Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,469,561	G→A	S98F (TCT→TTT)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,469,561	0	G	A	100.0%	141.7 / NA	40	S98F (TCT→TTT)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/21);  total (19/21)

GGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTT‑CGCATCACCCGGACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATT‑AAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGC  >  NC_000913/3469320‑3469784                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                ggCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTT‑CGCATCACCCGGACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATT‑AAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGc                                                                                                                                                                                                                        >  1:126037/1‑251 (MQ=255)     cGAACATAACGCCATGGGTTCCTGGCATCATTGCTTTT‑CGCATCACCCGGCCAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATT‑AAAGACGACCTACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGACTTTGAAAAAGATGCGCTGcc                                                                                                                                                                                                                    >  2:252545/1‑251 (MQ=255)           aaaCGCCATGGGTTGCTGGCATCATTGTTTTT‑GGCACCCCCCGGACAGTTAAAAGGGCCTACCCCCCAGGTATTTTTATATCCCGCACCATA‑AAAGACGACAAACCAGGTCTCCCCGCCCTGCTGTTTATCCCAGGCAAC‑TAAAAAAAAATAAGA‑CGGAACAGTCCCCGGTG‑CGGGTGTAGCCAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCtt                                                                                                                                                                                                              <  2:108053/250‑1 (MQ=255)            aaCGCCATGGGTTGCTGGCATCATTGCATTT‑CGCATCACCCGGACAGCTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATT‑AAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGTTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCttt                                                                                                                                                                                                             >  2:28783/1‑251 (MQ=255)                   ggggTTGCTGGCTTC‑TTGCTTTTTCCCTTCCCCCGAAAAGTTAGAAGGGCCTACCCACCAGGATTTTTTATATCCCCCACCATA‑AAAGACGACATCCCAGGTCTGACCGCCCTGCTGTTTATTCCAGGAAAC‑TACAGAAGAATTAGA‑GGAAACAGGGCCCTGTG‑CTGGGGTACCCAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAgg                                                                                                                                                                                                      <  2:69621/250‑1 (MQ=255)                         gCTGGCATCATTTCTTTT‑CTCATCACCCGGACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATCCCGCACCATT‑AAAGACAACAAACCAGGTCTGACCCCCCTGCTGTTTTTTCCAGGCAAC‑TACAGAAGAATAAGA‑CGGAACAGTGCCCTGTG‑CTGGTTTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAgtg                                                                                                                                                                                                <  1:124589/251‑1 (MQ=255)                             gcacttttgttttt‑CGCATCCCCCGAAAAGTTAGAAGAGGCTCCCCCCCAGGCTTTTTTATATACCGC‑CCATTTAAAGACGACATACCGGGTCTGACCGCCCTGCTGTTTATCCCAGGCAAC‑TACAGAAAAATTAGA‑CGAAACAGTGCCCTGGG‑CGGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTAtt                                                                                                                                                                                            <  1:110117/241‑1 (MQ=255)                                     gcgttt‑CGCACCCCCCGGACAGTTAGAGGGGACTCCCCCCCAGGCTTTTTAATATACCACACCATA‑AAAGACAACACACCAGGTCTGCCCGCCCTGTTTTTTATCCCGGGCACC‑TACAGAAAATTTAGA‑GGGAACATTGCCTGGTG‑CTGGGTTAGCAGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAAc                                                                                                                                                                                     <  2:50535/247‑1 (MQ=255)                                                 cACCCGGACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATT‑AAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACCGACTAATTTc                                                                                                                                                                          >  2:100403/1‑250 (MQ=255)                                                      ggACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATT‑AAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCagcagc                                                                                                                                                                    >  1:307975/1‑251 (MQ=255)                                                           gatAGAAGAGGCTACCCACCAGGCTTTTTTATAGTCCGAACTTAA‑AAAGGCGACATCCCGGGTGGGACCCCCCTACTATTTATTCCAGATACA‑TACA‑AAAAATTAAAACGAGACAGTCCCGTGTG‑CTTGTGTAGCTAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGATCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTg                                                                                                                                                               <  1:52307/249‑1 (MQ=255)                                                                                       ttATATACCGCACAATT‑AAAGACGACATAACAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGACGAACCGCCATTAGCCGGCGAATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCAGCTCTTTTCGCAGGAACGTGTATTGCCAACCTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGt                                                                                                                                   >  2:297926/1‑251 (MQ=255)                                                                                                   ccATT‑AAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGGACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGc                                                                                                                       >  1:141510/1‑251 (MQ=255)                                                                                                   accagaaaagtggacATACCAGGTCTCACCGCCGTGTTGTTTATTC‑AAGCAACCGAAAGAAAAATTAGA‑CGAAACAGTGCC‑TGTTTATGGTGAATCCAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGTTTCGCTTTAAACGCCACGGGTGAGTTGCATCATTTg                                                                                                                        <  1:153829/239‑1 (MQ=255)                                                                                                                     ccAGGTCTGCCCGCCCTGCTGTTTATTCAGGGAAAC‑AACAGAAAAATTAGA‑CGGAACATTGCCCTGTG‑CGGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGc                                                                                                      <  2:52697/251‑1 (MQ=255)                                                                                                                                  ccctgtttttttttcaaGGCAAC‑AAAAGAAGAATTAGA‑CGGACCGGTCCCCTGTG‑CTGTTGTAGCGAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTagaaga                                                                                         <  2:126037/235‑1 (MQ=255)                                                                                                                                  cccTGTTGTTTATTCCAGGAAAA‑TACAAAAGAATTAGA‑CGAACCAGGGCCTGGTG‑CTGGTGTACCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTagaaga                                                                                         <  1:229752/251‑1 (MQ=255)                                                                                                                                          tttATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGAACCAGTGCCCTGTG‑CTGGTGTACCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACccac                                                                                  <  1:394501/250‑1 (MQ=255)                                                                                                                                               tCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCAACCAGGc                                                                             >  2:283793/1‑250 (MQ=255)                                                                                                                                                       aC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACACACCAGGCATTTTCAt                                                                     >  2:49738/1‑250 (MQ=255)                                                                                                                                                           aaagaagaATTAGC‑CGAACCGGTGCCCTTTG‑TTGGTGTGGCCGATCCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAAt                                                                 <  1:183571/249‑1 (MQ=255)                                                                                                                                                                        a‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTg                                                                                                                                 >  1:88094/1‑174 (MQ=255)                                                                                                                                                                         aggaaacggttccttgtg‑CTGGGGTAGCGAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTg                                                                                                                                 <  2:88094/160‑1 (MQ=255)                                                                                                                                                                            gAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCAttt                                                                         <  1:167174/227‑1 (MQ=255)                                                                                                                                                                            gAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCAttt                                                                         >  2:167174/1‑227 (MQ=255)                                                                                                                                                                                  tGCCCTGTG‑ATGGTGTAGCCAAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCTCCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTg                                            <  1:366061/250‑1 (MQ=255)                                                                                                                                                                                    cccTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCAAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTAGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGat                                          >  1:251062/1‑250 (MQ=255)                                                                                                                                                                                          g‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGAtatttatt                                   <  1:332178/251‑1 (MQ=255)                                                                                                                                                                                             tGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTac                                 >  1:86923/1‑251 (MQ=255)                                                                                                                                                                                                   aGCCGAACCGACATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCtt                           >  1:336083/1‑251 (MQ=255)                                                                                                                                                                                                     ccGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCg                                                                                                                  >  2:278204/1‑162 (MQ=255)                                                                                                                                                                                                     ccGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCg                                                                                                                  <  1:278204/162‑1 (MQ=255)                                                                                                                                                                                                       gAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTTAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCttgtta                       <  1:75171/251‑2 (MQ=255)                                                                                                                                                                                                                  aGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTAGTTATTCCAt             <  2:307975/250‑1 (MQ=255)                                                                                                                                                                                                                  aGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTAGTTATTCCATg            <  1:314042/251‑1 (MQ=255)                                                                                                                                                                                                                  aGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTAGTTATTCCATg            >  1:93130/1‑251 (MQ=255)                                                                                                                                                                                                                        cGTATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTAGTTATTCCATGCTTcc       >  2:28045/1‑250 (MQ=255)                                                                                                                                                                                                                          tATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTg                                                                                                                                 >  2:203513/1‑126 (MQ=255)                                                                                                                                                                                                                          tATTGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTg                                                                                                                                 <  1:203513/126‑1 (MQ=255)                                                                                                                                                                                                                            ttGGATGCAGGCGTATTTGAATTTGAAAAAGATGAGCTGCCGCTCTTTTCGCAGGCAAGTGTATTGCCACACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTAGTTATTCCATGATTCCATTGc  >  2:131487/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                GGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTT‑CGCATCACCCGGACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATT‑AAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAAC‑TACAGAAGAATTAGA‑CGGAACAGTGCCCTGTG‑CTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTACGCTTTAAACGCCACGGGTTAGTTGCATCATTTGCTTTCGCTTTTCCCGGGCAATTTGTAGAAGAAACCCACCAGGCATTTTCATAAATTTTGCCATCGAAAATAACCTGATATTTATTACCACCTTGTTGGTTATTCCATGCTTCCATTGC  >  NC_000913/3469320‑3469784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑