Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,603,367	G→A	P293S (CCG→TCG)	ftsY ←	signal recognition particle receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,603,367	0	G	A	100.0%	163.7 / NA	47	P293S (CCG→TCG)	ftsY	signal recognition particle receptor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/31);  total (16/31)

TAGCTGCCTGAATGGCGTCGAAGATAACAGAGGCGGAATCCGCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGGCGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCAGCTCCTCAAACAGATCATCGTCGATTTTTTTACCGCGGAACAGGCTGATAAATCCGGAACC  >  NC_000913/3603130‑3603613                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       tAGCTGCGTGAATGGCGTCGAAGATAACAGAGGCGGAATCCGCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCTCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCTCCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTc                                                                                                                                                                                                                                            <  1:276038/250‑1 (MQ=255)          gAATGGCGTCGAAGATAACAGAGTCTGAATCCGCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTCACCCCAGACCTGAAGCTGTTCAACCGCACCTTCACGGAAAGTATCCCCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACCCCGTTGACGCCCACCATCAGGATCACAATCGACGCTTTGCCTTCAACATTCa                                                                                                                                                                                                                                    <  2:222831/249‑1 (MQ=255)                   cGAAGATAACAGAGGCGGAATCCGCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCg                                                                                                                                                                                                                         >  2:193315/1‑251 (MQ=255)                   cGAAGATAACAGAGGCGGAATCCGCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCg                                                                                                                                                                                                                         >  1:394412/1‑251 (MQ=255)                          aaCAGAGGCGGAATCCGCCCCGGTATGCTGGGCAATCCCCGGAATATTGTGGCGCTGACCCAAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCg                                                                                                                                                                                                                  <  2:167110/251‑1 (MQ=255)                                  cGGAATCCGCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAAtc                                                                                                                                                                                                          <  1:193315/251‑1 (MQ=255)                                        ccGCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGcc                                                                                                                                                                                                     <  1:412695/250‑1 (MQ=255)                                          gCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCAt                                                                                                                                                                                                  >  1:88125/1‑251 (MQ=255)                                               ggTATTCTGGGCAATCACCGGATTATTGTTGCGCTGCCCCCGTCCCTGAAGCTGTTCACCCGCAGCTGCACGGAAAGTACCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCTTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCATCATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCtctt                                                                                                                                                                                             <  2:75835/251‑1 (MQ=255)                                                gTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCtcttc                                                                                                                                                                                            <  1:133005/251‑1 (MQ=255)                                                      tGGGCAATCCCCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCAtct                                                                                                                                                                                                <  2:21396/241‑1 (MQ=255)                                                      tGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCAtct                                                                                                                                                                                                >  1:21396/1‑241 (MQ=255)                                                              cACCGGAATAGTGGTGCGCTGACCCCAGCCCTGAAGCTGTTCAACCGCAGCTGCACGTAAAGTATCACCCGCCGCCGGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCAGCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTGCGCCAGAAGCGCGCCCATCTCTTCTTTCAGCAGGCCa                                                                                                                                                                               <  2:392014/250‑1 (MQ=255)                                                                 cGGAATATTGTTGCGCTGACCCCAGCCCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATaga                                                                                                                                                                           <  1:308814/251‑1 (MQ=255)                                                                      tatTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGcc                                                                                                                                                                      >  1:239506/1‑251 (MQ=255)                                                                        ttgttgCGCTGACCCCAGACATGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGACTCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTc                                                                                                                                                                    >  1:148632/1‑251 (MQ=255)                                                                          gttgCGCTGACCCCAGACCTGAAGCTTTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTGCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCgg                                                                                                                                                                  <  1:67765/251‑1 (MQ=255)                                                                            tgCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCg                                                                                                                                                                >  1:89306/1‑251 (MQ=255)                                                                               gcTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCATTCAAAATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCCGGCCATAGAGAGCCTCGGCgcc                                                                                                                                                              >  1:400334/1‑248 (MQ=255)                                                                                            cTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGc                                                                                                                                                >  1:110361/1‑251 (MQ=255)                                                                                              gAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCCCCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCgg                                                                                                                                              <  1:181665/251‑1 (MQ=255)                                                                                              gAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCg                                                                                                                                               <  1:120288/250‑1 (MQ=255)                                                                                                 gCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGAt                                                                                                                                           >  2:164727/1‑251 (MQ=255)                                                                                                  cTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCGACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGATCATCGACTTTCGCCATAATCTCGCTCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGACGTCACGAAGCTGCTTGCGGGATg                                                                                                                                          >  1:89117/1‑251 (MQ=255)                                                                                                    gTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATgcg                                                                                                                                        <  1:42309/251‑1 (MQ=255)                                                                                                       cAACCGCAGCTGCACGGAAAGTATCCCCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGt                                                                                                                                                               <  1:79550/225‑1 (MQ=255)                                                                                                       cAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGt                                                                                                                                                               >  2:79550/1‑225 (MQ=255)                                                                                                               gCTGCACGGAAAGTATCCCCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAg                                                                                                                             <  2:154704/251‑1 (MQ=255)                                                                                                                    aCGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACt                                                                                                                                                                                                                      >  1:20693/1‑157 (MQ=255)                                                                                                                    aCGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACt                                                                                                                                                                                                                      <  2:20693/157‑1 (MQ=255)                                                                                                                          aGTATCACCCGCCGCCAGCATCACCGATTTGCCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATGCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATaa                                                                                                                  <  1:227847/251‑1 (MQ=255)                                                                                                                               cccccGCCGCCATCATCCCCGATTTCCCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCTTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTa                                                                                                             <  2:233095/249‑1 (MQ=255)                                                                                                                                      cgccAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACAtt                                                                                                                                                                                                                                      <  1:190524/123‑1 (MQ=255)                                                                                                                                      cgccAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACAtt                                                                                                                                                                                                                                      >  2:190524/1‑123 (MQ=255)                                                                                                                                               cACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCcaca                                                                                              <  1:136952/250‑1 (MQ=255)                                                                                                                                                 ccGATTTCCCCTGCTGCTCAAACTGACGCGCCAGCTTACCAAGCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCacac                                                                                             <  2:89306/249‑1 (MQ=255)                                                                                                                                                 ccGATTTACCCTGATGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGGTTTTCCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTGCCGTCAGATTGGTGATAATTTTACGTGTGGGTTCcac                                                                                               <  2:383398/247‑1 (MQ=255)                                                                                                                                                       tACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATc                                                                                      <  1:327664/250‑1 (MQ=255)                                                                                                                                                                    aaaCTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTTAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAgct                                                                        <  1:337677/251‑1 (MQ=255)                                                                                                                                                                             cgcCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCCCAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCa                                                               <  2:409335/251‑1 (MQ=255)                                                                                                                                                                             cgcCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCa                                                               <  2:156432/251‑1 (MQ=255)                                                                                                                                                                                       ccAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACTCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCAGCTCCTCaaa                                                     <  1:196255/251‑1 (MQ=255)                                                                                                                                                                                               cgtGGTTTTACCCACACCGTTGACGCCCACCATTAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCTGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAAGTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCAGCTCCTCAAGCAGaccat                                             <  2:83483/251‑1 (MQ=255)                                                                                                                                                                                                   gTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCAGCTCCTCAAACAGATCAtcgtc                                         >  1:310164/1‑251 (MQ=255)                                                                                                                                                                                                                         ccaccaTCAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCAGCTCCTCAAACAGATCATCGTCGATTTTTTTACCGCGGAACAgg                   >  1:1165/1‑251 (MQ=255)                                                                                                                                                                                                                                cAGGATCACAAACGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCAGCTCCTCAAACAGATCATCGTCGATTTTTTTACCGCGGAACAGGCTGATaa            <  2:110361/251‑1 (MQ=255)                                                                                                                                                                                                                                          aaCGACGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCAGCTCCTCAAACAGATCATCGTCGATTTTTTTACCGCGGAACAGGCTGATAAATCCGGAAcc  <  1:26581/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       TAGCTGCCTGAATGGCGTCGAAGATAACAGAGGCGGAATCCGCCCCGGTATGCTGGGCAATCACCGGAATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCAGGATCACAAACGGCGCTTTGCCTTCAACATTCAGCGGCTCATCGACTTTCGCCAGAATCTCGCCCATCTCTTCTTTCAGCAGGCCATAGAGCGCCTCGGCGTCACGAAGCTGCTTGCGGGATGCGCCTTCCGTCAGATTGGTGATAATTTTACGTGTGGTTTCCACACCCACATCGGCGATCAAAAGCTGCTCTTCCAGCTCCTCAAACAGATCATCGTCGATTTTTTTACCGCGGAACAGGCTGATAAATCCGGAACC  >  NC_000913/3603130‑3603613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑