Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,234	C→T	H216H (CAC→CAT)	ydiF →	putative acetate‑CoA transferase YdiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,234	0	C	T	100.0%	62.2 / NA	18	H216H (CAC→CAT)	ydiF	putative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

GTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGA  >  NC_000913/1775987‑1776479                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTAATTCGCGCTACCACCTGCGACAGCGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCACTGGTTATTGCCCAGGCGGTGCATAAt                                                                                                                                                                                                                                                     >  2:125627/1‑251 (MQ=255)                  ggCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTCTTTt‑gat                                                                                                                                                                                                                                   >  2:99511/1‑251 (MQ=255)                          gACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAACGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTCTtgttgtggtggagg                                                                                                                                                                                                                            >  2:287655/1‑240 (MQ=255)                                                           aCTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGGATTGT‑GATGATTCAGGGGCAGGAAAAGGGTAAGaaaaaccagctgcagc                                                                                                                                                                                          >  1:220352/1‑238 (MQ=255)                                                                  gaaGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCAGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                                                                                                                                                                   <  2:354826/251‑1 (MQ=255)                                                                           aaaGAAGCCCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GGTGGTGCAGGTGCAGAAAAATGTTAAGAAAGCCACGCGTCATCCTAAATCTGTCCGTAt                                                                                                                                                                          >  1:320850/1‑251 (MQ=255)                                                                                                              cAAAGAATAACTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATc                                                                                                                                       <  2:205245/251‑1 (MQ=255)                                                                                                                aaGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCg                                                                                                                                     <  1:414461/251‑1 (MQ=255)                                                                                                                         tctATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGCATTTTTGTTG‑TGCAGGTGTAGAAAATGTTTATAAAAGCCACCCTTGATCCTAAATTTGTCCGTATTCCGGGTTTTTTTGGGGGTATTTTGGTGGTCGGTcctggttcaacc                                                                                                                            >  2:261516/1‑241 (MQ=255)                                                                                                                                            gcCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCAc                                                                                                         <  1:341540/251‑1 (MQ=255)                                                                                                                                                      gCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTTT‑GGTTATTCAGGGGCGGGAAATGGTTAAAAAAACCACGCTCCATCCTCAATATGTTCGTATTCCGGGTTATTTTGGGGGTATTTTGGGGGTGCCTCCGGATCAAAACCCACTTTATGGCgggggcacgcgttaccc                                                                                               >  2:145780/1‑236 (MQ=255)                                                                                                                                                                                  gTGAAGTCTACGCCACTTTTGAAGATGAGGTGATGTGTCTCGACGCATTGGTTATTGCACAGGCGGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCCTCCTAAATCTGTCCGTATTTCTGGTTATCTTGTGGATATTGTGGTGGTCGATCCGGATCAAACCCATCTGTATGGCGGTGCACCGGTTAACCGCTGTAGTTCTGGTGACTTCACCCTtgat                                                                   <  2:48191/251‑1 (MQ=255)                                                                                                                                                                                    gAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GATTATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTtgatga                                                                 <  2:276579/251‑1 (MQ=255)                                                                                                                                                                                      aGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACa                                                               <  1:99511/251‑1 (MQ=255)                                                                                                                                                                                                      gAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCt                                               <  1:145780/251‑1 (MQ=255)                                                                                                                                                                                                                               cATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGtt                      <  2:358548/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggTTATTGCCCAGGCGGTGCATAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCGCCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTgcgc                  <  2:153214/251‑1 (MQ=255)                                                                                                                                                                                                                                                   gTGCATAATAACGGCGGTATTGT‑GATGAAGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTAACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGa  <  1:218302/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGT‑GATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGA  >  NC_000913/1775987‑1776479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑