Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,439,614	G→A	intergenic (‑105/+2)	yhdN ← / ← rplQ	DUF1992 domain‑containing protein YhdN/50S ribosomal subunit protein L17

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,439,614	0	G	A	100.0%	48.2 / NA	17	intergenic (‑105/+2)	yhdN/rplQ	DUF1992 domain‑containing protein YhdN/50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/11);  total (6/11)

ATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTG‑CCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCC‑AGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAG  >  NC_000913/3439405‑3439840                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     atcatcCAGTATCAATGGTTCGCCGCTTCCTGCCAGTTTATCAAACACACCTGTCGCTTGCGCTTCTGCTATATGGCGCTTTG‑CCCACTGGTCAAGTAACCACATAAATCCTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAAcc                                                                                                                                                                                            <  1:168704/251‑1 (MQ=255)   catcCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTG‑CCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCa                                                                                                                                                                                           <  1:315563/250‑1 (MQ=255)       aaGTATAAATGGTTCGCCGCTACCGGCCAGTGTATAAAACTCACCTTTCGCGTGCGCTTCTGCTATATGGCGCT‑TGACCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTAGGGGTATAAAAAGACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCg                                                                                                                                                                                      <  2:197019/250‑1 (MQ=255)                             ccTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTG‑CCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAAAAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTCCGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGt                                                                                                                                                                <  2:365646/251‑1 (MQ=255)                              cTGCTAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTG‑CCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACTTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGCTGTACACCATCGGCGCGttttc                                                                                                                                                               >  2:85566/1‑248 (MQ=255)                                                              gcTTCTGCTATATGGCGCTCTG‑CCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTAATCTGCAGGAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCATCTGCGCGTTGTCGCCCGCACGGAAGCCCCACTTCAGAATACggg                                                                                                                               >  2:130448/1‑249 (MQ=255)                                                                         atGGCGCTCTG‑CCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGGTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTTTGCTTTCTCTG‑AAAGATCAACCAGCTCGATGTAAGCCATCCGCGCGGTGTCCCCTGCAAGGAACCCACACCTCAGAATACCAGTGTAaccaccc                                                                                                                    >  2:152035/1‑250 (MQ=255)                                                                                   g‑CCCACTGGTCAAGTCACCACATAAATACTCCAGGGGATGAACAAAAGGAGTACAGCTATTGTAGATAAGTGGGGACACACCGGATATAAAAAAACCCGCCGGGGCGGGATTTTTTAAGTTGATTAAAATTACTCTGCGACAGCTTCTGGTTTTTTTG‑AACGATCAACCAGCTGGATGTCAGCCACCGGCGTGTTTTCGCTTGCACGGAAGCCACACTTCAAAATACGAGTGTAACACCCGACACGGCTcc                                                                                                          >  2:44053/1‑250 (MQ=255)                                                                                                                  ccAGGGGATTAACAAAAAGAGTAAATCTATGGTAGATAAGTGGGGATCCTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTT‑AACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCTGGCCC‑AGTTCGTTAAACAg                                                                             <  1:259142/249‑1 (MQ=255)                                                                                                                           gAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAAAGAGGGCCC‑AGTTCGTTAAAAAGTTTTGCCACg                                                                   >  1:3774/1‑250 (MQ=255)                                                                                                                                aaaaGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCC‑AGTTCGTTAAACAGTTTTGCCACGATCTCg                                                             <  1:13328/251‑1 (MQ=255)                                                                                                                                  aaaagTACAGCTATGGTAGATAAGTGGGGATACTACGGGTATAAAAAACCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCAGCGGCGCGTTGTCGCCTGCTCGGAAGCCCCACTTCAGAATACGGTTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCC‑AGTTCGTTAAACAGTTTTGCCACGATCTCGtt                                                           <  2:139535/248‑1 (MQ=255)                                                                                                                                     aggactgCTATTGTAGATAAGTGGGGTTACTACGGGTATAAAAAATCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTGAGGCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCTCGGCTCGCTAAACGCGGGCCC‑AGTTCGTTAAACAGTTTTGCCACGATCTCGTTATc                                                        <  1:229795/245‑1 (MQ=255)                                                                                                                                      gTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGAAGGGGCGGGGATTTTTACGTTGCTTCAAATTAATCTGCAGAAGCTTCTGGTTTCTCTGGAAAG‑TCAAACAGCCCGGTGTAAGCCATAGACGCGTTGTCGCCTGCACGGGAGCCACACGTTAGAAAACGAGTGTAACCAACCGCACGGCACCCGAAAAGCGGGCCCCAGTT‑GTTTAAAACTTTTGCCACGctctaattatc                                                        >  1:178788/1‑243 (MQ=255)                                                                                                                                                                                 aaCCCGCCGTGGTGGGTTGTTTTACGATGCTCCAAATTACTCTTCAGCACCTTCTGCTTTCTCTT‑AACGATCAACCAGCTCGATGTAATCCATCGGCTCGTTGTCGCCTTCACGGAAGCCACACTTCAGAATACGAGTGTAACCAGCGGCACGGCTCGCGAAACGAGGGCCT‑AGTTCGTTAAGCAGTTGTGCCACGAGCTCTTTATCACGAGTACGGGCGAATTCCACACGACGATTAGCAACGCTATCAg            <  2:262935/251‑1 (MQ=255)                                                                                                                                                                                  aCCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCC‑AGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGt           <  1:117563/251‑1 (MQ=255)                                                                                                                                                                                           ggCGGGTTTTTTTACGTTGCTTCAAATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCC‑AGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAag  <  2:289571/251‑1 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     ATCATCCAGTATCAATGGTTCGCCGCTACCTGCCAGGTTATCAAACTCACCTTTCGCTTGCGCTTCTGCTATATGGCGCTCTG‑CCCACTGGTCAAGTAACCACATAAATACTCCAGGGGATGAACAAAAAGAGTACAGCTATTGTAGATAAGTGGGGATACTACGGGTATAAAAAAACCCGCCGGGGCGGGTTTTTTTACGTTGCTTCAGATTACTCTGCAGCAGCTTCTGCTTTCTCTG‑AACGATCAACCAGCTCGATGTAAGCCATCGGCGCGTTGTCGCCTGCACGGAAGCCACACTTCAGAATACGAGTGTAACCACCGGCACGGCTCGCGAAACGCGGGCCC‑AGTTCGTTAAACAGTTTTGCCACGATCTCGTTATCACGAGTACGGGCGAATGCCAGACGACGATTAGCAACGCTATCAGTCTTGGCAAG  >  NC_000913/3439405‑3439840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑