Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,320,867	C→T	P249P (CCG→CCA)	yhbX ←	putative hydrolase YhbX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,320,867	0	C	T	100.0%	87.6 / NA	27	P249P (CCG→CCA)	yhbX	putative hydrolase YhbX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/17);  total (10/17)

ATGGCGATACTGGTAACTGCTGTACCATTCTGCCGAAAAGCGGATTGCGAGCTTAGCCAGAAAGTCTGAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTGAGATTGAAAAACGGCGTATAGGTAGCAAATCGCGACGCTAGTATATATGGACTGAACGCTTTTTTAT  >  NC_000913/3320631‑3321111                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     aTGGCGCTACTGGTAACTGCTGTACCATTCTGCCGACAAGCGGTTTGAGAGCTTAGCCAGAAAGTCTGAAATCCTGCTTGCTTAGCCATATTACTAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGCAAGGGGACCCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCg                                                                                                                                                                                                                                        >  2:190804/1‑251 (MQ=255)               agcngctcTACCATTCTGCCTAAAAGCGTATGGCGAGCTTAGCCAGAACGTCTGACATCCTGCTAGATTAGCCATATTAATAATGTTGGCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAAGGGAACCGACAGCGCAGTGTAAGTTGCGCCGCTTATTGCTTGATTAAACGAGTT‑GATCTCTTGTGGTGGTGCTTCAACTTGTAGTGTCGTAGATCGTGTATATCCaggca                                                                                                                                                                                                                          <  2:371216/249‑5 (MQ=255)                      gttccATTCTGCCGAAAAGCGGATTGCGAGCTTAGCCAGAAAGTCTGAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTTTATATCCATACAAAGACa                                                                                                                                                                                                                    <  1:295146/247‑1 (MQ=255)                      gTACCATTCTGCCGAAAAGCGGATTGCGAGCTTAGCCAGAAAGTCTGAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGAc                                                                                                                                                                                                                     <  1:407480/249‑1 (MQ=255)                        aCCATTCTGCCGAAAAGCGGATTGCGAGCTTAGCCAGAAAGTCTGAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACatat                                                                                                                                                                                                                 <  2:282003/251‑1 (MQ=255)                          cATTCTGCCGAAAAGCGGATTGCGAGCTTAGCCAGAAAGTCTGAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTg                                                                                                                                                                                                               >  1:216409/1‑251 (MQ=255)                                                     ttAGCCAGAAAGTCTGAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATc                                                                                                                                                                                    >  2:279191/1‑251 (MQ=255)                                                             aaaGTCTGAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGAACAGACTCCCCCACAATCAACACGt                                                                                                                                                                             >  2:286037/1‑250 (MQ=255)                                                                    gAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTa                                                                                                                                                                        <  1:6302/248‑1 (MQ=255)                                                                       aTCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAAt                                                                                                                                                                   >  1:350730/1‑250 (MQ=255)                                                                                    aGCCATATTAATAATGTTGGCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACTCGTGCAGATTCCGCCACAATCAACACGTAGGTATCAATACCTGTATCCctga                                                                                                                                                     <  1:232835/251‑1 (MQ=255)                                                                                                             tAATTATGACTGTAATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAATCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGAATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATGAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCgg                                                                                                                            >  2:416305/1‑250 (MQ=255)                                                                                                                 tATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGtt                                                                                                                         <  2:218153/250‑1 (MQ=255)                                                                                                                         gcgtGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGTTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTga                                                                                                                <  2:234986/248‑1 (MQ=255)                                                                                                                                    aaCAGAATCAGCTGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAACTACGGCACCGTGTTTGCAATTGAGAGTAATCttt                                                                                                     >  2:343903/1‑251 (MQ=255)                                                                                                                                    aaCAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCttt                                                                                                     <  2:355640/251‑1 (MQ=255)                                                                                                                                              gcagTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCttt                                                                                           <  1:336452/251‑1 (MQ=255)                                                                                                                                                        aaagGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTaaa                                                                                 <  1:274625/251‑1 (MQ=255)                                                                                                                                                          agGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGc                                                                               <  1:361579/251‑1 (MQ=255)                                                                                                                                                                     cAGCGCCGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATCATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTg                                                                     >  2:257997/1‑250 (MQ=255)                                                                                                                                                                      aGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCGTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGGTgag                                                                   <  1:416305/251‑1 (MQ=255)                                                                                                                                                                                                   ttGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTGAGATTGAAAAACGGCGTATAGGTAGCAAATc                                      >  2:283118/1‑251 (MQ=255)                                                                                                                                                                                                    tGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTGAGATTGAAAAACGGCGTATAGGTAGCAAATc                                      <  1:286037/250‑1 (MQ=255)                                                                                                                                                                                                         aaaC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTGAGATTGAAAAACGGCGTATAGGTAGCAAATCGCGACg                                <  2:114511/251‑1 (MQ=255)                                                                                                                                                                                                                   gATCTGTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTGAGATTGAAGAACGGCGTATAGGTAGCAAAGCGCGACGCTAGtata                        <  1:157364/250‑1 (MQ=255)                                                                                                                                                                                                                        gTTTTCTTTGTGCTTCAACTTGTGGTGTCGTAGAGCGTGTATATCCATACAAGGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTGAGATTGAAAAACGGCGTATAGGTAGCAAATCGCGACGCTAGtatat                       <  2:403145/246‑1 (MQ=255)                                                                                                                                                                                                                                        aaCTTGTGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTGAGATTGAAAAACGGCGTATAGGTAGCAAATCGCGACGCTAGTATATATGGACTGAACGCTTTTTTAt  >  1:134169/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     ATGGCGATACTGGTAACTGCTGTACCATTCTGCCGAAAAGCGGATTGCGAGCTTAGCCAGAAAGTCTGAAATCCTGCTTGATTAGCCATATTAATAATGTTGTCGGGGTAATTATGAATGTCATGACTCAAAACAGAATCAGCAGTTAAAGAAAGGGGAACCGACAGCGCAGTGTAAGGTGCGCCGCTTATTGCTTGATTAAAC‑AGTTTGATCTGTTTTCTTTGTGCTTCAACTTGCGGTGTCGTAGAGCGTGTATATCCATACAAAGACATATTGTCGACACGTACAGACTCCCCCACAATCAACACGTAGGTATCAATACCTGTATCCCTGACTGATAATTGAAAATACGGCACCGTGTTTGCAATTGAGAGTAATCTTTGATGCTCTTTCGCTGCTAAAGCAAAATAGTTGAGATTGAAAAACGGCGTATAGGTAGCAAATCGCGACGCTAGTATATATGGACTGAACGCTTTTTTAT  >  NC_000913/3320631‑3321111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑