Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,515,342	G→A	G194E (GGA→GAA)	ydcV →	putative ABC transporter membrane subunit YdcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,515,342	0	G	A	100.0%	147.1 / NA	42	G194E (GGA→GAA)	ydcV	putative ABC transporter membrane subunit YdcV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/25);  total (17/25)

CCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCG‑ACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACAT  >  NC_000913/1515095‑1515586                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ccGGGTATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAAt                                                                                                                                                                                                                                                     <  2:362761/251‑1 (MQ=255)        ttGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTgga                                                                                                                                                                                                                                               <  1:363196/250‑2 (MQ=255)                        gTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCg                                                                                                                                                                                                                              >  1:128399/1‑251 (MQ=255)                                  gCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGCTCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatcat                                                                                                                                                                                                                    >  2:202590/1‑251 (MQ=255)                                  gCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatcat                                                                                                                                                                                                                    >  2:310518/1‑251 (MQ=255)                                   ccTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAatcatc                                                                                                                                                                                                                   <  2:344305/251‑1 (MQ=255)                                        aaaaCCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGTCTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCTCCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCGTCCGCTACGTAGTGTTGCCGAATCTCAGTTCCGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTAc                                                                                                                                                                                                              <  1:241021/251‑1 (MQ=255)                                        aaaaCCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTAc                                                                                                                                                                                                              >  1:349983/1‑251 (MQ=255)                                           aCCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGAc                                                                                                                                                                                                           >  1:94686/1‑251 (MQ=255)                                            ccATCAATCTTGAGCCGGGAGTTTTCACCATCGTGGTCGGTCATGCTACTTTTTGTGTAGTGGTGGTGTTTAACAATGTCATCGCCATTTTTCGCCGCGCCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGTCTGGCAAACCTTCCGCTTCGTAGTGTTGCCGAATCTCAGTTCGGTGTTACTTGGAGGAGAAATGCTGGTGTTTGAGGTGTCGTTCGATTAAATCATCGGTACGAcc                                                                                                                                                                                                          <  2:91498/251‑1 (MQ=255)                                                cAATCTGGCTCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTa                                                                                                                                                                                                      <  1:11774/251‑1 (MQ=255)                                                      ggAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAg                                                                                                                                                                                                <  2:263982/251‑1 (MQ=255)                                                        aGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGt                                                                                                                                                                                              <  1:357631/251‑1 (MQ=255)                                                             ggATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTCCGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCACGTCATga                                                                                                                                                                                         <  1:315387/251‑1 (MQ=255)                                                               aTTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaac                                                                                                                                                                                       <  1:300706/251‑1 (MQ=255)                                                                    tCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATgaacgaac                                                                                                                                                                                   <  1:274619/250‑1 (MQ=255)                                                                           cGTGGTGGGTCATGCGCCTTGTTGTGTAGGTGGGGTGTTTAACAATGTCAGCGTTCGGTTTCGCCGCACTGCCGGGAGTCTGGTTGAGGCGTCAGTGGATCTTGGGGCAAATGGTTGGCAAACATTCCGCGACGTAGGGTTGCCGAATCTTAGGGCTGCGTTACGTGCAGGAGAAATGCTGGTGTTTGCCTTGTCGTTCGATGAAATCATCGTTGCGACCTTTACGGCAGGTGATGAACGAAGGGTACCg                                                                                                                                                                            <  2:417063/250‑1 (MQ=255)                                                                             gggtaggtcATGCGACTTTTTGTGTAGTTGTGGTGTTGAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTGGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTGGACGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGGTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTtg                                                                                                                                                                         <  2:62481/246‑1 (MQ=255)                                                                               gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGCTGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTTACGTtgtg                                                                                                                                                                       >  1:413373/1‑251 (MQ=255)                                                                               gtcggtcATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGCCTGAAAGAACGTTACCGttttg                                                                                                                                                                       >  2:329799/1‑248 (MQ=255)                                                                                   gtcATGCGACTTTTTGTGTTGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTg                                                                                                                                                                   <  2:302327/251‑1 (MQ=255)                                                                                     cATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGCAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGtttct                                                                                                                                                                 >  2:11638/1‑248 (MQ=255)                                                                                                  gtgtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTGCTGGCAGGAGAAATGCTGGCGTTTGCCGTGTCGTTCGATGAAATCATCGTTACGACCTTTACGTCAGGGCATGAACGAACGTTACCGGTGTGGTTGCTCAATCAGCTTgga                                                                                                                                                    <  2:169461/251‑2 (MQ=255)                                                                                                    gtAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGc                                                                                                                                                   <  1:210296/250‑1 (MQ=255)                                                                                                      aGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCGTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCGTGGGCGAc                                                                                                                                                <  2:413373/251‑1 (MQ=255)                                                                                                            ggtgTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAAAGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑Tg                                                                                                                                         >  1:5146/1‑251 (MQ=255)                                                                                                                 ttAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGt                                                                                                                                     <  1:122641/250‑1 (MQ=255)                                                                                                                     cAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCgg                                                                                                                                <  1:410826/251‑1 (MQ=255)                                                                                                                       aTGTCATCGCACGTTTTCGCCGCAACTACTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAACCCTTACGATACCAAGTGTTGACGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTCCGGCAGGGCATGAAAGAACGTTTTCGTTGTGGTTGCTCAATCAAATTGGGCCACcgcgcggtataccgggg                                                                                                                               >  2:173448/1‑238 (MQ=255)                                                                                                                         gTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGtaa                                                                                                                             <  2:148432/250‑1 (MQ=255)                                                                                                                                           cgcACCTCCCGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGACCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGCATGAAAGAACGTTACCGTTGTGGTTGCCCAATCAGCTGTGGCGGCCGCGGTG‑TGTACCGGTAACTAACGCGGGGGCActtcc                                                                                                          >  2:49105/1‑248 (MQ=255)                                                                                                                                                        gTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGGTTCCGTTGTGGGTGCTCAATCAGCTTGGGCGACTGCG‑AGATGTACCGGTAACTAACGTGGTGGGACTGCTGGGTATGTTGGt                                                                                              >  2:286320/1‑250 (MQ=255)                                                                                                                                                                                    ggCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGGTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAAATAACGTGGTGGCACTTCTGGTTCTGTTGGTAACAACCTTGCCGATCCCGGGGGcccggg                                                                 >  1:280029/1‑247 (MQ=255)                                                                                                                                                                                       cAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGTaaca                                                                                          <  2:10990/223‑1 (MQ=255)                                                                                                                                                                                       cAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGGTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGTaaca                                                                                          >  1:10990/1‑223 (MQ=255)                                                                                                                                                                                                         ccGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCG‑Ac                                            <  1:310518/250‑1 (MQ=255)                                                                                                                                                                                                                  aGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCG‑ACAATGGTCaa                                   <  2:94686/250‑1 (MQ=255)                                                                                                                                                                                                                   gtgtTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGCATGAACGGAAGTTAACGTTGTGGGTGCTCAATCAGCGTGGGCGACCGCG‑GGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGGAACAACCTTGCCGATCCTGGGGGCCTTGGGGGGAACCCGCGAAGGCGGACAA‑GGTCaaa                                  >  2:365379/1‑249 (MQ=255)                                                                                                                                                                                                                          cGAATCTCAGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGGCCGCG‑TGATGTACCGGTAACTAAAGTGGTGGCACTGCTGGTTATGTTGGTAACAACCTTTCCGATCCTGGGGGCCTGGGGGCTAACCCGCGAAGGCG‑ACAATGGTCAATAACCACTg                          >  2:80950/1‑251 (MQ=255)                                                                                                                                                                                                                                  aGTTCGGCGTTACTGGCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGGCCTGAACGAACGTTACCGTTTTGGGTGCTCAATCAGCCTTGGCGACCGCG‑TGATGGACCGGTAACTAACGGGGGGGCACTGCTGGTTTTGTTGGGAACAACCCTGCCGATCCTGGGGGCCTGGGGGCTAACCCGCGCAGGCG‑ACAATGGGTAATAACCACTGCTACAgg                   >  2:151481/1‑250 (MQ=255)                                                                                                                                                                                                                                                 gCAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCG‑ACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACa   <  1:11638/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cAGGAGAAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCG‑ACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACAt  <  1:80950/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CCGGGCATTGTCACTGGTCTGGCGTTATTAACCGCCTTTAAAACCATCAATCTGGAGCCGGGATTTTTCACCATCGTGGTCGGTCATGCGACTTTTTGTGTAGTTGTGGTGTTTAACAATGTCATCGCCCGTTTTCGCCGCACCTCCTGGAGTCTGGTTGAGGCGTCAATGGATCTTGGGGCCAATGGCTGGCAAACCTTCCGCTACGTAGTGTTGCCGAATCTCAGTTCGGCGTTACTGGCAGGAGGAATGCTGGCGTTTGCCTTGTCGTTCGATGAAATCATCGTTACGACCTTTACGGCAGGTCATGAACGAACGTTACCGTTGTGGTTGCTCAATCAGCTTGGGCGACCGCG‑TGATGTACCGGTAACTAACGTGGTGGCACTGCTGGTTATGTTGGTAACAACCTTGCCGATCCTGGGGGCCTGGTGGCTAACCCGCGAAGGCG‑ACAATGGTCAATAACCACTGATACAGGAATATGCTATGCAACAT  >  NC_000913/1515095‑1515586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑