Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,502	C→T	C171Y (TGC→TAC)	ydhX ←	putative 4Fe‑4S ferredoxin‑like protein YdhX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,502	0	C	T	100.0%	108.6 / NA	35	C171Y (TGC→TAC)	ydhX	putative 4Fe‑4S ferredoxin‑like protein YdhX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/14);  total (21/14)

TTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGG  >  NC_000913/1750302‑1750747                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      ttCGCTAACTGGCTCTGAACCTGTTCAGCATGTTGCGACGGGCTCCTCCATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGACTTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCACATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAgcg                                                                                                                                                                                                     >  2:52749/1‑251 (MQ=255) ttCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAgcg                                                                                                                                                                                                     >  1:26979/1‑251 (MQ=255)   cGCTAACTGTCTCTGAAACTCTTCAGCATGTTGCGACGTGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAAGTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGACAGCCTTTTTCCAGTCGGGACTCAgcgc                                                                                                                                                                                                    <  2:84069/250‑1 (MQ=255)                 gAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAATCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGAACAATGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTTCCAGTCGGGCCTCAGCGCAGAAATCACAttt                                                                                                                                                                                       <  2:367613/249‑1 (MQ=255)                 gAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTAt                                                                                                                                                                                     <  1:109255/251‑1 (MQ=255)                   aaCTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCt                                                                                                                                                                                   <  1:52749/251‑1 (MQ=255)                   aaCTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGCTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCt                                                                                                                                                                                   <  1:18395/251‑1 (MQ=255)                                  tGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGAc                                                                                                                                                                     >  1:319657/1‑250 (MQ=255)                                                             tAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACgg                                                                                                                                          <  2:90378/250‑1 (MQ=255)                                                                 cAAATGTTGACCGAACCGGCGATACAGATGCGGTTGCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACTCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTTTTACGGACaa                                                                                                                                      <  2:163460/250‑1 (MQ=255)                                                                 cAAATGTTGACCGAACCGGCGATACAGATACGGTTTCCCGGCACCTGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACACCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCCGCCGGGACTCAGCGCAGCAATCACATTTATCTGTCACTTTAGTGACTGGGTTAAGGTATCGAATCTGGTACGGACaac                                                                                                                                     >  2:341869/1‑250 (MQ=255)                                                                   aatgtctaCCGATCCGGCGATACAGATGCGGTGTTCCGTCACCAGGTAGCTGATATTGATAGTATTTATGTTGCTGCAACCACTCCTGGATCTCTGGGCTATCTTCTCGACCAAAGATTAATGCGTGTTCCGGGTGGGCGCTGACGCAAATGGGCGGAAGGCCTTTTGCCAGTCGGGACTCAGCGCATAAATCACATTTTTCTATCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAgcg                                                                                                                                   <  1:34110/244‑1 (MQ=255)                                                                   aaTGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAgcg                                                                                                                                   >  2:7804/1‑251 (MQ=255)                                                                       ttGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAgc                                                                                                                                    <  2:393137/246‑1 (MQ=255)                                                                       ttGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAgc                                                                                                                                    >  1:393137/1‑246 (MQ=255)                                                                                 cGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACt                                                                                                                     >  1:203145/1‑251 (MQ=255)                                                                                  ggCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGTACAAGCGCCAATGCAATAACTg                                                                                                                    <  1:90671/251‑1 (MQ=255)                                                                                           gATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGa                                                                                                             >  2:119647/1‑249 (MQ=255)                                                                                                   ttCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCg                                                                                                    <  1:28987/250‑1 (MQ=255)                                                                                                                gTAGCTGATATTGGTAGTATTTATTTTGCTGCAACCAAGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGc                                                                                      >  2:296959/1‑251 (MQ=255)                                                                                                                               aGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCtc                                                                        >  2:152510/1‑250 (MQ=255)                                                                                                                                      atttTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGcc                                                                >  1:34088/1‑251 (MQ=255)                                                                                                                                               cAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGccc                                                       >  1:69461/1‑251 (MQ=255)                                                                                                                                                   cACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGt                                                   >  1:253413/1‑251 (MQ=255)                                                                                                                                                   cACGCCTGAATTTCTGGGCTATCATCGCGACCAAAGATCGATACGAGTTCCGGGTAGGCACTGACGCAAATGGGCGGAAAGCCTTTTGCAAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGAAAGGGTTAAGGAAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCAATGCGATTTTTCTACCCGCTCAATCCCCTGCTCATCACGCAACGACGCCCCCGt                                                   >  2:222644/1‑251 (MQ=255)                                                                                                                                                          gAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCa                                             >  2:310623/1‑250 (MQ=255)                                                                                                                                                            aTTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAAc                                          >  1:133715/1‑251 (MQ=255)                                                                                                                                                              ttCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGt                                        >  1:183752/1‑251 (MQ=255)                                                                                                                                                                           tCGCGACCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCGTCACGCCACGACGCCCCCGTCGGGCAATCGTCGATGCACGGa                             <  2:242500/249‑1 (MQ=255)                                                                                                                                                                                aCCAAAGATCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGCTTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCtt                      >  2:170782/1‑251 (MQ=255)                                                                                                                                                                                       aTCAATGCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTg               <  1:310623/251‑1 (MQ=255)                                                                                                                                                                                         cAATTCGTGTTCCGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGCGTCTCAGCGCAGACATCAAATTTATCTGTCAGTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGGGCAAATGCAATAACTGCAGCCGAGGCACTGCGATTTTTCTACCCGAACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGATCATCGTCGCACTGCt             >  2:207669/1‑251 (MQ=255)                                                                                                                                                                                                 gtTCCGGGTAGGCGCTGAAGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATGTGTCACTTAAGTGACAGCGTTAAGGTAGCGGACCTGGTACGGACAAGAGCCAATGCAATAACAGCAGCCGATGCACTGTGATACTGCTAACCGCACCATCCCCTGCTCATCACGCCACGACGCCCCCTTCGCGCAAACGTCGATGCACGGCTCATCTTCACAGTGCTGACATGAc     >  2:155188/1‑251 (MQ=255)                                                                                                                                                                                                    ccGGGTAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTgg  >  1:348969/1‑251 (MQ=255)                                                                                                                                                                                                    ccGGGTAGGCGCCGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGTCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGTAGCATCTTCGCAGTGCTGACATGACTg   <  1:152510/250‑1 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      TTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATACAGATGCGGTTTCCCGGCACCAGGTAGCTGATATTGATAGTATTTATTTTGCTGCAACCACGCCTGAATTTCTGGGCTATCTTCGCGACCAAAGATCAATGCGTGTTCCGGGCAGGCGCTGACGCAAATGGGCGGAAAGCCTTTTGCCAGTCGGGACTCAGCGCAGAAATCACATTTATCTGCCACTTTAGTGACAGGGTTAAGGTAGCGAACCTGGTACGGACAAGCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGG  >  NC_000913/1750302‑1750747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑