Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,146,389	(T)6→7	coding (132/879 nt)	pflC →	putative pyruvate formate‑lyase 2 activating enzyme PflC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,146,389	1	.	T	100.0%	148.0 / NA	43	F44? (TTT→TTN)	pflC	putative pyruvate formate‑lyase 2 activating enzyme PflC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (21/22);  total (21/22)

CGTGGTGAACGCCGACACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTT‑AAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAA  >  NC_000913/4146139‑4146638                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                          cGTGGTGAACGCCGACACGTTGCGGGAAGCGCAACCGCGCCCACAAGATTATGCCGGGATGGTGGTGAGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCtttttt                                                                                                                                                                                                                                                            >  1:256793/1‑251 (MQ=255) cGTGGTGAACGCCGACACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGAGCGCGTTGCCGGATACAGCGCCTTATTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATACGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCGTTTACAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCtttttt                                                                                                                                                                                                                                                            >  2:304202/1‑251 (MQ=255)     gtgAACGCCGACACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCtttttttaa                                                                                                                                                                                                                                                         >  1:80572/1‑247 (MQ=255)         aCGCCGACACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCt                                                                                                                                                                                                                                                    >  2:365519/1‑251 (MQ=255)             cGACACGGTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTcc                                                                                                                                                                                                                                                <  2:97798/251‑1 (MQ=255)              gACACGTTGCGGGAATCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTCCCGGATACAGCGCCTTCTTTGTCGACCTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGGGTTATTCACCGAATGACGGCGAGGGCATTCGCACGGTGGTCTTTTTTTAAAGGCTGTCCg                                                                                                                                                                                                                                               >  2:363550/1‑251 (MQ=255)               acacGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACGGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGc                                                                                                                                                                                                                                              <  2:235601/251‑1 (MQ=255)                 acGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCAt                                                                                                                                                                                                                                            >  1:17420/1‑251 (MQ=255)                   gTTGCGGGAAGCGAAACCGCGCCTCCAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGACACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGCTGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTAAGGGGGTCTTTTTTTAAGGGCTGTCCGCATCt                                                                                                                                                                                                                                          >  2:289493/1‑251 (MQ=255)                        gggAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGGCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGcc                                                                                                                                                                                                                                     <  2:170716/251‑1 (MQ=255)                                  aCAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCgggggnggc                                                                                                                                                                                                                           >  2:377718/1‑243 (MQ=255)                                  aCAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGc                                                                                                                                                                                                                           >  2:79668/1‑251 (MQ=255)                                     gcgcCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTaa                                                                                                                                                                                                                        <  1:119697/251‑1 (MQ=255)                                        cccACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATcc                                                                                                                                                                                                                     <  2:99927/251‑1 (MQ=255)                                            caAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAg                                                                                                                                                                                                                 <  1:290407/251‑1 (MQ=255)                                              aGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTc                                                                                                                                                                                                               >  2:219956/1‑251 (MQ=255)                                                   aTGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGAtct                                                                                                                                                                                                          >  1:154416/1‑251 (MQ=255)                                                      ccGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTATTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGCTGATGTGGCGCGCATTTTCAACATTCCGCGTTATTCACTGAATGCCGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCCTATATGCCCGTGGTGTTCTAATCCGGATTCGATTTCCg                                                                                                                                                                                                       >  2:399492/1‑251 (MQ=255)                                                              gtggtgCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATc                                                                                                                                                                                               <  1:392596/251‑1 (MQ=255)                                                                 gtgCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCat                                                                                                                                                                                            <  1:273703/251‑2 (MQ=255)                                                                              gATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCagag                                                                                                                                                                               <  1:217033/251‑1 (MQ=255)                                                                               aTACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGCTGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCag                                                                                                                                                                                 >  2:295362/1‑248 (MQ=255)                                                                                   aGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCg                                                                                                                                                                          >  2:305124/1‑251 (MQ=255)                                                                                        cttcttTGGCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGTACAGCGAATAAGCTGTAACGTTGTTGACACGCGCCACGATGATGTGGCTCGCCTTTTCAACGTTCAGCGTTATTCACTGAATGACGGCGAGGGCATTTGTACGGGGGTCTTTTTTTAAAGGCTGTCCGCTTCTTTGTCCGTGGTGTGCGAATCAGGAGTCGATCTCCGGCACATTCCCGCCGGTACGCGGAGAGGCGAAATg                                                                                                                                                                     >  2:47601/1‑251 (MQ=255)                                                                                                  gAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACtgt                                                                                                                                                           >  1:256540/1‑251 (MQ=255)                                                                                                               agaTCCAGGATGAAATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAGACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCCACTCCGGAGTCGATCTCCGGCAAAAACCAGACGGTCCGCAGAGTGCCGAAATGTCTGCACtgcgacaaatgttggc                                                                                                                                              >  2:386625/1‑237 (MQ=255)                                                                                                                     aGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATg                                                                                                                                        >  2:333495/1‑251 (MQ=255)                                                                                                                      ggATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGc                                                                                                                                       <  2:80572/251‑1 (MQ=255)                                                                                                                               catcCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATg                                                                                                                              <  1:8322/251‑1 (MQ=255)                                                                                                                                   cgccgGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGcccc                                                                                                                          <  1:363550/251‑1 (MQ=255)                                                                                                                                         aCAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCggg                                                                                                                    >  1:94833/1‑251 (MQ=255)                                                                                                                                                             gtgtgGAAACGCGTGGCGATGATGTGGGGCGTATTTTCAACATTCAGCGTTATTCACGGAAGGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGTTAATCCGGAGTCGTGCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCTCTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGGGGTTGAACGGATTGGTcg                                                                                                <  2:8826/250‑1 (MQ=255)                                                                                                                                                                        cgccgcGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGACTGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCNGcc                                                                                     >  2:378235/1‑251 (MQ=255)                                                                                                                                                                                            cATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGTTCTCGATATCAGCCTTGACGCTCTGGAACGGGaa                                                                 <  1:336082/251‑1 (MQ=255)                                                                                                                                                                                               tttCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTA‑TCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGtg                                                              <  1:143382/250‑1 (MQ=255)                                                                                                                                                                                                ttCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGCGGCGAAATGTCTGCACTGGGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGtg                                                              >  2:6958/1‑250 (MQ=255)                                                                                                                                                                                                ttCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGtg                                                              >  1:34415/1‑250 (MQ=255)                                                                                                                                                                                                      aTTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAg                                                       <  2:22166/251‑1 (MQ=255)                                                                                                                                                                                                                        aaTGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGACAGATGACATTTTCTTTCGc                                      >  1:240490/1‑250 (MQ=255)                                                                                                                                                                                                                         attaCGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGGAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTGGCGTGATGCGGATGAATGCCCCGCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGAGGACATTTTCTTTCGCAc                                    <  1:289493/248‑1 (MQ=255)                                                                                                                                                                                                                             cGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTcc                                <  2:52001/251‑1 (MQ=255)                                                                                                                                                                                                                                cGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCcggc                             <  2:83491/251‑1 (MQ=255)                                                                                                                                                                                                                                cGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGGTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCcggc                             <  1:295362/251‑1 (MQ=255)                                                                                                                                                                                                                                 gAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCcggcg                            <  1:47757/251‑1 (MQ=255)                                                                                                                                                                                                                                   gggCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCcggcggc                          <  1:103968/251‑1 (MQ=255)                                                                                                                                                                                                                                          cGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTc                    >  1:141056/1‑250 (MQ=255)                                                                                                                                                                                                                                                      tttttt‑AAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTggcg       <  2:17420/250‑1 (MQ=255)                                                                                                                                                                                                                                                       ttttt‑AAAGGATGTGCGCATAATAGCCCGTGGTAGGATAATCCGGAGTCGATCTCCGGCAAAATCTAGACGGTACGCAGAGAGGAGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGAGGATGAAAGCCCCTACGGGGCGCTTGAACGGATTGGTCGCGATATCAGCCTTGACGGTCTGGCACGGACAGTGATGAAAGCTGACATTTTCTTTCGCAGGTCCGGCGGCGGAGCCACGCTATCTggcggg     >  2:245789/1‑250 (MQ=255)                                                                                                                                                                                                                                                           t‑AAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGGCATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGaa  <  1:377718/250‑1 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                          CGTGGTGAACGCCGACACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTT‑AAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAA  >  NC_000913/4146139‑4146638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑