Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	73.0 / NA	23	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/12);  total (11/12)

ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652087‑1652574                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    aTGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGCGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCCTTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCagag                                                                                                                                                                                                                                               <  1:179674/251‑1 (MQ=255)                                           gtgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGtttt                                                                                                                                                                                                       <  2:142732/249‑1 (MQ=255)                                           gggtgCACTTGGATGGGAGGACATAGATCTGAAGAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTc                                                                                                                                                                                                     <  2:210540/249‑1 (MQ=255)                                                                    gATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATTGGGGCATTCATTTTATTAAGACAGCAATAGGCGCCCTCCGGAGTCAGATGACATTAAAGAGACTGAGGAAAAAGCATATCATTGATGATCACCTCAGAGAGGATGGCCGAACAGAAAAACAAAAAAGCACCTTTGTTTTTTAAACTTAAGTGACACCGAGAGTaa                                                                                                                                                                             >  2:272115/1‑250 (MQ=255)                                                                             aaTGGAACAATGATGATCAGGAGAAATTTCGCAAAAGACCGTTTCACGGTACCAAAAACTCAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATgg                                                                                                                                                                   <  2:191065/251‑1 (MQ=255)                                                                                                                aGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCACTCCTCTTCTTAAGCCAGCAATCGACGCTCTACGGAGGCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGAATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTTAACCTGAAGGGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGAtgg                                                                                                                                >  1:46297/1‑250 (MQ=255)                                                                                                                                                  aCCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGcc                                                                                               >  1:82761/1‑250 (MQ=255)                                                                                                                                                           gTCATTCATCTTATTAAGCCATCAATCGACGCTCTGCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATTCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGTACGCAGCGATAAAACGTGCCGGTCTCCGCCATCGAAAATc                                                                                     <  2:284159/251‑1 (MQ=255)                                                                                                                                                             cattcatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAACAATTATGGTGACCATTTTACCGTTGGCTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAACTCat                                                                                   >  1:186403/1‑251 (MQ=255)                                                                                                                                                               ttcatCTTATTACGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAACTGCACCTTTGTTTTTCAACCTGACGTGACAGCGAGAGAAAAAAATTATGGTGACCATTTTACAGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGAGCCGGACTCCGCCATCGAAAATCatat                                                                                 >  2:41272/1‑251 (MQ=255)                                                                                                                                                                tcatCTCATTCAGCTAGCAATCGACGTTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAAAGCATCTCATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGTTGTGGTACGCAGCGATAAAACGAGCAGGACTCCGACATCGATAAtaaaatc                                                                                >  2:273106/1‑245 (MQ=255)                                                                                                                                                                 catCTTATTAAGCCAGCAATCGACGCGCGCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGTGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCGTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGTCCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCa                                                                               <  2:166127/251‑1 (MQ=255)                                                                                                                                                                       attaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGGTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGACGGACTCCGCCATCGAAAATCATATCAGTCga                                                                          <  2:81649/250‑1 (MQ=255)                                                                                                                                                                           aGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACAt                                                                     >  2:230750/1‑251 (MQ=255)                                                                                                                                                                            gCCAGCAATCGACGATCTCCGGAGACAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACAt                                                                     >  2:241981/1‑250 (MQ=255)                                                                                                                                                                                               cGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCTCCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATTCTTATGCCTGCTGGTCGCt                                                 <  2:14414/251‑1 (MQ=255)                                                                                                                                                                                                  aGTCAGATGAAATCAACGAGCCTGAGCAACGAGCAGACCATTGATGTTCACCTCAACGCGTATGGCATAACAGAAAAACAAACATGCACCTTTGTTTTTCAACCTGAAATGTCAGCGAGAGAAAAAAATTATGGTGACCATTTTCCCGTTGACTCAATAAGGCAGATGTGTAACGCAGCGTTAAAACGTGCCGGACTCCGACATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGTTCGCttac                                              >  2:74015/1‑248 (MQ=255)                                                                                                                                                                                                                         gAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGGCTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCTGACTCCGCCATCGAAAATCATATCAGTCGAGACATACGTATGCGTGCTGGTCGCTGACAGCTGGTGCTAGCCCGGCAttt                        <  2:186403/250‑1 (MQ=255)                                                                                                                                                                                                                            aaaaGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAAAAGAAAAAAAAAAATGAACCTTTGTTTTTCAACCTGAAGAGTAAGCGAGAGTAAAAAATTATGGTAACCATTTTAACGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCAGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAACTGGTGCTAACCCGGCATTtat                      >  1:404806/2‑249 (MQ=255)                                                                                                                                                                                                                                 agCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAAc               >  1:12468/1‑251 (MQ=255)                                                                                                                                                                                                                                          attaTGTTCACCTCAAAGAGTATGGCAAAGCAGAAAAACAAAAATGCACCTTTGTTATTCAACCTGTAGTGTCAGCGACAGGAAAAAATTATGGTGATCATTTTACCGTTGACTCAAGAAGGCAGATGTGGGACGCAGCGATAAAACGTGACAGAGTCCACCATCGACAATCATATCAGTCGAGACATACTTATGCCTACTGGTCGTTGACAGATGGTGCTACCCCGGCCTTGATAGCTAACCAGATGGGc      <  2:272483/248‑1 (MQ=255)                                                                                                                                                                                                                                            gATGTTCACCTCAGAGAGTATGTCAGAACAGAAAAACAAAAATGCACCTTTGGTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGcc     <  2:78644/250‑1 (MQ=255)                                                                                                                                                                                                                                               gTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATg  <  2:407959/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    ATGGTGTGTTTCTGTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATG  >  NC_000913/1652087‑1652574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑