGCCGCATGGCATTTGCGCTATGCAGGACATGATTTTTTCGTCAAATGTGATGAAAGGGAACTGCTTCCCGGTTTTACCGCCGAAGCCGACCAACTGGAGTTACTGTCGCGTAGTAAAACCGTCACCGTGCCTAAGGTTTGGGCAGTTGGCGCTGACCGTGACTACAGTTTTCTGGTGATGGATTAT  >  NC_000913/1807586‑1808071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑