Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,802,144	C→T	E143K (GAA→AAA)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,802,144	0	C	T	100.0%	108.1 / NA	32	E143K (GAA→AAA)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

CCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGT  >  NC_000913/1801901‑1802390                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       cccACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAg                                                                                                                                                                                                                                                 <  2:270722/251‑1 (MQ=255)       ccttaccgtaAATACGTTGCAACATTTTGTTGTTGCTGTACCCACGCCAGTAAGTCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTTACt                                                                                                                                                                                                                                           <  2:33326/248‑1 (MQ=255)                   tACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTCAGCCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCAACTATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGTTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTattg                                                                                                                                                                                                                               >  2:69761/1‑248 (MQ=255)                         gCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGAcgtc                                                                                                                                                                                                                          <  1:117381/250‑1 (MQ=255)                                                   cgccaGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCAt                                                                                                                                                                                               <  2:320650/251‑1 (MQ=255)                                                     ccaGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATca                                                                                                                                                                                             <  1:347767/251‑2 (MQ=255)                                                         tAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGAACGTGCGGACCGCGGCACATATCAACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGCTGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGAGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAACTCCTGCATCCGCtt                                                                                                                                                                                         >  1:263818/1‑251 (MQ=255)                                                          aaGCCCCTGCCGTTTTCATGAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTtc                                                                                                                                                                                        <  1:268308/251‑1 (MQ=255)                                                                     gTTTTCATTAGTTTGAAATGATAGCAGAAACGCATGTTCGGTCCGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGCTGTTTTCGTCAAGAATGGCGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTc                                                                                                                                                                             >  2:178792/1‑251 (MQ=255)                                                                          cATNAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACAt                                                                                                                                                                        <  2:372059/251‑1 (MQ=255)                                                                                           ggCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTg                                                                                                                                                       >  2:26566/1‑251 (MQ=255)                                                                                                    gCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACt                                                                                                                                                                                                                                           <  2:80667/158‑1 (MQ=255)                                                                                                        gTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCa                                                                                                                                           <  1:400420/250‑1 (MQ=255)                                                                                                           cGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAAcgt                                                                                                                                       <  2:153824/251‑1 (MQ=255)                                                                                                                                     cGACATATTCTTCATGGAAGTACAGACCTGGCTTTTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAAc                                                                                                              <  2:89816/250‑1 (MQ=255)                                                                                                                                       aCATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCgg                                                                                                           >  1:78225/1‑251 (MQ=255)                                                                                                                                           atTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCg                                                                                                       <  1:201857/251‑1 (MQ=255)                                                                                                                                                       aGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTg                                                                                           >  2:126581/1‑251 (MQ=255)                                                                                                                                                          aCAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGt                                                                                         >  1:66042/1‑250 (MQ=255)                                                                                                                                                          aCAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGt                                                                                         >  1:126915/1‑250 (MQ=255)                                                                                                                                                           cAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTAt                                                                                       <  2:280465/251‑1 (MQ=255)                                                                                                                                                                       gTCATCATGGGCGATGTTTTCGTCAAGAATGGAGTCTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTTCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgt                                                                            <  1:194152/250‑1 (MQ=255)                                                                                                                                                                         catcatGGGCGATGTTATCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTTGCGAAAGGTTCAAGAGCTTTTCGACAGCTGACTTTCTTCTTAATGACGTCGTCGTTTTTCTCAGCAAGCTACTGCATCGGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAgttgt                                                                         >  2:386431/1‑251 (MQ=255)                                                                                                                                                                             atGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGCTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTa                                                                      >  1:267336/1‑250 (MQ=255)                                                                                                                                                                                              cAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATaa                                                                                                               <  2:92436/192‑1 (MQ=255)                                                                                                                                                                                              cAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATaa                                                                                                               >  1:92436/1‑192 (MQ=255)                                                                                                                                                                                                  aaTGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTgcg                                                >  2:346529/1‑251 (MQ=255)                                                                                                                                                                                                     ggAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGcac                                             >  2:327667/1‑251 (MQ=255)                                                                                                                                                                                                            ttGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGt                                       <  1:327667/250‑1 (MQ=255)                                                                                                                                                                                                                                    aaaGTTTCACGCGCTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCACGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAAGGATCTCCAGACCttcttc              <  1:126581/251‑1 (MQ=255)                                                                                                                                                                                                                                      aGTTTCACGCGCTTTGTGCCAGCTGAATTTCTTCTTAATGACGTCGTATTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTGAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGGTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGt            <  2:295176/251‑1 (MQ=255)                                                                                                                                                                                                                                                gcTTTGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGt  <  2:66042/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       CCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATGGCAGAAACGCATGTTCGGTACGTGCGGACCGCGGCACATATCGACATATTCTTCATGGAAGTACAGACCTGGCTTGTCATCATGGGCGATGTTTTCGTCAAGAATGGAGACTTTGTAGCTCTCCCCACGGTTGGCGAAAGTTTCACGCGCTTCGTGCCAGCTGACTTTCTTCTTAATGACGTCGTAGTTTTTCTCAGCAAGCTCATGCATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGT  >  NC_000913/1801901‑1802390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑