Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,852,000 C→T G262E (GGG→GAG)  ilvB ← acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,852,0000CT98.1% 188.0 / NA 53G262E (GGG→GAG) ilvBacetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/25);  minor base G (0/1);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATC  >  NC_000913/3851758‑3852245
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     
gcgGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCAt                                                                                                                                                                                                                                                >  1:257670/1‑250 (MQ=255)
     tGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATAccc                                                                                                                                                                                                                                            >  1:8637/1‑249 (MQ=255)
     tGCGCTGCCACCCGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCGCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATATTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCa                                                                                                                                                                                                                                           <  2:201252/250‑1 (MQ=255)
        ggttacaccaGCGGGATCAACTGCGCCAGCACGTCATCAAGATCAGCCTGAATCGCCAAGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGGCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAAGATACTTGGGGCTGCGCACGCCGAGCGTCTCCAGCATACCCAGCg                                                                                                                                                                                                                                        >  2:205527/6‑250 (MQ=255)
            ccaccaGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAAc                                                                                                                                                                                                                                   <  2:110084/251‑1 (MQ=255)
                caGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCAGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGAt                                                                                                                                                                                                                               >  1:286794/1‑251 (MQ=255)
                 aGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATg                                                                                                                                                                                                                              <  1:282898/251‑1 (MQ=255)
                       aTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCttt                                                                                                                                                                                                                        <  1:89451/251‑1 (MQ=255)
                           aCTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGc                                                                                                                                                                                                                    >  1:193105/1‑251 (MQ=255)
                             tGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCa                                                                                                                                                                                                                   >  1:56075/1‑250 (MQ=255)
                              gcgcCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCaa                                                                                                                                                                                                                  <  1:50493/250‑1 (MQ=255)
                                 ccAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATg                                                                                                                                                                                                              <  1:306255/251‑1 (MQ=255)
                                   atcACGGCATCAACATCCGCCTGAATCGCCGCGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGACGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGcc                                                                                                                                                                                                            <  2:101170/249‑1 (MQ=255)
                                    gCACGTCATCAACATCCGCCTGAATCGCCACGTGCAGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGccc                                                                                                                                                                                                           <  2:286794/251‑1 (MQ=255)
                                       cGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGa                                                                                                                                                                                                                                <  1:286755/227‑1 (MQ=255)
                                       cGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGa                                                                                                                                                                                                                                >  2:286755/1‑227 (MQ=255)
                                                   ccGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGt                                                                                                                                                                                             <  1:149368/250‑1 (MQ=255)
                                                     gCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAACGTCAt                                                                                                                                                                                          >  1:268858/1‑251 (MQ=255)
                                                     gCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCGCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCAt                                                                                                                                                                                          <  2:303829/251‑1 (MQ=255)
                                                         gAATCCCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCAtggtg                                                                                                                                                                                      >  1:333208/1‑251 (MQ=255)
                                                              gcctcgggCGGGTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCACTCGGACAGAACTGCTCGGTTTTGCCAATCCCCCGGTCCTCAAAACGCGCACCTAGCACCATCAACGAATCCGCCTCCTGCAAAATATAGTGGGTGCTGCGCTCGCCGTTCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGTCAACATGCCCAGCGCCATTAAAGTCATGTTGGTAgg                                                                                                                                                                                 <  2:42331/244‑1 (MQ=255)
                                                                   gTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGtt                                                                                                                                                                            >  1:191477/1‑251 (MQ=255)
                                                                     gCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTgc                                                                                                                                                                          >  2:155026/1‑251 (MQ=255)
                                                                                     accgaGCTCTGCAAGGTCGATTTCGACAAGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCATTTGCGCTTTCTCCGCCAGtt                                                                                                                                                          <  2:313029/247‑1 (MQ=255)
                                                                                       ccAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCa                                                                                                                                                        <  1:56352/251‑1 (MQ=255)
                                                                                       ccAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGAAGGCAGTTGCGCTTTCTCCGCCAGTTcc                                                                                                                                                        >  2:185899/1‑250 (MQ=255)
                                                                                            tctGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCAtt                                                                                                                                                                                                  >  1:181999/1‑204 (MQ=255)
                                                                                            tctGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCAtt                                                                                                                                                                                                  <  2:181999/204‑1 (MQ=255)
                                                                                                  cGGTCGATATCGACATGAATGATTTTGGAATTCGGACAGAACTGCTCGGTTTTGCCAATCGCACGGTCATCAAAACGCGCACCGAGCACTATCACCAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCACACGCCGTGCATCTCCAGCATACCCAGCGACGACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGc                                                                                                                                             <  2:146963/251‑1 (MQ=255)
                                                                                                            cGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTg                                                                                                                                   >  2:275727/1‑251 (MQ=255)
                                                                                                            cGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTg                                                                                                                                   >  2:215921/1‑251 (MQ=255)
                                                                                                             gACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATTCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGa                                                                                                                                  <  2:226128/251‑1 (MQ=255)
                                                                                                                     tgaTTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACAccg                                                                                                                          >  2:104607/1‑251 (MQ=255)
                                                                                                                                 tCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATa                                                                                                               <  2:39418/250‑1 (MQ=255)
                                                                                                                                        gAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCg                                                                                                       >  2:126095/1‑251 (MQ=255)
                                                                                                                                                    tttGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCTCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCa                                                                                            <  2:257670/250‑1 (MQ=255)
                                                                                                                                                               cccGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAatcatc                                                                                >  2:216934/1‑251 (MQ=255)
                                                                                                                                                                 cGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATcgc                                                                              >  2:79815/1‑251 (MQ=255)
                                                                                                                                                                        caAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCg                                                                       >  2:47264/1‑251 (MQ=255)
                                                                                                                                                                          aaaCGCTCCCCGAGCACTATCAACAAATCCGCCTCCTTCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTc                                                                     <  1:201916/251‑1 (MQ=255)
                                                                                                                                                                                gctccGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGTTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCTTTAATCATCGCCGCTGCGTCACGAAt                                                               <  1:288912/248‑1 (MQ=255)
                                                                                                                                                                                       gCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTttct                                                        >  1:401158/1‑251 (MQ=255)
                                                                                                                                                                                         aCTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTttcttc                                                      >  2:119817/1‑251 (MQ=255)
                                                                                                                                                                                                 caaATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAgg                                              >  1:216481/1‑251 (MQ=255)
                                                                                                                                                                                                       cGCCTCCTGCAAAATATAGTTGGTGATGCGCACGCCGTGCATCGCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGAGCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTAACGAATGCTTTCTTCGCTAAAGGCggggg                                        <  2:131845/251‑1 (MQ=255)
                                                                                                                                                                                                               gCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGAAGCGCTTTAGGCAACACGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGggcggcggcc                                >  1:262504/1‑250 (MQ=255)
                                                                                                                                                                                                                  aaaTATAGTTGGTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCtttt                             <  1:104607/251‑1 (MQ=255)
                                                                                                                                                                                                                     tataGTTGGCGCTGCGCACGCCGAGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGCTCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCCGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCCTTTTCt                          >  2:59303/1‑251 (MQ=255)
                                                                                                                                                                                                                            ggTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCTAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCAt                     >  1:139301/1‑249 (MQ=255)
                                                                                                                                                                                                                            ggTGCTGCCCACGTCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATTTTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACTCCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGCCTTTTTCTGCCATa                    <  1:307649/250‑1 (MQ=255)
                                                                                                                                                                                                                             gTGCTGCGCACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGc                  <  1:79815/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gcgcACGCCGTGCATCTCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCt             >  2:10199/1‑251 (MQ=255)
                                                                                                                                                                                                                                     cACGCCGTGCATCTCCAGCATCCCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCtgtg          <  2:240592/251‑1 (MQ=255)
                                                                                                                                                                                                                                             gcatacccagcatacccaGCGACTACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAAtc  <  2:287869/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     
GCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATCGACATGAATGATTTTGGCATTCGGACAGAACTGCTCGGTTTTGCCAATCGCCCGGTCATCAAAACGCGCACCGAGCACTATCAACAAATCCGCCTCCTGCAAAATATAGTTGGTGCTGCGCACGCCGTGCATCCCCAGCATACCCAGCGACAACGGATGCGCTTTTGGCAACATGCCCAGCGCCATTAAAGTCATGGTGGTAGGCAGTTGCGCTTTCTCCGCCAGTTCACGCACCCGTGCGGGCGCATTGATCACACCGCCGCCCAGATAAAGCACCGGGCGTTTGGCAGCGTTAATCATCGCCGCTGCGTCACGAATGCTTTCTTCGCTAAAGGCGGGGGCGGCGGCTTTTTCTGCCATAGCGGGCTGTGTTTCAATC  >  NC_000913/3851758‑3852245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑