Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,871,577	C→T	E52K (GAA→AAA)	dgoT ←	D‑galactonate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,871,577	0	C	T	97.1%	102.2 / NA	34	E52K (GAA→AAA)	dgoT	D‑galactonate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (14/19);  minor base G (0/1);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTCCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTT  >  NC_000913/3871334‑3871818                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  aCCAGCTGGTCACAATCAGGTTATTGGCCGGGGAAGAAGGCGCTTCGAAAATACCGGTTATAGCGAGAAGACCAATTAATGACATTACGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACAAGCCAAATATCGCAATAGAATAAGTAACGCGAGAACCTACGCGATCTAAAAACCCACCGCCGGGGATCTGACATAGCGTATAAAGACAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTg                                                                                                                                                                                                                                            >  2:149342/1‑251 (MQ=255)         gTCACCATCCGTTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCACACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGTGATCTAAAACCCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGcc                                                                                                                                                                                                                                    <  1:160426/251‑1 (MQ=255)         gTCACCATCCGGGTAGTGGGCGGGAAAGCAGGCGCTTCGAAAATCCCGTTGATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGACCCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGcc                                                                                                                                                                                                                                    <  2:31655/251‑1 (MQ=255)              cATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATACCATTAACCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAAct                                                                                                                                                                                                                               <  1:90422/251‑1 (MQ=255)               aTCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATAAATGACATTAAGCCCGTGGCAAAGCCCTGAAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAActc                                                                                                                                                                                                                              <  1:127493/251‑1 (MQ=255)               aTCCGGTTATTGGACGGGAAAGCAGGCGATTCGAAAATACAGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCAGTGGCAAATCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAAAAGCCGGGGATCTGACATAGAGTATACAATCAGGCGAAGACCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAActc                                                                                                                                                                                                                              >  2:291747/1‑251 (MQ=255)                     ttATTGGTCGGAAAAGCAGGCGCTTCGAAAATACCGATTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCt                                                                                                                                                                                                                         <  2:2720/250‑1 (MQ=255)                       aTTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACAAATTAATGAAATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATAGCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGa                                                                                                                                                                                                                       <  1:417037/250‑1 (MQ=255)                          ggTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGCCCAGCCAAATATCGCAATAAAATAAGTCACGCGATAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAatat                                                                                                                                                                                                                   <  1:391511/251‑1 (MQ=255)                            tCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACAAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGACCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATgg                                                                                                                                                                                                                 >  2:320076/1‑251 (MQ=255)                              gggAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGc                                                                                                                                                                                                               >  1:27353/1‑251 (MQ=255)                               ggAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCg                                                                                                                                                                                                              <  2:212788/251‑1 (MQ=255)                                      aGGCGCTTCAAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAAAAAAGCCGCACCTGACCAGCCAAATATCCCAATAAAATAAGTCACGCAAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGTCATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCa                                                                                                                                                                                                       <  2:116280/251‑1 (MQ=255)                                        gcgcTTCGAAAATCCCTGTTTTCTCGCCCAGACCATTTCATCACATTAACCCCGTGGCAATGCCCTGGAATAAAGTCCCCCCTGCCCAGCCATATATCTCAATAAAATAAGTCACGCGTGACCCTACCCGATCTAAAAACCAACCGCCGGGTATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAAGGCCGAACTCTTCCTTAATATGGGCGGAAGCCACg                                                                                                                                                                                                     <  1:154766/251‑1 (MQ=255)                                                         gTTATCGCGCGCAGACCAATTAAGGACATTAAGCCCGTGCCAAAGCCCTGGAATAAAGTCGCCCCGGCCCAGCCAAATATCGCAACAAAATAAGCCCCGCCAGAACCTACGCGATCTAAAACCAAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGTGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCCCGGCCACGTTGGCGCGGTc                                                                                                                                                                                    <  2:91267/251‑1 (MQ=255)                                                          ttATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCg                                                                                                                                                                                   >  1:345885/1‑251 (MQ=255)                                                            cccgcgcgAAGACCAATTAAAAAAATTAATCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACGGACAAGCAAACTAACGAAATAATATAAGGCACGCGAGAACATACGCGAGCTAAAAACCAACCGCCGGGGATCTGAAATAGCGTATAAAGCCAGGAGAAGGCCGAAAATACATAGCCCATTTGCGCTTTGGTAATGCCGAACTCTTCCGGAATATGGGCGGAAGCCACGGCGAGGTTAGCGCGGTCGaa                                                                                                                                                                                 <  2:139004/249‑2 (MQ=255)                                                                 gcgcAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGACACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAAc                                                                                                                                                                            >  2:78941/1‑251 (MQ=255)                                                                          aaTTAATGACATTAAGCCCGAGGCAAACCCCTGGAATAACGTCGCCAATGAACAGAAACATATCGCAATAAAATACGTCACGCGAGCACCTACGCGATCTAAAAACAAACCGCCGGGGATCTGACATAGCGTATACAGCCAGGCGAAGACCGAAAATAAATAGCCCATTTTCGCTTTGGTAATGCCGACCTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACt                                                                                                                                                                    >  2:36782/1‑250 (MQ=255)                                                                           aTTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGATGGCGCGGCCGACATAACAAATGACTAc                                                                                                                                                                  >  2:96325/1‑251 (MQ=255)                                                                              aaTGACATTAAGCCCGGGGCAAAGCCCGGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGt                                                                                                                                                               <  1:228194/251‑1 (MQ=255)                                                                                                   aaGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCa                                                                                                                                                                                                 >  2:217268/1‑196 (MQ=255)                                                                                                   aaGCCCTGGAATAAAGTCGCCACGGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCa                                                                                                                                                                                                 <  1:217268/196‑1 (MQ=255)                                                                                                           gaagaaaGTCGCCACTGACCAACCAAATATCGCAATAAAATAAGTCCCGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCGGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAAc                                                                                                                                  <  1:276502/247‑1 (MQ=255)                                                                                                                 aGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGAc                                                                                                                            >  1:219324/1‑251 (MQ=255)                                                                                                                                                            cgAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCtt                                                                                 >  1:215359/1‑251 (MQ=255)                                                                                                                                                                                 aaccacccGCCTGGGATCTGACATAGCGTATAAAGTCAGGCGAAGGACGAAAATACATAGCCGATTTTCGCTTTGGTAATGCCGAACTCTTCCGGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATATCGAGCTTTCTCCAGATTTTGGGCATAGc                                                            <  1:291747/251‑1 (MQ=255)                                                                                                                                                                                    caaccGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAgaga                                                          >  1:128949/1‑247 (MQ=255)                                                                                                                                                                                                                          gAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCGGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGcc                                                                                                                               <  1:250857/143‑1 (MQ=255)                                                                                                                                                                                                                          gAAGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCGGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAAAGcc                                                                                                                               >  2:250857/1‑143 (MQ=255)                                                                                                                                                                                                                            aGGCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAttt                 <  1:245653/251‑1 (MQ=255)                                                                                                                                                                                                                              gCCGAAAATACATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAttttt               >  1:82541/1‑251 (MQ=255)                                                                                                                                                                                                                                       aCATAGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTg      <  2:325353/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGCCCATTTTCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTGACAGAGGGTTATATTTTTGTATTGCTGtttt  <  1:229142/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  ACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAAATAAGTCACGCGAGAACCTACGCGATCTAAAAACCAACCGCCGGGGATCTGACATAGCGTATAAAGCCAGGCGAAGGCCGAAAATACATAGCCCATTTCCGCTTTGGTAATGCCGAACTCTTCCTGAATATGGGCGGAAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTT  >  NC_000913/3871334‑3871818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑