Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,820,949	C→T	G89E (GGA→GAA)	chbC ←	N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,820,949	0	C	T	100.0%	73.6 / NA	23	G89E (GGA→GAA)	chbC	N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/15);  total (8/15)

GCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCGCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTCCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGAAAAACGTTGTTAATTAATACAAACATGGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAA  >  NC_000913/1820708‑1821173                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 gcggcgGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCGCCCCACCTAACCAGTTTGCCCCAACCGCATAGGCCTCACCGACCCTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTtatat                                                                                                                                                                                                                          <  1:917/250‑1 (MQ=255)      ggACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCGCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTg                                                                                                                                                                                                                    <  2:403959/251‑1 (MQ=255)               agtaaaaCATTCCAGCCCCCACCAGGCCAATAAAAACCCCGGAGATGATATTCGCCCCACCTACCCAGTTTCCCCCAACCGCAGAGGCCTACCCGACACTATATGGGGTGACGGTCATAAATGCTGAAACGGAAAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATa                                                                                                                                                                                                           <  2:133629/247‑1 (MQ=255)                 gTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATCCGCCCCCACTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGACACGGTCATAACTGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAATACATTATTCCTAATGTTTCGTTATATCCGTTGCCGCAAATAcc                                                                                                                                                                                                         >  1:260957/1‑251 (MQ=255)                 gTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATCCGCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATAcc                                                                                                                                                                                                         <  1:122269/251‑1 (MQ=255)                       aTTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCGCCCCCCCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATTCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAg                                                                                                                                                                                                   <  1:379444/251‑1 (MQ=255)                                 ccaccaGCCCAATAATAATCCCGGAGATGATATTCCCCCCCCCTAACCAGTTTGCACCTACCGCATAGGCCTCACCTACACTATATGGGGTGCCGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATTCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAgt                                                                                                                                                                                         <  1:60374/251‑1 (MQ=255)                                    ccaGGCCAATAATAATCCCGGAGATGATATTCGCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGtt                                                                                                                                                                                      <  1:5085/251‑1 (MQ=255)                                                 aatCCCGGAGATGATATTCGCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAgg                                                                                                                                                                         >  1:401941/1‑251 (MQ=255)                                                                    gCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTa                                                                                                                                                       >  1:406482/1‑250 (MQ=255)                                                                                 agtttaccccAACCGCATAGGCCTCCCCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAAc                                                                                                                                         <  1:196910/243‑1 (MQ=255)                                                                                               gCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTTCCAGCGCCATGCCAATAAAGACCGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGa                                                                                                                                                   <  2:74585/227‑1 (MQ=255)                                                                                               gCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGa                                                                                                                                                   >  1:74585/1‑227 (MQ=255)                                                                                                cATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAg                                                                                                                          <  1:336712/251‑1 (MQ=255)                                                                                                       cTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGa                                                                                                                    >  1:73156/1‑250 (MQ=255)                                                                                                                         ggTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGATCGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATAcc                                                                                                                                                         <  1:161381/195‑1 (MQ=255)                                                                                                                         ggTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCACTACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATAcc                                                                                                                                                         >  2:161381/1‑195 (MQ=255)                                                                                                                                                                                                           aTAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGAAAAACGTTGTTAATTAATACAAACATGGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTAt               <  1:98055/251‑1 (MQ=255)                                                                                                                                                                                                            aaaaGAACGGTGCCATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGAAAAACGTTGTTAATTAATACAAACATGGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCAGTAACGGGTGGCTGCTTTCCTAtt              <  2:58126/250‑1 (MQ=255)                                                                                                                                                                                                              aaGAACGGTACCATTGAAGACATTATTCCTAATGTTTAGTTATATACGTTGCCGCCAATACCGTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGAAAAACTTTGTTAATTAATACAAACATGGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGACATTTTTGATTGCATTAACGTGTGACTGATTTCATAtttt            >  2:85685/1‑251 (MQ=255)                                                                                                                                                                                                                        ccATTAAAGACATTATTCCTAATGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGAAAAACGTTGTTAATTAATACAAACATGGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCaaaa  <  2:73156/251‑1 (MQ=255)                                                                                                                                                                                                                                              tGTTTCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTACGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGAAAAACGTTGTTAATTAATACAAACATGGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTg                                            >  1:226480/1‑187 (MQ=255)                                                                                                                                                                                                                                              tGTTTCGTTATATACGTTGCCGCCAATACCATTCAGACCATTAAGTGTTTCAATGGGTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGAAAAACGTTGTTAATTAATACAAACATGGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTg                                            <  2:226480/187‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 GCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCGCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCAATAAAGAACGGTGCCATTAAAGACATTATTCCTAATGTTCCGTTATATACGTTGCCGCCAATACCTTTCAGACCATTAAGTGTTTCAATGGTTGAGGCGTCGAGGCGAATACCTAAGGAATAAAAAAACGACCCCTCCCCAAAGCTTAGAAAAACGTTGTTAATTAATACAAACATGGCCCCCGCAAGGGTTAACGGCATTAAGCGAATAAAGCCATTTTTGATTGCATTAACGTGTGGCTGCTTTCCTATTTTAACTGCAAAA  >  NC_000913/1820708‑1821173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑