Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,982,189	G→A	L67F (CTT→TTT)	otsB ←	trehalose‑6‑phosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,982,189	0	G	A	100.0%	196.1 / NA	55	L67F (CTT→TTT)	otsB	trehalose‑6‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/32);  total (23/32)

GCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCT  >  NC_000913/1981946‑1982434                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gcgcTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTccac                                                                                                                                                                                                                                                 <  2:223174/250‑1 (MQ=255)      aaTGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTg                                                                                                                                                                                                                                            >  2:205144/1‑250 (MQ=255)           cATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGcc                                                                                                                                                                                                                                      <  1:410566/251‑1 (MQ=255)           cATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCAGCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAgcgc                                                                                                                                                                                                                                       <  1:392588/250‑1 (MQ=255)                  gCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAGCGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGata                                                                                                                                                                                                                                <  1:332448/250‑1 (MQ=255)                  gCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGatat                                                                                                                                                                                                                               <  1:157821/251‑1 (MQ=255)                        tCATGCTGCGTAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATcaatgc                                                                                                                                                                                                                         >  2:271767/1‑251 (MQ=255)                                   aGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCAt                                                                                                                                                                                                              >  2:364779/1‑251 (MQ=255)                                                      gcAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAg                                                                                                                                                                                           <  1:42336/251‑1 (MQ=255)                                                           aGCCATCCCCTTCGCCGCCAGCGCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCTCCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTa                                                                                                                                                                                      <  2:193252/251‑1 (MQ=255)                                                                         ccTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAat                                                                                                                                                                         >  1:208356/1‑250 (MQ=255)                                                                                 tCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCACCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGAGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAgg                                                                                                                                                                >  1:54293/1‑251 (MQ=255)                                                                                      gcCGGGATACTGAGCGATGACTGTATGCAGTTGTACCCTAATATCACGCGCAATCGCACCCGGCAGATGAACGATATGTGTTTTACCATTAATGTCACGGCGATCCGACACATGCACGCCAGCTAACGGGAAGCGATAAGGCTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCACTGCCACTGAACCATCACTTGCGCTTGCCAGTAGCTGCAATCCTTGCAGAATCTTGTcacgcacg                                                                                                                                                            >  1:177214/1‑250 (MQ=255)                                                                                       ccGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTTCCATTGATGTCACGGCGCGCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCacgac                                                                                                                                                          <  2:140331/251‑1 (MQ=255)                                                                                              aCTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTg                                                                                                                                                    >  2:314786/1‑250 (MQ=255)                                                                                                tGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATc                                                                                                                                                 >  1:99552/1‑251 (MQ=255)                                                                                                 gAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCTCGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTATTCCTTGCAGAATATTGTCAGGCACGACGACCTGATc                                                                                                                                                 <  2:227763/250‑1 (MQ=255)                                                                                                      aTGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATCTGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTACACCATTGAGCGCCCTGATATAAATGCCAATGCACCATCACTTGCGGTTGCCCGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATg                                                                                                                                           >  1:57496/1‑251 (MQ=255)                                                                                                        gACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGc                                                                                                                                          <  2:328792/250‑1 (MQ=255)                                                                                                            gTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGttt                                                                                                                                     >  1:347929/1‑251 (MQ=255)                                                                                                            gTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGtt                                                                                                                                      <  1:158991/250‑1 (MQ=255)                                                                                                                   gTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTcc                                                                                                                              >  1:143368/1‑251 (MQ=255)                                                                                                                        aCGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAg                                                                                                                         <  1:205144/251‑1 (MQ=255)                                                                                                                         cGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGc                                                                                                                        <  1:96547/251‑1 (MQ=255)                                                                                                                                               aTCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAAcc                                                                                                  >  1:134410/1‑251 (MQ=255)                                                                                                                                                    gCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCACGATCAAAAAACCAGGc                                                                                              >  2:229566/1‑250 (MQ=255)                                                                                                                                                    gCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACATGATCGGGATGCGGTTTGCTTTCCGCCAGCGTTCCATAAAGATCAAAAAACCAGGc                                                                                              >  1:249820/1‑250 (MQ=255)                                                                                                                                                     cAGATGAACGAGATGTGTTTTACCATTCATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCGAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCAAGCGTTCCATCAAGATCAAAAAACCAGGCat                                                                                            <  1:280993/251‑1 (MQ=255)                                                                                                                                                      ataTGAACGATATGTGTTTTACCATTTGTGTCACGGCGCTCCGCCCCATGCACGATAGCTAACAGGAGGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg                                                                                                                                                                            >  2:383976/3‑170 (MQ=255)                                                                                                                                                      aGATGAACGGTATGTGTTTTACCATTGATGTCACGGCGCTCCGTCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAg                                                                                                                                                                            <  1:383976/170‑1 (MQ=255)                                                                                                                                                       gATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCatat                                                                                          <  2:332532/251‑1 (MQ=255)                                                                                                                                                          taaCGATATGTGTTTTACCATTTATGTCACGTCGCTCCGCCCCATGCACGCCCTCTATCGTGATGCTATAAGGTTTTGCCAGTGCGTCCAACTCCACCATTTAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGTTTGCCAGTAGCTGTAGTCCTTTCAGAATATTGTCAGGCACGACGTCCTGATCGGTATTCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATAttt                                                                                        <  2:340539/249‑1 (MQ=255)                                                                                                                                                          gAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTACCGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAGTGCACCATCACTTGCGGTTGCAAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCATCGTTCCATCAAGATCAAAAAACAAGGCATATTTc                                                                                       >  2:194682/1‑251 (MQ=255)                                                                                                                                                          gAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTc                                                                                       <  1:74734/251‑1 (MQ=255)                                                                                                                                                             cGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCAATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCATGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTcgcg                                                                                    <  1:269089/251‑1 (MQ=255)                                                                                                                                                                     gtTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCaaaaaa                                                                                                    <  2:278466/227‑1 (MQ=255)                                                                                                                                                                     gtTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCaaaaaa                                                                                                    >  1:278466/1‑227 (MQ=255)                                                                                                                                                                        ttACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAg                                                                          <  1:164270/250‑1 (MQ=255)                                                                                                                                                                                     cACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAAc                                                            <  1:332080/251‑1 (MQ=255)                                                                                                                                                                                        ggCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGt                                                         >  1:277184/1‑251 (MQ=255)                                                                                                                                                                                              ccGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTg                                                     <  1:314786/249‑1 (MQ=255)                                                                                                                                                                                               cGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCa                                                  <  2:134410/251‑1 (MQ=255)                                                                                                                                                                                                gCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCACTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAATATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCAc                                                 >  1:1033/1‑251 (MQ=255)                                                                                                                                                                                                   ccATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCg                                              >  1:340433/1‑251 (MQ=255)                                                                                                                                                                                                     aTGCACGGCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAAGGCACCATCACATGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCGCGACGACCTGATCGGGATGGGTTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAGACCAGGGATATTGCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGt                                            <  1:278516/251‑1 (MQ=255)                                                                                                                                                                                                           gCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTctcct                                      <  1:364779/251‑1 (MQ=255)                                                                                                                                                                                                                  aaCGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTtctc                               >  1:109319/1‑251 (MQ=255)                                                                                                                                                                                                                  aaCGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTtctc                               <  2:138905/251‑1 (MQ=255)                                                                                                                                                                                                                               aaGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATtgt                   <  2:277184/250‑1 (MQ=255)                                                                                                                                                                                                                               aaGGTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAAAGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATtgtg                  >  2:295675/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTg               <  2:340433/251‑1 (MQ=255)                                                                                                                                                                                                                                  gTTTTGCCAGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTg               <  2:208356/251‑1 (MQ=255)                                                                                                                                                                                                                                          aGTGCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAAc       >  1:22995/1‑251 (MQ=255)                                                                                                                                                                                                                                             gCGTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAAcgcg    <  2:261414/251‑1 (MQ=255)                                                                                                                                                                                                                                               gTCAAACTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCt  <  2:52986/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GCGCTAATGTCATTAATGCGTCTTCATGCTGCGGAGCCTGACGATAATGCAGCGCAAAAGCCATCCCTTTCGCCTCCAGCTCCGCGCCGGGATACTGAGCGATGACTGTATGCAGTTGCACGCTAATATCACGCGCAATCGCATCCGGCAGATGAACGATATGTGTTTTACCATTGATGTCACGGCGCTCCGCCCCATGCACGCCCGCTAACGGGAAGCGATAAGGTTTTGCCAGTGCGTCAAGCTCCACCATTGAGCGCCCTGATATCAATGCCAATGCACCATCACTTGCGGTTGCCAGTAGCTGTAGTCCTTGCAGAATATTGTCAGGCACGACGACCTGATCGGGATGCGGTTTGATTTCCGCCAGCGTTCCATCAAGATCAAAAAACCAGGCATATTTCGCGGATAGTTCAGGGGTTTCGGTTAACGGTTCTGTCACCCGGTTCTCCTCCTTCTCTTTTCTTATTGTGTTGCGCAGAACGCGCT  >  NC_000913/1981946‑1982434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑