Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,916,210	C→T	intergenic (‑82/‑48)	msrC ← / → letA	free methionine‑(R)‑sulfoxide reductase/intermembrane transport protein LetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,916,210	0	C	T	100.0%	44.1 / NA	14	intergenic (‑82/‑48)	msrC/letA	free methionine‑(R)‑sulfoxide reductase/intermembrane transport protein LetA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/7);  total (7/7)

CAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCCCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTCATGGCTCTTAACACACCACAAATTACGCCGACAAAAAAGATAACAGTGAGGGCAATCGGCGAGGAACTGCCGCGTGGTGATTACCAACGTTGCCCGCAATGTGACATGCTGTTTAGCCTGCCCGAGATAAATTCTCATCAAAGTGCCTATTGTCCGCGCTGTCAGGCAAAAATTCGTGACG  >  NC_000913/1915964‑1916438                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     cAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCGCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGGCTTACCTGGATCTGCCAGCCGATTGAAATAAGCATTAAAGGCGGTAATGCTCAAGATCATTCTcatc                                                                                                                                                                                                                                  <  2:260244/251‑1 (MQ=255)                  gCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATg                                                                                                                                                                                                                 <  2:301891/251‑1 (MQ=255)                        aaaaaCCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAAtt                                                                                                                                                                                                           <  1:202100/251‑1 (MQ=255)                          aacccTGCCCAGTTTATGTCAGTTAGACGCTCATATAACAACGCACTGGTGTTCGCAATCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCt                                                                                                                                                                                                         <  2:305863/248‑1 (MQ=255)                                 cccAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTAc                                                                                                                                                                                                   >  1:108028/1‑250 (MQ=255)                                         aTGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCAc                                                                                                                                                                                          >  1:273601/1‑251 (MQ=255)                                             cAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTc                                                                                                                                                                                       >  2:293940/1‑250 (MQ=255)                                                                       cTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGGGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTCATGGCTCTTAACACACCACAAATTACg                                                                                                                                                            >  2:399954/1‑251 (MQ=255)                                                                                                                                     aTTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATGCTTATTACATTTGGCACGTCACGGCTCTGAACACACCACAAATTGCGCCTACAAAAAAGATAACAGTGAGGGCAATCGGCGAGGAACTGCCGCGTGCTGATTACCAACg                                                                                              <  2:29607/251‑1 (MQ=255)                                                                                                                                                                   cATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTCATGGCTCTTAACACACCACAAATTACGCCGACAAAAAAGATAACAGTGAGGGCAATCGGCGAGGAACTGCCGCGTGGTGATTACCAACGTTGCCCGCAATGTGACATGCTGTTTAGCCt                                                                >  1:398860/1‑251 (MQ=255)                                                                                                                                                                                           taccctgATCTGCCAGCCGATTAAAATAAGCATTAAATGCGTTAATTCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTCCTGGCTCTTAACACCCCACAAAGTACGCCGACAAAAAAGACAACAGTGTGGGCAATCGGCGAGGAACTGCCGCGTGGTGATTACCAACTTTGTCCGCAATGTCTCATGCTGTTTAGGCTGCGGGTGATGAATTCtcatca                                           <  2:267419/242‑1 (MQ=255)                                                                                                                                                                                           tACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTCATGGCTCTTAACACACCACAAATTACGCCGACAAAAAAGATAACAGTGAGGGCAATCGGCGAGGAACTGCCGCGTGGTGATTACCAACGTTGCCCGCAATGTGACATGCTGTTTAGCCTGCCCGAGATAAATTCTCATCaaa                                         <  1:27591/250‑1 (MQ=255)                                                                                                                                                                                                      gCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTCATGGCTCTTAACACACCACAAATTACGCCGACAAAAAAGATAACAGTGAGGGCAATCGGCGAGGAACTGCCGCGTGGTGATTACCAACGTTGCCCGCAATGTGACATGCTGTTTAGCCTGCCCGAGATAAATTCTCATCAAAGTGCCTATTGTc                             >  1:85916/1‑251 (MQ=255)                                                                                                                                                                                                                                 gCGTTAATGCTCAAGATCATTCTCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTCATGGCTCTTAACACACCACAAATTACGCCGACAAAAAAGATAACAGTGAGGGCAATCGGCGAGGAACTGCCGCGTGGTGATTACCAACGTTGCCCGCAATGTGACATGCTGTTTAGCCTGCCCGAGATAAATTCTCATCAAAGTGCCTATTGTCCGCGCTGTCAGGCAAAAATTCGTGACg  >  2:20661/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     CAGTGTATCGTCCTCAAGCAAATAAAAACCTGCCCAGTTTATGTCAGTGAGACGCTCATATAACAACGCACTGGTGTTCGCAAGCGTTGCCAGAAAACTGGTTTCTCCCGCCATCAGCGCGTTAAAGTCGCGATTTAAATCCGCGTAAAATTCTGTTTTGTTCATTATATAATCACTTGGTTGTCTTACCTGGATCTGCCAGCCTATTAAAATAAGCATTAAATGCGTTAATGCTCAAGATCATTCCCATCATGGGTTAAGATTAATGTTAATTCTTATTACATTTGGCACGTCATGGCTCTTAACACACCACAAATTACGCCGACAAAAAAGATAACAGTGAGGGCAATCGGCGAGGAACTGCCGCGTGGTGATTACCAACGTTGCCCGCAATGTGACATGCTGTTTAGCCTGCCCGAGATAAATTCTCATCAAAGTGCCTATTGTCCGCGCTGTCAGGCAAAAATTCGTGACG  >  NC_000913/1915964‑1916438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑