Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,423	G→A	V219V (GTC→GTT)	yedJ ←	putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,423	0	G	A	100.0%	151.5 / NA	46	V219V (GTC→GTT)	yedJ	putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/19);  total (27/19)

CGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCG  >  NC_000913/2032179‑2032665                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   cgcTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCt                                                                                                                                                                                                                                              <  2:322593/251‑1 (MQ=255)      cAGTGATTCTCACCTACTCCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCAtt                                                                                                                                                                                                                                         <  2:216519/251‑1 (MQ=255)                    tACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGACAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGtt                                                                                                                                                                                                                           >  2:2480/1‑251 (MQ=255)                     acacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTc                                                                                                                                                                                                                          >  1:130384/1‑251 (MQ=255)                     acacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGGGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTGCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCAGCTATCACCTTGTGATCAACACCTTCATTCCCCCCCGCCAGTTc                                                                                                                                                                                                                          <  2:166669/251‑1 (MQ=255)                      cacCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACCGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCg                                                                                                                                                                                                                         >  2:17845/1‑251 (MQ=255)                                       cccaACGTTTTAACATAATAAATTTGCAGCATTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGTTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTGAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACCCCGTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCAt                                                                                                                                                                                                        <  2:345808/250‑1 (MQ=255)                                         caACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATaa                                                                                                                                                                                                      <  1:185842/251‑1 (MQ=255)                                             gTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAACCTc                                                                                                                                                                                                  >  1:87156/1‑251 (MQ=255)                                              ttttAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCg                                                                                                                                                                                                 >  2:218952/1‑251 (MQ=255)                                                   aCATCATAAATTTGCAGCAGTTTCTACAGCATTGCCTGTGCGGCATTCCCGGTGCTGTACGTATCGGTTACTGAT‑ATATTGTCCTGCATCGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCAAGCGCTCAGCCCGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTa                                                                                                                                                                                             >  2:332141/1‑250 (MQ=255)                                                                           tcCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGcc                                                                                                                                                                      >  1:385822/1‑249 (MQ=255)                                                                            ccAGCATTGCCTGTGCGGCTTTCCCGTTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCAGCTGAGAGGGCGGACAGTTTAACATAATTCCAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCa                                                                                                                                                                     <  2:77672/249‑1 (MQ=255)                                                                            ccAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCNAgc                                                                                                                                                                   >  2:377797/1‑251 (MQ=255)                                                                                cATTGCCTGTGAGGCATTCCCGGGTCTTTATGAATCGGTTA‑TGATTATTTTTTCCTGCCTATATTTCCCCGGCCATCTGGGATGGCCGACAGTTTAACATAATTACAGCCATTTAGGTCCACGCGCTCAGCCAGCGGATGAAACCGCATCTATCACCTTGTGATCAACCCCTTCATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAgctgct                                                                                                                                                               <  2:310942/251‑1 (MQ=255)                                                                                 aTTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTACCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCtt                                                                                                                                                              <  1:17845/251‑1 (MQ=255)                                                                                   tGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGc                                                                                                                                                            >  1:91925/1‑251 (MQ=255)                                                                                      cTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCctc                                                                                                                                                         >  1:123942/1‑251 (MQ=255)                                                                                        gtgCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCtt                                                                                                                                                       <  1:214217/251‑1 (MQ=255)                                                                                                      gTGCTGTATGAATCGGGTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGt                                                                                                                                         >  1:353674/1‑251 (MQ=255)                                                                                                                    ggTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCAATCTGAGAGGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCCCCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCa                                                                                                                           <  1:225419/251‑1 (MQ=255)                                                                                                                                  ttttCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTg                                                                                                              >  1:78670/1‑251 (MQ=255)                                                                                                                                     tCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGa                                                                                                            >  1:384138/1‑250 (MQ=255)                                                                                                                                       cTGCATAGATTTCACCGGCCATCTGAGATGGCAGACAGGGTAACATAATTACAGCCATTTAGCGCCACAAGCGCAGCCAGCGGATGAAAACGCATCTATCACCATGTGATCAAAACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGAGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCGTCAGCAACTTAGTCTGGAAAt                                                                                                         >  1:339718/1‑251 (MQ=255)                                                                                                                                           aTAGATTTCCCCGGCCATCTGAGATGGCCAACAGGTTACAATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATc                                                                                                     <  2:152228/251‑1 (MQ=255)                                                                                                                                             aGATTTCACCGGCCATCTGGGATGGCCGACAGGTTAACATAATTCCAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTTCCACCTTGTGATCAACACCTTCATTCACCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGGTTGCGGTAGCGTCAGCAACTTAGTCTGGAAATGATCCa                                                                                                   <  2:37672/251‑1 (MQ=255)                                                                                                                                                   cACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGt                                                                                             >  2:124964/1‑251 (MQ=255)                                                                                                                                                      cggccacttGAGATGGCCGCCAGGTTAACATAATTACAGCAATTTAGGGCCACGCGCTCAGCCAGCGGATGAAACCGCATCTATCTCCTTTTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGt                                                                                                                                         <  1:31246/196‑1 (MQ=255)                                                                                                                                                      cGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGt                                                                                                                                         >  2:31246/1‑204 (MQ=255)                                                                                                                                                       ggCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCCCCCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTTTGATCAACACCTTCATCCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCg                                                                                         <  1:38118/251‑1 (MQ=255)                                                                                                                                                              tGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTatc                                                                                   >  1:36165/1‑250 (MQ=255)                                                                                                                                                                agaTGGCCGACAGGTTAACATAATTACAGCCATTGAGGGCCACGCGCGCAGCCAGCGGATGAAAACGCATCTATCCCCTGGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTatcatc                                                                                <  1:145439/251‑1 (MQ=255)                                                                                                                                                                              ttAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCt                                                                  >  2:109013/1‑251 (MQ=255)                                                                                                                                                                               tAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCt                                                                  <  1:323973/250‑1 (MQ=255)                                                                                                                                                                               tAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAACGTTTATCATCAAGCGGGCGATGCtg                                                                 >  1:192373/1‑251 (MQ=255)                                                                                                                                                                                                 ttAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCAcc                                               >  2:109530/1‑251 (MQ=255)                                                                                                                                                                                                         aCGCGCTCAGCCAGCGGATGAAAACGCATCTATCAACTTGTGATCAACACCTTCATTCTCCCCAGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGTCGATGCTGTGCGAACGGATCTTCACCATCAAAc                                        >  1:137250/1‑250 (MQ=255)                                                                                                                                                                                                           gcgcTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGCTGCTGTGCGAACGGATCTTCACCATCAAACAgc                                     >  2:222978/1‑251 (MQ=255)                                                                                                                                                                                                               tCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGcc                                  >  1:215048/1‑250 (MQ=255)                                                                                                                                                                                                                   ccagcGGATGAAAACGCATCTATCACCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGcc                             <  1:200345/251‑1 (MQ=255)                                                                                                                                                                                                                    cagcGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGccc                            >  2:334358/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaaCGCATCTATCCCCTAGTGTCCAACACCTCCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCt                   <  2:23390/251‑1 (MQ=255)                                                                                                                                                                                                                                   cATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACg             >  2:124010/1‑251 (MQ=255)                                                                                                                                                                                                                                         tCACCTTGTGCTCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCCCGCCCAATGCCCCTGAAACGGCAAac       >  2:211165/1‑251 (MQ=255)                                                                                                                                                                                                                                             cTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACAcgc   >  1:165363/1‑251 (MQ=255)                                                                                                                                                                                                                                               tgtgATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACAcgcg  <  1:124964/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   CGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGAT‑ATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGCAAACACGCG  >  NC_000913/2032179‑2032665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑