Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,445,876	G→A	L89L (CTG→TTG)	rplF ←	50S ribosomal subunit protein L6

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,445,876	0	G	A	100.0%	233.9 / NA	67	L89L (CTG→TTG)	rplF	50S ribosomal subunit protein L6
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/37);  total (30/37)

ACGCACGACTTCGTCGGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGG  >  NC_000913/3445631‑3446119                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aCGCGCGACTTCGTCGGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCa                                                                                                                                                                                                                                                <  2:60689/251‑1 (MQ=255)  cGCACGACTTCGTCGGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAg                                                                                                                                                                                                                                               <  1:206971/251‑1 (MQ=255)     aCGACTTCGTCGGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGctt                                                                                                                                                                                                                                            >  2:259539/1‑251 (MQ=255)       gACTTCGTCGGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGcttct                                                                                                                                                                                                                                          >  2:23899/1‑251 (MQ=255)       gACTTCATCGCCGTCACAAACACCCTTGCCTTTATAAACCTCAGGACGCCGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGCGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGATAATCACATTGCCTTTAACCGCTGCACGGTAACCTAAACCCACCAACTGCAGcttct                                                                                                                                                                                                                                          >  2:167689/1‑251 (MQ=255)        aCTTCGTCGGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGcttctt                                                                                                                                                                                                                                         <  2:301421/251‑1 (MQ=255)            cgtcgGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGt                                                                                                                                                                                                                                      <  1:99645/250‑1 (MQ=255)            cgtcgGCGGAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGt                                                                                                                                                                                                                                      <  2:387571/250‑1 (MQ=255)               cgGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGa                                                                                                                                                                                                                                    >  2:330889/1‑249 (MQ=255)               cgGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGa                                                                                                                                                                                                                                    >  2:313809/1‑249 (MQ=255)                ggCNTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCGTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCTCTGCACTGTAACCTACACCAACCAACTTCAGCTTCTTAGTGAAGc                                                                                                                                                                                                                                 <  2:383335/251‑1 (MQ=255)                     aaCGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTc                                                                                                                                                                                                                             <  1:323936/250‑1 (MQ=255)                       cGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGt                                                                                                                                                                                                                          <  2:143168/251‑1 (MQ=255)                           cccccTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAAACTGGCCGATTCCCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGTCATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCGCATTGCCTTTAACCGCTGCACTGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAaca                                                                                                                                                                                                                      <  2:398653/249‑1 (MQ=255)                            acCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAacac                                                                                                                                                                                                                     >  2:286452/1‑251 (MQ=255)                                        ataAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTg                                                                                                                                                                                                         >  1:66582/1‑251 (MQ=255)                                                 aGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTcagc                                                                                                                                                                                                 >  2:15213/1‑250 (MQ=255)                                                   gacgacGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAgg                                                                                                                                                                                              <  2:192467/251‑1 (MQ=255)                                                      gacgGTAGGAGCGCAGATCCGCTGCAACCTGGCCGCTCAACTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGc                                                                                                                                                                                            >  2:193179/1‑250 (MQ=255)                                                                gcgcAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTAc                                                                                                                                                                                 >  1:279511/1‑251 (MQ=255)                                                                    aGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGACTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGc                                                                                                                                                                             >  2:163110/1‑251 (MQ=255)                                                                            tGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACTATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGccc                                                                                                                                                                     >  2:55222/1‑251 (MQ=255)                                                                            tGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGccc                                                                                                                                                                     >  2:303931/1‑251 (MQ=255)                                                                             gCAACCTGGCCGATCCCCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGccc                                                                                                                                                                     <  2:18338/250‑1 (MQ=255)                                                                                aCCTGGCCGATCACCTTCTTATCAGCGCCTTTCAGCACGATTTCAGGCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCTGCTGATGGTCAGCAGGATGAGAGAATCCCAGAGACAGGTTAATCACATTGCCTTTACCCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAATCCTTCGGTAACACCGATATCCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTTCCCAAcc                                                                                                                                                                 <  1:171536/251‑1 (MQ=255)                                                                                     gCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTg                                                                                                                                                            >  2:49580/1‑251 (MQ=255)                                                                                      ccGATCGCCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGTTGATGGTCAACAGGATGAGAGAAACCCAGATACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTATTAGTGAAGCCTTCGGTAACTCCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCACCCGTCTGc                                                                                                                                                           <  2:246435/251‑1 (MQ=255)                                                                                                  ttATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCg                                                                                                                                                                <  1:160516/234‑1 (MQ=255)                                                                                                  ttATCAGCGCCTTTAAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCg                                                                                                                                                                >  2:160516/1‑234 (MQ=255)                                                                                                   tATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACCCCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGTCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCAc                                                                                                                                                <  1:269300/249‑1 (MQ=255)                                                                                                    aTCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCAcgc                                                                                                                                              <  2:279511/250‑1 (MQ=255)                                                                                                        gcgcCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGAcc                                                                                                                                         <  1:15213/251‑1 (MQ=255)                                                                                                           ccTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGa                                                                                                                                       >  2:323429/1‑250 (MQ=255)                                                                                                                cAGCACGCTTTCAGTCTTAGTCGGACATTCAGCTGGCATACCCGCAGGCAGCTGCTGGTCAACATGATGAGAGAAACCCAGAGACAGGTTAATCACATTTCCTTTATCCTCTGCACGTTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATTACCATTGAGTTCAGCAGGTCACGCGCGGTACCAGCCTGTGCACAACCGTCTGCGTAACCATCACGCGGAACGAAGGTc                                                                                                                                  <  2:1432/250‑1 (MQ=255)                                                                                                                  gCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAgg                                                                                                                               <  1:75043/251‑1 (MQ=255)                                                                                                                  gCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAgg                                                                                                                               >  1:174240/1‑251 (MQ=255)                                                                                                                    aCGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCACCTGATGGTCAACAGGATGAGGGAAACCCAGAGCCAGGTTAATCACATTGCCTTTAACCGTTGCACGGTAACCTACACCAACCAACTCCAGCTTCTTAGTGAAGCCTTCGGTAACCCCGATAACCATTGAGTTCAGCAGGGCACGCCCGGTACCAGCCTGTGCCCAACCGCTTGCGAAACAATCACGCGGACCGAAGGCCAggg                                                                                                                              >  1:317784/1‑250 (MQ=255)                                                                                                                           cAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCt                                                                                                                      <  2:223850/251‑1 (MQ=255)                                                                                                                                 gAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATg                                                                                                                 <  2:201965/250‑1 (MQ=255)                                                                                                                                 gAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGt                                                                                                                >  2:59150/1‑251 (MQ=255)                                                                                                                                   gTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGttt                                                                                                              <  1:313809/251‑1 (MQ=255)                                                                                                                                      ggACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGttatta                                                                                                                         <  2:368790/237‑1 (MQ=255)                                                                                                                                      ggACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGtatta                                                                                                                         >  1:368790/1‑237 (MQ=255)                                                                                                                                      ggACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAAc                                                                                                           >  2:13334/1‑251 (MQ=255)                                                                                                                                        agaTTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGTCACGCGCGGTACCAGCCTGTGCCCATCCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACtt                                                                                                         <  2:353767/249‑1 (MQ=255)                                                                                                                                          aTTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGGGAAGCCGTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCa                                                                                                       <  1:78474/251‑1 (MQ=255)                                                                                                                                               gcagTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCCTTGAGTTAAGCAGGGACCGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAAccgc                                                                                                  >  2:300/1‑248 (MQ=255)                                                                                                                                                 agTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCAt                                                                                                >  2:86512/1‑251 (MQ=255)                                                                                                                                                 agTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCAt                                                                                                <  1:169641/251‑1 (MQ=255)                                                                                                                                                    gATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACCCCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGt                                                                                             <  1:303931/251‑1 (MQ=255)                                                                                                                                                    gATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGt                                                                                             <  1:55222/251‑1 (MQ=255)                                                                                                                                                    gATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAAAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCg                                                                                              <  2:138676/250‑1 (MQ=255)                                                                                                                                                              ggcagCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGACGGTCAGGGCATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACg                                                                                   >  2:30698/1‑251 (MQ=255)                                                                                                                                                               gcagCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACCCCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGa                                                                                  <  2:337089/251‑1 (MQ=255)                                                                                                                                                                           cAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGcc                                                                       <  1:23899/250‑1 (MQ=255)                                                                                                                                                                           cAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCg                                                                      <  1:257078/251‑1 (MQ=255)                                                                                                                                                                               aGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGtttt                                                                  <  1:259539/251‑1 (MQ=255)                                                                                                                                                                                ggATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATACCCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAATTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGttttt                                                                 <  2:281241/251‑1 (MQ=255)                                                                                                                                                                                              cAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGta                                                    >  1:246677/1‑250 (MQ=255)                                                                                                                                                                                                  gaCAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAAc                                               >  2:198080/1‑251 (MQ=255)                                                                                                                                                                                                    cAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCt                                             <  2:51885/251‑1 (MQ=255)                                                                                                                                                                                                       gctAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGAGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTgcc                                          >  1:177547/3‑249 (MQ=255)                                                                                                                                                                                                            tCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTg                                     >  1:53878/1‑251 (MQ=255)                                                                                                                                                                                                                   gCCTTTAACCGCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGAttttt                               <  1:323429/250‑1 (MQ=255)                                                                                                                                                                                                                             gCTGCACGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGcc                    >  2:99371/1‑251 (MQ=255)                                                                                                                                                                                                                                  aCGGTAACCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGc                 >  1:84140/1‑249 (MQ=255)                                                                                                                                                                                                                                       aaCCTACACCAACCAACTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaac           <  1:333824/250‑1 (MQ=255)                                                                                                                                                                                                                                              cccaaccaaATGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCg   <  2:1405/250‑1 (MQ=255)                                                                                                                                                                                                                                               ccaaccaaCTGCAGCTTCTTAGTGAAACCTTCGGTAACACCGATAACCAATGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGACAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTCGATTTTTACGTCAACGCCGGAAGGAACAACGACCgg  >  2:399879/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ACGCACGACTTCGTCGGCGTAACGAACACCCTTGCCTTTATAAGGCTCAGGACGACGGTAGGCGCGCAGATCCGCTGCAACCTGGCCGATCACCTGCTTATCAGCGCCTTTCAGCACGATTTCAGTCTGAGTCGGACATTCAGCAGTGATACCCGCAGGCAGCTGATGGTCAACAGGATGAGAGAAACCCAGAGACAGGTTAATCACATTGCCTTTAACCGCTGCACGGTAACCTACACCAACCAGCTGCAGCTTCTTAGTGAAGCCTTCGGTAACACCGATAACCATTGAGTTCAGCAGGGCACGCGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGG  >  NC_000913/3445631‑3446119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑