Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,281,878	C→T	D400N (GAT→AAT)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,281,878	0	C	T	100.0%	72.2 / NA	22	D400N (GAT→AAT)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/17);  total (5/17)

AGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCC  >  NC_000913/4281655‑4282089                                                                                                                                                                                                                                |                                                                                                                                                                                                                    aGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTAc                                                                                                                                                                                          >  1:90002/1‑251 (MQ=255) aGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATGATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTAc                                                                                                                                                                                          <  2:253535/251‑1 (MQ=255)  ggTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGTGCTAACATTCATGATTCTAAAACAAATAAATTATCACTCGTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTTACATGCCAGTATGAGTGGCACAATTTTCTGCAAAGAATTTCTGTAGTGTTAcc                                                                                                                                                                                         <  2:224382/251‑1 (MQ=255)           aaaaaGGTTCCACCTTTTGCACCTTTAACATGTTAGCTCGGGCTAACATTCATTATTCTAAAACAAATAAATTATCTTTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTATCCTCAATCTGCATCTTTCATAATATCTGCAGTAAGCTTCTTATTGAGTGTATGAAGATTATCTACATTTTGTGTCATGCTAGTATTAGAGGCACAATTTTCTTCAAAGAATTTTTGTAGCGTTCGAAGGATATCa                                                                                                                                                                                <  2:343603/251‑1 (MQ=255)                   gCAATCTTTTGCAACTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGGATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATAGCACTATTact                                                                                                                                                                        <  2:174179/251‑3 (MQ=255)                          tttGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCGCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGgaga                                                                                                                                                                   <  1:84743/249‑1 (MQ=255)                              cACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATAtttt                                                                                                                                                             >  2:393646/1‑251 (MQ=255)                                                   ggCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTa                                                                                                                                         >  2:263652/1‑250 (MQ=255)                                                            tCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCAtt                                                                                                                                <  1:104434/250‑1 (MQ=255)                                                                               aaatTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATc                                                                                                            <  1:412993/251‑1 (MQ=255)                                                                                                 aGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATTCCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATagag                                                                                          <  1:217196/251‑1 (MQ=255)                                                                                                        tCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGat                                                                                    <  2:243682/250‑1 (MQ=255)                                                                                                                            tgacgtCAATCTGCATCTTACAGAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCGTCAGTTTGTGACATGCCAGTATGAGTGTCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATTACTATTAATGGGGATATTGTCCTCTCCACGGTCAATGGTAATACCCATTTCCTGGACGCTAGCCTCAACGAAATTAACACGATAGAGATGGATATGAATCATCCAGTCTTTTg                                                                <  2:278476/245‑1 (MQ=255)                                                                                                                                             ttGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATAACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCAAGGCTTTTGTTAAAACAGAACAGTTg                                               >  1:224138/1‑250 (MQ=255)                                                                                                                                              tGCATAATATCTGCAGTAATCTTCATATCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCATGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCCAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTg                                               <  2:398510/249‑1 (MQ=255)                                                                                                                                               gCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTCTCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCt                                                                                                                            >  1:305827/1‑171 (MQ=255)                                                                                                                                               gCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCt                                                                                                                            <  2:305827/171‑1 (MQ=255)                                                                                                                                                                      aTAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCatt                     <  1:393646/251‑1 (MQ=255)                                                                                                                                                                         gCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATCGCTTAAATTGGCattatt                  <  2:220500/251‑1 (MQ=255)                                                                                                                                                                          cGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCAttatta                 <  1:95176/251‑1 (MQ=255)                                                                                                                                                                                      gATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTa     <  1:387780/251‑1 (MQ=255)                                                                                                                                                                                          aTCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATTTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGAGAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGcc  <  2:73453/250‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                    AGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGACTGCATGAAGATTATCCTCAGTTTGTGACATGCCAGTATGAGTGGCACAATCTTCTTCAAAGAATTTCTGTAGTGTTACCAGGATATCACTATTAATGGAGATATTTTCCTCACCACGGAGAATGGTAATACCCATTCCCTGGACGCTAGCCTCATCGAAATTAACACGATAGAGACGGATATTAAACATCCAGGCTTTTGTTAAAACAGAACAGTTGAAATGGCAATTGCTTAAATTGGCATTATTAAAATTAACATTAGCC  >  NC_000913/4281655‑4282089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑