Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,380	C→T	Q322Q (CAG→CAA)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,380	0	C	T	100.0%	110.7 / NA	32	Q322Q (CAG→CAA)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/8);  total (24/8)

AACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCG  >  NC_000913/3790137‑3790618                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               aaCTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAat                                                                                                                                                                                                                                         <  1:121265/251‑1 (MQ=255)  aCTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATCTTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAatc                                                                                                                                                                                                                                        >  1:279735/1‑251 (MQ=255)          gCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGAt                                                                                                                                                                                                                                  >  1:323269/1‑249 (MQ=255)                              aCAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATc                                                                                                                                                                                                            >  1:138357/1‑251 (MQ=255)                                 ggTGAGAGATTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTTACATGATTTTACATATTTAAAAGATTATCCACAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCACGAGCGCATATCGTCAAAGCCCTGTTGTCAATCCTCGATAGAGAAACGATGTATAAGGATCGGc                                                                                                                                                                                                         <  2:192970/251‑1 (MQ=255)                                    gagagaGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCTGAATAACTGTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGACCGGCGa                                                                                                                                                                                                       <  2:234147/250‑1 (MQ=255)                                                tCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGcc                                                                                                                                                                                           <  2:347919/250‑1 (MQ=255)                                                 cAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGcc                                                                                                                                                                                           >  2:19753/1‑249 (MQ=255)                                                                aTGAGAGCAGCTATACAAGAGATCAGTACAATTACGAACTATTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAA‑GCGCTGGAATACCATCCCTTGTTCGTGTTAATCCCAGCTAAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCTTCACAGCACTTTTGGAAATCTTCGATATAGAAACGATGGGTAATGATCGGCAAAAGATCGAGGACAGACTGAATCatagcatc                                                                                                                                                                         >  2:23293/1‑244 (MQ=255)                                                                  gagaGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCgccgcc                                                                                                                                                                        >  1:189993/1‑251 (MQ=255)                                                                   agagCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCgccgcc                                                                                                                                                                        >  1:6095/1‑250 (MQ=255)                                                                   agagCAGCTATATAAGAGATCAGCACAATTACGAACTCTATATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCACTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCgccgcc                                                                                                                                                                        >  1:316630/1‑250 (MQ=255)                                                                                                   gAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCg                                                                                                                                       >  1:61710/1‑251 (MQ=255)                                                                                                    aaCTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCACGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGt                                                                                                                                      >  1:222915/1‑251 (MQ=255)                                                                                                     acgctTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGTTCGAACGCATATCGTCAAAGCCCTTTTGGAAATCATCGAGAGAGAAACGATTGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCTCCGCCATCTTGTACCAGGTTTCAAACATCTGACGACCGTa                                                                                                                                     <  2:182057/248‑1 (MQ=255)                                                                                                                cacTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAACTACCTTTa                                                                                                                           >  2:288918/1‑250 (MQ=255)                                                                                                                 acTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAAt                                                                                                                         >  1:150275/1‑251 (MQ=255)                                                                                                                                cATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTaa                                                                                                           >  2:262413/1‑250 (MQ=255)                                                                                                                                 atatTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAg                                                                                                         <  2:138357/251‑1 (MQ=255)                                                                                                                                             ttATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTc                                                                                             >  1:156510/1‑251 (MQ=255)                                                                                                                                               aTCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCa                                                                                           <  1:19753/251‑1 (MQ=255)                                                                                                                                                           aaGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGACGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGCTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACat                                                                               >  1:378563/1‑251 (MQ=255)                                                                                                                                                           aaGGGCTGGAATACCAGCCCTTGTTCATGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACat                                                                               >  1:172023/1‑251 (MQ=255)                                                                                                                                                                     aTACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGAcggc                                                                     <  2:6095/251‑1 (MQ=255)                                                                                                                                                                                tGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATAccc                                                            >  1:218186/1‑249 (MQ=255)                                                                                                                                                                                       gtTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGc                                                    >  2:216056/1‑250 (MQ=255)                                                                                                                                                                                        ttAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCa                                                   >  2:188695/1‑250 (MQ=255)                                                                                                                                                                                         tAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAAt                                                 >  2:171043/1‑251 (MQ=255)                                                                                                                                                                                                    cAGAATAACTTTCCCGGACTGGCCCACACGCCTAGCGTCAAACCCCTTTTGGCAATCATCGATAGAGAAACGATGGGCAATGCTCGGCGAAAGATCGCGGCCAGACTGAATCAGCGCCGCCATCTTCTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGAAATACGGctgccgg                                      >  2:393575/1‑250 (MQ=255)                                                                                                                                                                                                                  cGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTc                         >  2:199335/1‑250 (MQ=255)                                                                                                                                                                                                                      cTGGCCCGAACGCATAGCGTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATAACTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGACGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGCGATTCATGGTGTCAAGc                     >  1:55948/1‑250 (MQ=255)                                                                                                                                                                                                                                        gTCAAAGCCCTTTTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTggcg  >  1:324512/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               AACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCG  >  NC_000913/3790137‑3790618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑