Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,957,961 G→A intergenic (‑141/‑9) ilvY ← / → ilvC DNA‑binding transcriptional dual regulator IlvY/ketol‑acid reductoisomerase (NADP(+))

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,957,9610GA100.0% 165.3 / NA 49intergenic (‑141/‑9)ilvY/ilvCDNA‑binding transcriptional dual regulator IlvY/ketol‑acid reductoisomerase (NADP(+))
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/30);  total (19/30)

GCCGTGAGAGCGTGGATGGGCTAACGTGCATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGTCC  >  NC_000913/3957718‑3958203
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
gCCGTGAGAGCGTGGATGGGCTAACGTGCATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAAt                                                                                                                                                                                                                                                <  1:87782/248‑1 (MQ=255)
                 gggCTAACGTGCATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCGGGAAGGGTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGCGAATTCAATCTCGCAAACGCGAACCGCACAATAATAAGCACAACATAACGAAGAATCACCATGGCTAACTACTTc                                                                                                                                                                                                                             >  2:116004/1‑250 (MQ=255)
                 gggCTAACGTGCATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTCCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTc                                                                                                                                                                                                                             <  1:165912/250‑1 (MQ=255)
                    cTAACGTGCATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAAt                                                                                                                                                                                                                          >  1:250952/1‑250 (MQ=255)
                        cGTGCATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACt                                                                                                                                                                                                                     <  2:82717/251‑1 (MQ=255)
                         gTGCATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTg                                                                                                                                                                                                                    >  2:148119/1‑251 (MQ=255)
                            cATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAAGTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAAt                                                                                                                                                                                                                 <  1:378776/251‑1 (MQ=255)
                                 cccGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTgcg                                                                                                                                                                                                            >  2:210729/1‑251 (MQ=255)
                                       cgcTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCcagca                                                                                                                                                                                                      <  2:75933/251‑1 (MQ=255)
                                                cAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTgc                                                                                                                                                                                                             >  2:335156/1‑235 (MQ=255)
                                                cAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTgc                                                                                                                                                                                                             <  1:335156/235‑1 (MQ=255)
                                                      ggcggcTTTCCGCCAGATGCAGGAAGGTTTTCATATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGGCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAGCATCACGAAGAATCTCCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTgg                                                                                                                                                                                        <  2:90749/250‑1 (MQ=255)
                                                       gcggcTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCGATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGc                                                                                                                                                                                      <  1:8240/251‑1 (MQ=255)
                                                         ggcTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCaa                                                                                                                                                                                    <  2:198017/251‑1 (MQ=255)
                                                                cGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGc                                                                                                                                                                             >  2:340544/1‑251 (MQ=255)
                                                                         cAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGc                                                                                                                                                                    <  1:329988/251‑1 (MQ=255)
                                                                                gTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATg                                                                                                                                                             <  1:267810/251‑1 (MQ=255)
                                                                                               aaaTCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGgcg                                                                                                                                              <  1:100226/251‑1 (MQ=255)
                                                                                                    cacaGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGc                                                                                                                                         <  1:135135/251‑1 (MQ=255)
                                                                                                         ggANAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACGTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGATGGGCAAATGTCGCTTTATGGGCCGCGATGAAGTCGCCGATGGCGCGAGCTACCt                                                                                                                                    <  2:376404/251‑1 (MQ=255)
                                                                                                             aGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAg                                                                                                                                <  2:250952/251‑1 (MQ=255)
                                                                                                               cccTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAggg                                                                                                                              <  1:357551/251‑1 (MQ=255)
                                                                                                                   cGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGATGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTaa                                                                                                                           >  1:106891/1‑250 (MQ=255)
                                                                                                                           aCGTTGCAAAACTTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAagtagt                                                                                                                  >  2:215244/1‑251 (MQ=255)
                                                                                                                                         gCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCtgtg                                                                                                    >  2:330715/1‑251 (MQ=255)
                                                                                                                                                              atCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAAcccgg                                                                               >  1:210384/1‑248 (MQ=255)
                                                                                                                                                                  aattCCGCCATTAATTTCCTTTCAGATATTGAAATCAATCTCGCAAACTCGAACCGAACAAGAAGAAGCACAACAGCACGAAGAATCCCCATGGCTAACTAGTGCAACACACTTAAGCTGCGCCAGCAGCTGGGACAGCTGGGGCAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAgg                                                                               <  2:372033/245‑1 (MQ=255)
                                                                                                                                                                  aTTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCt                                                                           <  1:116386/251‑1 (MQ=255)
                                                                                                                                                                         cAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATg                                                                    >  1:278635/1‑251 (MQ=255)
                                                                                                                                                                         cAATAAATTTCCTGACATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATGCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGGAGTCATAGTAGGCTGGGGCGCACAGGGTCTGAACCAGGGCCTGAACATg                                                                    >  2:329062/1‑251 (MQ=255)
                                                                                                                                                                          aagaaaTTGCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGAAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGACTGCACATGc                                                                   <  2:387981/248‑1 (MQ=255)
                                                                                                                                                                                      gTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGtc                                                       >  1:20591/1‑251 (MQ=255)
                                                                                                                                                                                      gTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGtc                                                       <  1:4888/251‑1 (MQ=255)
                                                                                                                                                                                       tCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACGATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTCAAAAAGTAGTCATCGTCGGCTGTGGCGCACCGGGTCTGAACCAAGACCTGAACATGCGTGATTCTGGtct                                                      >  2:2380/1‑251 (MQ=255)
                                                                                                                                                                                         atatAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCCCCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCg                                                    <  1:240343/251‑1 (MQ=255)
                                                                                                                                                                                                 aaTTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTcc                                            >  1:284794/1‑251 (MQ=255)
                                                                                                                                                                                                       atcgcGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGTCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCTAGCTACCTTCAGGGTAAAAAATTAGTCATCGTCGGCTGTGGCGCACAGGTTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGct                                      <  1:46357/247‑1 (MQ=255)
                                                                                                                                                                                                        tctcGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCCCAGGGTCTGAACCTGGACCTGAACATGCTTGATTCTGGTCTCGATATCTCATAAGctc                                     >  2:284238/1‑251 (MQ=255)
                                                                                                                                                                                                           cGCAAACGCGAACCGAACAATAAGAAGCACAACATCCCGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGTGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTg                                   <  2:158429/250‑1 (MQ=255)
                                                                                                                                                                                                           cGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTACCTCCTTCAATACGCTGAATCTGCGCCAGCAGCTGTCCCAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATTGCGCGAGCTACCTTCAGTGTAAAAAAGTAGTCATCGTCGTCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGctct                                    <  2:144232/249‑1 (MQ=255)
                                                                                                                                                                                                            ccaacctcaaaccgaacAATAAGAAGCACAACATCACGAAGAATCACCATTGCTAAGTACTTCAATTCACTGAATCTGCGCCAGCATCTGTCACATCGTGGCAAATGTCGCTTTATGTGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTATTCATCGTCGGCTGTTGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCg                                 <  1:366624/242‑1 (MQ=255)
                                                                                                                                                                                                               aaCGCGAACCGAACAATAAGAAGCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTa                               >  2:347128/1‑250 (MQ=255)
                                                                                                                                                                                                                                  gaagCACAACAGCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCCCAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAg           <  2:334827/251‑1 (MQ=255)
                                                                                                                                                                                                                                    agCACAACATCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAgcg         <  2:145067/251‑1 (MQ=255)
                                                                                                                                                                                                                                         aacaTCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGt    >  2:404319/1‑251 (MQ=255)
                                                                                                                                                                                                                                          acaTCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGTc   >  2:278317/1‑251 (MQ=255)
                                                                                                                                                                                                                                          acaTCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGTc   <  2:223211/251‑1 (MQ=255)
                                                                                                                                                                                                                                           caTCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGTcc  <  1:148119/251‑1 (MQ=255)
                                                                                                                                                                                                                                           caTCACGAAGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGTcc  <  1:341529/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  
GCCGTGAGAGCGTGGATGGGCTAACGTGCATCGCCCGCGCGCTGCGGCCAAAATGGCGGCTTTCCGCCAGATGCAGGAAGGTTTTCAGATCGCGTAAATCCACAGGGACAGCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGTCC  >  NC_000913/3957718‑3958203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑