Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,823,332	C→T	intergenic (+47/‑183)	nadE → / → cho	NH3‑dependent NAD(+) synthetase/excinuclease Cho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,823,332	0	C	T	100.0%	113.2 / NA	33	intergenic (+47/‑183)	nadE/cho	NH3‑dependent NAD(+) synthetase/excinuclease Cho
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/12);  total (21/12)

ACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCC  >  NC_000913/1823088‑1823577                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         aCGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCCCTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATACTTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATg                                                                                                                                                                                                                                                    >  2:27597/1‑251 (MQ=255)   ggCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATgcg                                                                                                                                                                                                                                                  >  1:265294/1‑251 (MQ=255)        aTCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTg                                                                                                                                                                                                                                             >  1:120254/1‑251 (MQ=255)            ggAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAAc                                                                                                                                                                                                                                          <  1:166604/250‑1 (MQ=255)            ggAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCGGGAAAAAGTAATAATTGGCGCATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTg                                                                                                                                                                                                                                             <  2:160891/247‑1 (MQ=255)              aaGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGcc                                                                                                                                                                                                                                       <  1:250394/251‑1 (MQ=255)                gatgatCTCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCtt                                                                                                                                                                                                                                     >  1:3445/1‑251 (MQ=255)                         ccTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCt                                                                                                                                                                                                                            <  2:99346/251‑1 (MQ=255)                               ctGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCtata                                                                                                                                                                                                                         <  2:170311/248‑1 (MQ=255)                                 gCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATaaa                                                                                                                                                                                                                       <  2:156565/248‑1 (MQ=255)                                           gTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTc                                                                                                                                                                                                          >  1:179715/1‑251 (MQ=255)                                                   cGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACAtt                                                                                                                                                                                                  >  2:227966/1‑251 (MQ=255)                                                                     tatCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAAAAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTATGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGcc                                                                                                                                                                               >  1:50135/1‑251 (MQ=255)                                                                                    ggAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCAc                                                                                                                                                                >  1:78928/1‑251 (MQ=255)                                                                                            aaaaCGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTc                                                                                                                                                                                                                                 <  1:215336/179‑1 (MQ=255)                                                                                            aaaaCGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTc                                                                                                                                                                                                                                 >  2:215336/1‑179 (MQ=255)                                                                                                     cTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGACTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTAACAGGCGTAGCACGTCAGACGGCGTAACCt                                                                                                                                               >  1:181223/1‑251 (MQ=255)                                                                                                                gCCAGAACAATAGATAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTG‑T‑CAGCGAc                                                                                                                                  <  2:163300/251‑1 (MQ=255)                                                                                                                                      aTCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCa                                                                                                                                                                 <  1:110221/200‑1 (MQ=255)                                                                                                                                      aTCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCa                                                                                                                                                                 >  2:110221/1‑200 (MQ=255)                                                                                                                                         tGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTcccc                                                                                                         >  2:153445/1‑250 (MQ=255)                                                                                                                                                 gAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCTCGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTac                                                                                                 >  2:324556/1‑251 (MQ=255)                                                                                                                                                        accccggcccccaaatacccGTTTTCGTTGATTTGGGAAAAAAGAAATAATTGGCAAATATTGGATTGGGAGAAAAAGAGTATTTTGTTCACGTCGGAGGCGGGGTGAACGCTTTATCGGCCCTAAAAAACAAGGAAAATTCAATACATGGCAGG‑AGTCAAAAAGGCGTGACGGGGGTAGCACGTCAGAGGGTG‑AACCTTTT‑TTCAGCCACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGgat                                                                                          <  1:135656/233‑1 (MQ=255)                                                                                                                                                                  gCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATATGCCTGACAGGCGTAGCACGACCGACGGGGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAAcc                                                                                  >  2:280473/1‑249 (MQ=255)                                                                                                                                                                          ccGTTTTCGAGTATTTCTGAAAAAAGTAAAAATTTCCATATTTTGGATTGTGAAAAAAAGATTAATTTTTCCACGTGGGACGCGGCGTGACCCCTTTATTCGACTTAAAAAACTATAAAAATTAAATAC‑TTGGAGGGAGTAAAATAGGCATGACAGGCGTAGCTCTTCAGAGGGTGTATCTTTTG‑C‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCGGTTACACTGGATAGATAACCAGCATTCGGa                                                                        <  1:324556/251‑1 (MQ=255)                                                                                                                                                                                            ggAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCg                                                      >  2:162533/1‑251 (MQ=255)                                                                                                                                                                                                              cacaTATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTCTGCAAATTCAATACATTGCAGG‑AGTCGAATAAGCCTGACAGGCGTAGCACGTCAGACGGTGGAAACTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGTGCCAACAGTGGTACGGCGTTTAACTTCTTCGCGGCt                                    >  1:41184/1‑251 (MQ=255)                                                                                                                                                                                                                 atatTGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGACAATTCAATACCTTGCAGG‑AGTCGAATAGGCCTGACAGGCGCAGCACGTCAGACGGGGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTTGGAGTCCACAGTGGTAAGGCGGTTAACTTCTCCGCGGCTCg                                  >  2:75764/1‑250 (MQ=255)                                                                                                                                                                                                                     tGGATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCCGG‑AGTCGAATAGGCCTGACAGGCGGAGCACGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGATTCAAAAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAAttt                             >  2:174101/1‑251 (MQ=255)                                                                                                                                                                                                                      ggATTGTGCGAAAAAGAGTAATTTGTTCACGTCGGATGCGGCGTGAACGCCTTATTCGAACTATAAAAATATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAAAAGTGGGACGGCGTTTAACTTCTCCGCGGCTCGAATTTg                            >  1:310637/1‑251 (MQ=255)                                                                                                                                                                                                                       gATTGTGCGAAAAAGAGTAATTTGTTCCCGTCGGATGCGGCGTGAAAGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTg                            >  1:303209/1‑250 (MQ=255)                                                                                                                                                                                                                                        tAATTTGTCCACGTGGGATGCGGCGTGACCCCGTTATTCGCCCTAAAAAACTATGCAAATCAAAAACATGGCGGG‑ATGCGAAGAGGCTTAAAAGGGGTAGCACGTCAGAGGTTGTA‑CCTTTGGT‑CACCGCCCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTAt           <  1:36449/250‑1 (MQ=255)                                                                                                                                                                                                                                                tCACGTCGGATGCGGCGTGAACAACTTATTCGGCCTATAAAACTATGCAAATTCAATACATTGCCGG‑AGTCGGATAAGCCTGACAGGCGGAGGACGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCCCTTCCCGCCCGTTAAACTGGATAGAAAACCCACATTCGGAGTCAACAGTGGTACGGCGTTTAACTTATCCGCGGCCCGAATTTGAAGCTGGGGCAATTTATGAATATccc  >  2:151686/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         ACGGCCGATCTGGAAGATGATCGCCCTTCTCTGCCAGATGAAGTGGCACTCGGCGTGACCTATGACAATATCGACGACTATCTGGAAGGGAAAAACGTACCTCAACAGGTCGCCAGAACAATAGAGAACTGGTATCTGAAAACCGAACATAAACGCCGTCCGCCAATTACCGTTTTCGATGATTTCTGGAAAAAGTAATAATTTGCACATATTGGATTGTGCGAAAAAGAGTAATTTGTTCACGCCGGATGCGGCGTGAACGCCTTATTCGACCTATAAAACTATGCAAATTCAATACATTGCAGG‑AGTCGAATAGGCCTGACAGGCGTAGCACGTCAGACGGTGTAACCTTTG‑T‑CATCGACCCGCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCC  >  NC_000913/1823088‑1823577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑