Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,810,624	C→T	I138I (ATC→ATT)	ydjM →	inner membrane protein YdjM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,810,624	0	C	T	100.0%	81.7 / NA	24	I138I (ATC→ATT)	ydjM	inner membrane protein YdjM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/15);  total (9/15)

CAAAACCGATCGCCCGCGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGT  >  NC_000913/1810395‑1810869                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cAAAACCGATCGCCCGCGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGcaac                                                                                                                                                                                                                                  >  1:32568/1‑251 (MQ=255)        gATCGCCCGCGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTgg                                                                                                                                                                                                                           >  2:304662/1‑251 (MQ=255)        gATCGCCCGCGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTgg                                                                                                                                                                                                                           >  1:377602/1‑251 (MQ=255)         atcccccGGGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCACCCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACCCCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGa                                                                                                                                                                                                                          <  1:172768/247‑1 (MQ=255)         aTCGCCCGCGCTTTTGGTCATCGGGTTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCACCCTTTTACCTTAAGGTTCCGGAAGGCTGTTTCATTCCGGTTTATGCGCTACAAGGAATGGTGCTGTTTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTGTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGa                                                                                                                                                                                                                          <  1:170422/251‑1 (MQ=255)            ccccGCGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACg                                                                                                                                                                                                                       <  1:380425/250‑1 (MQ=255)                               gTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCatt                                                                                                                                                                                                     >  1:76105/1‑250 (MQ=255)                                   ttttACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCCTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTAtttt                                                                                                                                                                                                >  2:247407/1‑250 (MQ=255)                                         ccACAGTCTGCTGGCGGTATTTGCGCTGCTGGCACCCTTTTACCTTAAGGTTCGGGAAGGCTGGTTCATCCCGGTTGAGGCGCTACAAGGAATGGTGCTGGGTTATTTGCGCCACCTACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGt                                                                                                                                                                                          <  1:304662/251‑1 (MQ=255)                                                                   ggtgggCAACCTTTGTCTTTAAGGTTCGGGAAGGTTGGTTCATTCCGGCTTATGCGCTACAAGGAATGGTGCTGGTTGATTGGGCCCACTTACGTGCCGTGATGCTGCCACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCTCTGGCGTTTCCGCTTGCCTATTCTGTTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTAccc                                                                                                                                                                <  2:287878/247‑1 (MQ=255)                                                                                             ccGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTACTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTCGCGTTTCCGCTTGCCTATTCTAGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACACCGCTGTTCGTTGGTCATc                                                                                                                                      >  1:43560/1‑251 (MQ=255)                                                                                                gAAGGGTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCa                                                                                                                                   <  1:247407/251‑1 (MQ=255)                                                                                                       ggTTCATTCCGGCTGATGCGCTACAAGGAAGGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGAt                                                                                                                             <  1:391532/250‑1 (MQ=255)                                                                                                                                aGGAATGGTGCTGGGTTATTGGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATc                                                                                                   <  1:289440/251‑1 (MQ=255)                                                                                                                                 ggAAGGGTGCTGGGTTATTTGAGCCACATACTTGCGGATATGGTGACACCCGCCGGTGTGCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCGTGCAGATCCAGTTTCATCg                                                                                                  <  2:41774/251‑1 (MQ=255)                                                                                                                                                         aacaTACTTGCCGATATGCTGAAACCCACCGGTGTTCCCCTGACCTGGCCATGCAGCTGGCGTTTCCGCTTGCCTATTCTGGTACCCCAAAAGTGCAAGCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCAGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAAAACCTTGCAGATCAAGTTACATCGGCTTATTAAGCATCCGGTTGAAt                                                                           >  2:252957/2‑250 (MQ=255)                                                                                                                                                                             gccccccGCCGGTTTTCCCCTGCTCTTGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCAcc                                                      <  2:343729/247‑1 (MQ=255)                                                                                                                                                                             cccccccGCCGGTTTGCCCCCGCGCTCGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTGATCTGCATCGCATTATTTGTCTGGTCGGTATGGATTCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTTCAGATACAGTTTCATCTGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCAcc                                                      <  2:293760/247‑1 (MQ=255)                                                                                                                                                                                acCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGCAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGATTACTAAAAAGGCAAAAATCACCttt                                                   >  2:179623/1‑251 (MQ=255)                                                                                                                                                                                 cccGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTc                                                  <  1:384026/251‑1 (MQ=255)                                                                                                                                                                                      cgGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAACAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCt                                                 >  2:217082/1‑247 (MQ=255)                                                                                                                                                                                      cgGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCt                                                 <  1:217082/247‑1 (MQ=255)                                                                                                                                                                                                     ctGGCCATGCCGCTGGCCTTTCCGCTTGCCTATTCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCTACGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCAt                                <  2:118337/249‑1 (MQ=255)                                                                                                                                                                                                                                 ccTATTCTTGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCTCCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGt  <  2:177182/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CAAAACCGATCGCCCGCGCTTTTGGGCATCGTGGTTTTACCCACAGTCTGCTGGCGGTATTTGCGCTGCTGGCAACCTTTTACCTTAAGGTTCCGGAAGGCTGGTTCATTCCGGCTGATGCGCTACAAGGAATGGTGCTGGGTTATTTGAGCCACATACTTGCCGATATGCTGACACCCGCCGGTGTTCCCCTGCTCTGGCCATGCCGCTGGCGTTTCCGCTTGCCTATCCTGGTTCCCCAAAAGGGCAACCAACTGGAACGTTTTATCTGCATGGCATTATTTGTCTGGTCGGTATGGATGCCCCATTCATTACCCGAGAACAGCGCTGTTCGTTGGTCATCGCAAATGATCAATACCTTGCAGATCCAGTTTCATCGGCTTATTAAGCATCAGGTTGAATACTAAAAAGGCAAAAATCACCTTTCTGGAATAAGCAATTCCATTTGAATATAAGAGCCAGCTCACAGTTCTGT  >  NC_000913/1810395‑1810869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑