Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,855,005	C→T	A323T (GCC→ACC)	ydjG ←	NADH‑dependent methylglyoxal reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,855,005	0	C	T	100.0%	62.7 / NA	21	A323T (GCC→ACC)	ydjG	NADH‑dependent methylglyoxal reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/10);  total (11/10)

CACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGCCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAAGAATGGAGATTAAATCACTCTGTTTTAATATCCACGCCAGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGC  >  NC_000913/1854776‑1855234                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      cACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCaa                                                                                                                                                                                                                  <  1:203736/251‑1 (MQ=255)          ttGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTc                                                                                                                                                                                                         >  2:341211/1‑251 (MQ=255)                           ttATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTg                                                                                                                                                                                         <  2:39015/250‑1 (MQ=255)                                                    ttGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCcgcg                                                                                                                                                                          >  1:76844/1‑240 (MQ=255)                                                    ttGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCcgcg                                                                                                                                                                          <  2:76844/240‑1 (MQ=255)                                                    ttGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTcgcg                                                                                                                                                               >  1:86467/1‑251 (MQ=255)                                                           aaTCCTGTCTTTTGACGCCACGCTTACACCCCTGTATCTTTTTACATCACATTAACGCGAATATCGCATAACCGATGTTTACTTTCCAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCAGCGACATTTTCGCGTACCTGt                                                                                                                                                        >  2:179214/1‑251 (MQ=255)                                                             tCCTGACTTTTGCCACCACAATTACACCCCTGTATCTTTTTACATCACATTAGCGAGATTATCGCATAACCGATGTTTACTTTCAACATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTc                                                                                                                                                      >  2:166018/1‑251 (MQ=255)                                                                          cgccACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGcc                                                                                                                                         >  2:29981/1‑251 (MQ=255)                                                                                           tGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAAGAATGgaaat                                                                                                                        >  1:252890/1‑248 (MQ=255)                                                                                            gTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAAGAATGGAGAtt                                                                                                                       >  2:323173/1‑251 (MQ=255)                                                                                                      aCATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATATCCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTTATATTCAGTTCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCCCTAAGAATGGAGATTAAATCActct                                                                                                             <  1:104028/251‑1 (MQ=255)                                                                                                               tAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGACACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAAGAATGGAGATTAAATCACTCTGTTTTAat                                                                                                     <  1:88553/250‑1 (MQ=255)                                                                                                                aGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACATATTTTCATCACAGTTTTCACAGAACCAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATGCAGTGCCGCGACATTTTCGCGTTCCTGTTCCGGTGCAGTAGCACCACTAAGAATGGAGATTAAATCACTCTGTTTTAata                                                                                                    <  2:366300/250‑1 (MQ=255)                                                                                                                               aaaCCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAAGAATGGAGATTAAATCACTCTGTTTTAATATCCACGCCAGTGCCag                                                                                    <  2:119081/250‑1 (MQ=255)                                                                                                                                      tGTTTACTTTCAAACTACCCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATCAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAAGAATGGAGATTAAATCACTCTGTTTTAATATCCACGCCAGTGCCTGAGTggg                                                                              >  2:335840/1‑250 (MQ=255)                                                                                                                                                                       gATTTTCATCACAGTCTTCACAGAAACAGTGGTGAATCGTGTGGAGTCTTTACCACTACAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGTGATACAGTGCCGCGACATTTGCGCGTGCCTGTTCCGTTGCAGGAGCCCGACGAAGAATGGAGACTAAATCACTCTGTTTTAATAGCCACGCGGGTTCCAGAGTGGGAATTTTTCACTGATAACGAGCACAAAGTGGCTGc                                            <  2:17225/251‑1 (MQ=255)                                                                                                                                                                          tttCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTCAGAATGGAGATTAAATCACTCTGTTTTAATATCCACGCCAGTGCTAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGacac                                         >  2:91832/1‑251 (MQ=255)                                                                                                                                                                                        tCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACTCTCCAGGGTCTCTGCCATTTCCCTCATCAATTTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGCCATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAATAATGGAGATTAAATCACTCTGTTTTAATATCCACGCCAGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCatat                            <  1:179214/250‑1 (MQ=255)                                                                                                                                                                                                      gTGAATCGTTTTGAGTAATTAACGCTCCAGGGTCTCTGCCATTTCCCCCAACAATTTTTGGTCTGCCGCCGATAAGTTGATATTCAGGGCCGCGACATTTTCGCGTTCCTTTTCCGGTGCAGTAGCCCCACTAAGAATGGAGATTAAATCACTCTGTTTTAATATCCACGCCAGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCa             <  2:252890/251‑1 (MQ=255)                                                                                                                                                                                                                 tGAGTATTTAACGCTCCAGGGTCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAAGAATGGAGATTAAATCACTCTGTTTTAATATCCACGCCAGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGCTTTCACGc  >  2:122689/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      CACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTAGCGCGATTATCGCATAACCGATGTTTACTTTCAAAATAACCTGTTTGAATCACAGATTTTCATCACAGTTTTCACAGAAACAGAGGTGAATCGTGTTGAGTATTTAACGCTCCAGGGCCTCTGCCATTTCCCTCATCAATGTTGCGTCTGCATCCGATAAGTTGATATTCAGTGCCGCGACATTTTCGCGTACCTGTTCCGGTGCAGTAGCCCCACTAAGAATGGAGATTAAATCACTCTGTTTTAATATCCACGCCAGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGC  >  NC_000913/1854776‑1855234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑