Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,429,371	C→T	P146L (CCC→CTC)	fklB →	peptidyl‑prolyl cis‑trans isomerase FklB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,429,371	0	C	T	100.0%	138.5 / NA	40	P146L (CCC→CTC)	fklB	peptidyl‑prolyl cis‑trans isomerase FklB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/19);  total (21/19)

GAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCC  >  NC_000913/4429124‑4429611                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGACAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTTTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGc                                                                                                                                                                                                                                               >  2:404618/1‑251 (MQ=255)           aGCGCGCCGCTGCCGTTCGTCGTCAGCGTTTCCAGGACATTGCAGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGAGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGTTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCg                                                                                                                                                                                                                                     >  2:196912/1‑251 (MQ=255)            gcgcgcCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCgg                                                                                                                                                                                                                                    <  1:78041/251‑1 (MQ=255)             cgcgcCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGt                                                                                                                                                                                                                                   <  2:149801/251‑1 (MQ=255)                    aTGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGc                                                                                                                                                                                                                            >  1:54809/1‑251 (MQ=255)                                           cAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGc                                                                                                                                                                                                     >  2:23155/1‑251 (MQ=255)                                            aGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGc                                                                                                                                                                                                     >  1:257850/1‑250 (MQ=255)                                              gCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACt                                                                                                                                                                                                  <  1:319717/251‑1 (MQ=255)                                                gATGTCTGCTGAAGGTGTGAAATACCTGGAATAAAACGCCAAATAAGAAGGTATGAATAGGACCGAATCTCGCCTGCAGTTCCGCGTTATCAACCAGGGTGACAGCGCAATTCCGGAACGGACCGGCCGCGGTCGTGTTCATGACACCGGTATACTGATCGACTTCACTGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGACTTGATTCAACCACTGa                                                                                                                                                                                                <  2:362114/251‑1 (MQ=255)                                                 aTGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGAc                                                                                                                                                                                               <  1:211237/251‑1 (MQ=255)                                                   ggCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGACGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGATCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGTTTGACACACTGActc                                                                                                                                                                                             >  2:106273/1‑251 (MQ=255)                                                      tgctgAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATCGCCCCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCACTTCCCGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTAAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTAGCTGGATTGAAGCACTCACTCTg                                                                                                                                                                                           >  1:36913/1‑250 (MQ=255)                                                       gctgAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGAt                                                                                                                                                                                         <  1:213604/251‑1 (MQ=255)                                                          gAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGcc                                                                                                                                                                                      <  1:200754/251‑1 (MQ=255)                                                                       aCCTGGAAGAAACCGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGTCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTaa                                                                                                                                                                          >  1:374193/1‑250 (MQ=255)                                                                         cTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATg                                                                                                                                                                       >  1:98396/1‑251 (MQ=255)                                                                            gaagaaAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGa                                                                                                                                                                    <  2:300529/251‑1 (MQ=255)                                                                                         aaaaaGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCg                                                                                                                                                       >  1:215925/1‑251 (MQ=255)                                                                                                      gAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCat                                                                                                                                          <  1:23155/251‑1 (MQ=255)                                                                                                       aaTAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCata                                                                                                                                         <  1:311713/251‑1 (MQ=255)                                                                                                          aGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGATGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATg                                                                                                                                       <  1:196805/250‑1 (MQ=255)                                                                                                                gAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAgcg                                                                                                                                <  2:349763/251‑1 (MQ=255)                                                                                                                         cTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCggcgcagg                                                                                                                       >  1:159172/1‑251 (MQ=255)                                                                                                                             aaTTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGAACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGAGTTTGACAGCTCCGCTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGTTCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCggcgcaggcgc                                                                                                                    >  1:21256/1‑250 (MQ=255)                                                                                                                              aTTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGcat                                                                                                                  <  2:306717/251‑1 (MQ=255)                                                                                                                                              cAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCGGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCGCTCCGTTCAg                                                                                                  <  2:257850/251‑1 (MQ=255)                                                                                                                                                   tGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCAccc                                                                                             >  1:4628/1‑251 (MQ=255)                                                                                                                                                     aaGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTg                                                                                           >  2:410612/1‑251 (MQ=255)                                                                                                                                                          gcAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGtt                                                                                      <  2:98396/251‑1 (MQ=255)                                                                                                                                                                 cGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCAGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTc                                                                               >  1:252352/1‑251 (MQ=255)                                                                                                                                                                 cGGCACGTACCGACCGCGGTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATACCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTc                                                                               >  2:173833/1‑251 (MQ=255)                                                                                                                                                                         cccgaccgCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAActgct                                                                       <  2:215925/250‑1 (MQ=255)                                                                                                                                                                               cgcgTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAAt                                                                 >  2:10966/1‑251 (MQ=255)                                                                                                                                                                                         ttCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAagc                                                        >  1:72842/1‑250 (MQ=255)                                                                                                                                                                                                   cgttAAACTGATCGACGGCCCCGTGTTTGACAGCTCCGTTGCTCGTGCTGAACTCGCTGAATTCCCGGTTAATGGCGCGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTg                                             >  2:170397/4‑251 (MQ=255)                                                                                                                                                                                                           tGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTATGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGACATGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTc                                     >  1:103512/1‑251 (MQ=255)                                                                                                                                                                                                                                aCAGCTCCGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGGGAGGGGAGCAggaat                >  2:411578/1‑248 (MQ=255)                                                                                                                                                                                                                                      ccGTTGCTCGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCa          <  2:121204/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAAccc  <  1:338132/251‑1 (MQ=255)                                                                                                                                                                                                                                              cGTGGTGAACTCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAAcc   <  1:115409/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAATTCCCGGTTAATGGCGTGATCCCTGGCTGGATTGAAGCACTGACTCTGATGCCGGTAGGTTCTAAATGGGAACTGACTATCCCGCAGGAACTGGCATATGGCGAGCGCGGCGCAGGCGCATCCATCCCTCCGTTCAGCACCCTGGTGTTTGAAGTCGAACTGCTGGAAATCCTCTAAGCAGCGCATTCTGTTCCCCTCGAACGAGAGGGGAGCAGGCATTCAGCAATAAACCC  >  NC_000913/4429124‑4429611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑