Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,892,396	C→T	A261V (GCG→GTG)	mdtL →	efflux pump MdtL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,892,396	0	C	T	100.0%	107.7 / NA	32	A261V (GCG→GTG)	mdtL	efflux pump MdtL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/9);  total (23/9)

AAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGCGTAGCCAGCTCGACCTTAGGTATTGCGCAGGTTTGCGGTT  >  NC_000913/3892150‑3892629                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          aaGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCAGTGTTGTTATCACCACCCTCACCCGTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGACGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTg                                                                                                                                                                                                                                       >  1:190797/1‑251 (MQ=255)      aCGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAAt                                                                                                                                                                                                                                  >  1:42482/1‑251 (MQ=255)           cccAGCGGCCCCCGCAGCGTCGGATAAACCACGATAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGTTCCTCTCTTTCGTGAACACGTCACCGGTATTGCGGATGGAAATCATGGGGTTTGAGCGGGGTGAATACGCTACCATTATGGCGCTGACCGCTGGCGTCGGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTa                                                                                                                                                                                                                             <  1:178819/251‑1 (MQ=255)               gCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGcc                                                                                                                                                                                                                         >  2:128578/1‑251 (MQ=255)               gCGGACCCCGCAGCTTCGAATTAACCACGAGAAAATAGCGAGTCGCTGCTTTACCGTCTTTTCCTCAGCCGTGTTCTTAACACCCCCCTCAGCGTTTCGGTGATCCTCACGTTCGTCAACACGTCACCGGTATTGCTGATGGAAAGCATGGGGTTTGAGCGCGGTGAATACCCCACCATTATTGGGCTGCCCGCCGGCGTCAGCATGCCCCTTTCATTCTCCACGCCACTTGTGCTGGGAATTTTCAAGcc                                                                                                                                                                                                                         <  2:163916/251‑1 (MQ=255)                cGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGcc                                                                                                                                                                                                                         >  2:63433/1‑250 (MQ=255)                          aGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTAAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCACCACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTtg                                                                                                                                                                                                               >  1:242782/1‑250 (MQ=255)                             ttCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTtgatg                                                                                                                                                                                                            >  1:270806/1‑250 (MQ=255)                                                 aTAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTc                                                                                                                                                                                       >  2:78826/1‑251 (MQ=255)                                                  tAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCAACCTAAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGGTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTcc                                                                                                                                                                                      >  1:67182/1‑251 (MQ=255)                                                          cGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGgcggcg                                                                                                                                                                              >  2:415894/1‑251 (MQ=255)                                                                       ttttttCCTCAGCCGTGTTGTTATCACCCCCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCCCCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTg                                                                                                                                                                 >  2:141898/1‑251 (MQ=255)                                                                                             aTCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGt                                                                                                                                           <  1:369948/251‑1 (MQ=255)                                                                                              tCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGtt                                                                                                                                          >  1:350193/1‑251 (MQ=255)                                                                                                  caccCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGACATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtctc                                                                                                                                      >  1:274221/1‑251 (MQ=255)                                                                                                             tttCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATc                                                                                                                           >  1:395830/1‑251 (MQ=255)                                                                                                                 ggTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTTAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGc                                                                                                                       <  2:114340/251‑1 (MQ=255)                                                                                                                 ggTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGc                                                                                                                       >  2:17817/1‑251 (MQ=255)                                                                                                                                   cAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTtc                                                                                                      <  1:415894/250‑1 (MQ=255)                                                                                                                                                  aTTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGgtg                                                                                      >  1:401260/1‑251 (MQ=255)                                                                                                                                                           ggAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGa                                                                             <  1:17817/251‑1 (MQ=255)                                                                                                                                                              aaTCATGGGGTTTAAGCGCGGTGAATCCGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACCCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCGGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTc                                                                          >  2:387152/1‑251 (MQ=255)                                                                                                                                                                 cATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAgg                                                                       >  1:70196/1‑251 (MQ=255)                                                                                                                                                                      ggTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTa                                                                  <  1:366947/251‑1 (MQ=255)                                                                                                                                                                      ggTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCAGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGttt                                                                  >  1:256921/1‑250 (MQ=255)                                                                                                                                                                           gAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCCTTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTCCGTTGATGATCACCTCGCAGGTGTTATTCCTGGAGGCGGGGCTCAATCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGcc                                                                                                            >  2:375613/1‑204 (MQ=255)                                                                                                                                                                           gAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGcc                                                                                                            <  1:375613/204‑1 (MQ=255)                                                                                                                                                                                    gAATACGCCCCCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCAATCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAAGGCCGTTTTCAtt                                                    >  2:236205/1‑251 (MQ=255)                                                                                                                                                                                                   aTGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGCGTAg                                      <  1:235413/250‑1 (MQ=255)                                                                                                                                                                                                                         gCATGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGGTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGCGTAGACAGCTCGACCTTAGGTATTgcg               >  1:406443/1‑251 (MQ=255)                                                                                                                                                                                                                           aTGACCGTTTCATTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGCGTGTCCAGCTCGACCTTAGGTATTgcgcc             >  2:416945/1‑250 (MQ=255)                                                                                                                                                                                                                                      aTTCTCCACGCCATTTGTGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGCGTAGCCAGCTCGACCTTCGGTATTGCGCAGGTTTGCGGtt  >  2:65465/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          AAGAAACGCGCCCAGCGGCCCCCGCAGCTTCGGATAAACCACGAGAAAATAGCGAGTCGCTGCTTAACCGTTTTTTCCTCAGCCGTGTTGTTATCACCACCCTCAGCGTTTCGGTGATCCTCACTTTCGTCAACACGTCACCGGTATTGCTGATGGAAATCATGGGGTTTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGCGTAGCCAGCTCGACCTTAGGTATTGCGCAGGTTTGCGGTT  >  NC_000913/3892150‑3892629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑