Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,517,508	G→T	V40V (GTG→GTT)	ortT →	orphan toxin OrtT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,517,508	0	G	T	100.0%	83.4 / NA	25	V40V (GTG→GTT)	ortT	orphan toxin OrtT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/6);  total (19/6)

AAGATGGATAAGGGCAAGTTGCTGTTTGATGATTTTTTCTCAAACTGGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACG  >  NC_000913/1517267‑1517747                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 aaGATGGATAAGGGCAAGTTGCTGTTTGATGATTTTTTCTCAAACTGGAGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGTCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGCTAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAAc                                                                                                                                                                                                                                          >  2:236571/1‑250 (MQ=255)                      cTGTTTGATGATTTTCTCTCAAACTGGTGAAAAAACTTGATGCACGTAAACAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCACTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGAATCCGTTTTTTAAGCGCCTTTCTGCTTGGGGCAACATGGCCGATGAGTTTCTCNGTg                                                                                                                                                                                                                    >  2:381041/1‑251 (MQ=255)                         tttGATGATTTTTTCTCAAACTGGTGAAAAAACTTGATGCACGTCAAAAAATGACTCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGTTTTCTTTCTCACGTTAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGATGGGGCAACATGTCCGATGAGTTTCCCGGTTgcg                                                                                                                                                                                                                 >  2:213429/1‑251 (MQ=255)                                    tttCTCAAACTGGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGtttt                                                                                                                                                                                                       <  2:362032/250‑1 (MQ=255)                                     ttCTCAAACTGGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGGTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTc                                                                                                                                                                                                      >  1:285395/1‑250 (MQ=255)                                            aCTGGTGAAAAAACTTGATGCACGGCAAAAAATGACGCATATTTGCGCGCGATTTATTCATCTGGCTGGACGCCCGTAAATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCCTCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACACGGCCGATGAGTTTCCCGGAGGCGCTGTTGTTTTCACTGtttt                                                                                                                                                                                              >  2:41240/1‑251 (MQ=255)                                                                      aaaaaaTGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGCCGGc                                                                                                                                                                     >  2:275539/1‑250 (MQ=255)                                                                                 cATATTTGCGCGCGTTTTATTCAGCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTGCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACt                                                                                                                                                         <  1:70459/251‑1 (MQ=255)                                                                                              gTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTgg                                                                                                                                            >  1:287099/1‑250 (MQ=255)                                                                                                        tctggattcaCGCCAGTACATGACTCTCTATCAACATACACTTGTTCATTATGCGGTTATGGCAGCAATCGCATTTCTTATCATCTCGGTTCTTTCTCACGATAAGCAACGCATCCGTTTATTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTACCGGTGGGGCTGTCGTTTTTACTGTTTTAAATCTTACTCCCGGGCTTGACGGCACCACGCCGACTT‑TATTCAATTGGATGAATCgtt                                                                                                                                  >  2:45501/10‑248 (MQ=255)                                                                                                                       gTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTACCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGCCGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGc                                                                                                                  >  2:212356/1‑251 (MQ=255)                                                                                                                                           acaTGCTTGTTTTTTATGCGGCTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGc                                                                                               >  1:11889/1‑250 (MQ=255)                                                                                                                                                    ttttttATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATg                                                                                      >  1:164381/1‑250 (MQ=255)                                                                                                                                                      ttttATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑ACTTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGa                                                                                     >  1:232860/1‑249 (MQ=255)                                                                                                                                                                    ggCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTgttgtt                                                                                                                                                                                                         >  2:201924/1‑120 (MQ=255)                                                                                                                                                                    ggCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTgttgtt                                                                                                                                                                                                         <  1:201924/120‑1 (MQ=255)                                                                                                                                                                     gcagcaATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTgg                                                                    >  1:306279/1‑251 (MQ=255)                                                                                                                                                                                       aTCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAag                                                     >  1:183761/1‑248 (MQ=255)                                                                                                                                                                                                         cACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCatat                                  <  1:236571/249‑1 (MQ=255)                                                                                                                                                                                                            gATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACg                             >  1:294705/1‑251 (MQ=255)                                                                                                                                                                                                                 gAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAatt                         >  1:132837/1‑250 (MQ=255)                                                                                                                                                                                                                      gCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAAt                   <  2:316667/251‑1 (MQ=255)                                                                                                                                                                                                                      gCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAAt                   >  1:330511/1‑251 (MQ=255)                                                                                                                                                                                                                                      cgcCTGTCTGGTTGGGTCAACTTGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CAGTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTTGATGCCGCGATTGAAGAGAGCGTCATTTCCCATATGAACGAATTATTAATCGCCCTGAGCGATGAc   <  1:69500/251‑1 (MQ=255)                                                                                                                                                                                                                                       gcCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAGGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGCAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGAcg  >  2:154255/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 AAGATGGATAAGGGCAAGTTGCTGTTTGATGATTTTTTCTCAAACTGGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATAT‑CATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACG  >  NC_000913/1517267‑1517747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑