Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,752,116	C→T	R572H (CGT→CAT)	ydhV ←	putative oxidoreductase YdhV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,752,116	0	C	T	100.0%	66.8 / NA	21	R572H (CGT→CAT)	ydhV	putative oxidoreductase YdhV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/6);  total (15/6)

TGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCC‑ACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCG  >  NC_000913/1751886‑1752347                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         tGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAAc                                                                                                                                                                                                                       <  1:332296/250‑1 (MQ=255)           ttCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGcc                                                                                                                                                                                                            >  1:307553/1‑251 (MQ=255)                           aTCTGCGGATCCTTGTCGCATACGAAGGAACAGATAAGATCGTGTTCATTACGCATATCATTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAAAGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTAATCTCTTCGCCGGTGAGCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTCTGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGcac                                                                                                                                                                                              >  2:36466/1‑249 (MQ=255)                                   aTCCTTGTCAAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGACGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGACGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGAGACgg                                                                                                                                                                                    >  2:351271/1‑251 (MQ=255)                                                    aGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGAcc                                                                                                                                                                   >  2:127109/1‑251 (MQ=255)                                                                gATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGACGTTATGCAAACAGACCCGCAACAGCGAc                                                                                                                                                       >  1:65379/1‑251 (MQ=255)                                                                             aCGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATAtt                                                                                                                                          >  2:252494/1‑251 (MQ=255)                                                                               gCATATCTTTGGTTTGCATCAGTTTTACCGCGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATAttt                                                                                                                                         >  1:318764/1‑250 (MQ=255)                                                                                            ttGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCa                                                                                                                            <  2:116834/250‑1 (MQ=255)                                                                                               cATCAGTTTTACCGTGTAGTCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCattga                                                                                                                        <  2:399761/251‑1 (MQ=255)                                                                                                                   cACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGtt                                                                                                    <  2:174606/251‑1 (MQ=255)                                                                                                                                                        tAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGATTCAATCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGCAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCg                                                               >  1:73976/1‑251 (MQ=255)                                                                                                                                                          aTTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGcc                                                             >  1:375077/1‑251 (MQ=255)                                                                                                                                                          aTTTTTCCTGAGTCATCTCTTCGCCGGGGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGCTCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGCTTGGCGTGTAGTTTTTGGTTTAATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGcc                                                             >  2:62275/1‑251 (MQ=255)                                                                                                                                                                ccTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCC‑ACTTc                                                       <  2:98788/250‑1 (MQ=255)                                                                                                                                                                               cGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCC‑ACTTCACGTTGCAGTTTCAAt                                       >  1:12428/1‑251 (MQ=255)                                                                                                                                                                                   ggTGATCGATTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGGAATTATGGCTTTTCAGCGGCGAAGCGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGACATTGATTGGCGTGTCGTTTTTGGATTCATCGTAAGAATCTTCAGAACCAAAAAGTTCTTTCGCCCACT‑CACGTTGCAGTTTCACTGGc                                    >  1:279671/1‑250 (MQ=255)                                                                                                                                                                                    gTGATAGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCC‑ACTTCACGTCGCAGTTTCAATGGCa                                   >  2:271820/1‑250 (MQ=255)                                                                                                                                                                                                             cAAGCGCCAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCC‑ACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATg          <  1:252494/250‑1 (MQ=255)                                                                                                                                                                                                                   ccAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCC‑ACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGc   >  1:261655/1‑251 (MQ=255)                                                                                                                                                                                                                    cAGATCGCCCCGGTAATTATGGCTTTTCAGCGGCGAAACGGTCATTGCCCAGACCCAGATGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCC‑ACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCg  >  1:261943/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         TGTCAGTACCTTCAGTAAAGACCGGGATCTGCGGATCCTTGTCGAATACCCAGGAACAGATAAGATCGTGTTCATTACGCATATCTTTGGTTTGCATCAGTTTTACCGTGTAGGCACGATGCAACGTAAAAATACGCTCTGCAGCTAAATCTAATTTTTCCTGAGTCATCTCTTCGCCGGTGATCGCTTTGAAGAATTTGGCTTCAAGCGCCAGATCGCCCCGGTAATTACGGCTTTTCAGCGGCGAAACGGTCATTGGCCAGACCCAGTTGCACAGGGTGACGGCGTTATGCAAACAGACCCGCAACAGCGACCACTTTGCATATTTAATTTTTGCGTCATTGATTGGCGTGTAGTTTTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCC‑ACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCG  >  NC_000913/1751886‑1752347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑