Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,506,702	G→A	A212V (GCC→GTC)	yhfT ←	uncharacterized protein YhfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,506,702	0	G	A	100.0%	139.0 / NA	41	A212V (GCC→GTC)	yhfT	uncharacterized protein YhfT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/21);  total (20/21)

GGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAACCACC  >  NC_000913/3506455‑3506929                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    ggCAATCAACGGCACAAACCCCAGTCCGCCCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACAt                                                                                                                                                                                                                                  <  2:329025/251‑1 (MQ=255)   cAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATc                                                                                                                                                                                                                                 >  2:88600/1‑250 (MQ=255)       cAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATg                                                                                                                                                                                                                             <  2:302088/250‑1 (MQ=255)                              gcataaatgcgtcctaagCCGCCTGAGTAATCAGCGTTTGCGATGGTACCGGCGTTACGGCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATTACGCAgg                                                                                                                                                                                                     <  2:416980/235‑1 (MQ=255)                                  aaaTTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGt                                                                                                                                                                                                 <  2:385718/251‑1 (MQ=255)                                   aaTTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCgcg                                                                                                                                                                                                >  2:129881/1‑249 (MQ=255)                                        tGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGtt                                                                                                                                                                                            >  1:327232/1‑250 (MQ=255)                                         gCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGAGCGTTCTTCAAACACCGAAAGCCAGCTGACATCAATGCCATTTTCATCAAGATGAAGCAGGTCGTGAGTTAt                                                                                                                                                                                          >  2:208952/1‑251 (MQ=255)                                                      gATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCca                                                                                                                                                                               <  1:222308/249‑1 (MQ=255)                                                              aGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCAc                                                                                                                                                                     >  2:301101/1‑251 (MQ=255)                                                              aGCGGTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCAc                                                                                                                                                                     <  1:129881/251‑1 (MQ=255)                                                                              ccGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGAtt                                                                                                                                                     >  2:144418/1‑251 (MQ=255)                                                                                         ccTGCGGCATATGCTTTCTCCAGTGTGCAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTc                                                                                                                                          >  1:108031/1‑251 (MQ=255)                                                                                                aaTATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGtt                                                                                                                                    >  2:56466/1‑250 (MQ=255)                                                                                                      cTTTCTCCAGTGTGAAGCTCGCCACTTAACTGCCAGCCCAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGACGGCGATATAGGGTCAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGCAAGCCCGCTGACATCAATGTCATTTTCATCCCGATGACGCAGCTCGTGAGTGATCGCGATCCCCAGCAGCATCACCTTGCCAATAAAGATTTCGATGGATTCAGGGTTACGAtgt                                                                                                                             >  2:260437/1‑251 (MQ=255)                                                                                                        ttCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAATATTTCGATGGATTCAGGGTTAAGAtgtg                                                                                                                            <  1:128871/250‑1 (MQ=255)                                                                                                            ccAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTggg                                                                                                                          <  1:144418/248‑1 (MQ=255)                                                                                                             cAGTGTGAAGATCGACGCTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGAGTTCATCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATGCAGGGTTAAGATGTGGGAAAt                                                                                                                      <  1:70337/251‑1 (MQ=255)                                                                                                                         cGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACt                                                                                                           >  1:89616/1‑250 (MQ=255)                                                                                                                             actacACTGCAAGCAACAATCTACAGACTGGCAACGGCGGCAATTCATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGCTACTCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCACTGTCCTTTTCGTCACGATGACGCAGGTCGTGAGTTATCGCTATCCCCAGCAGCATCACCATGCCGATCAAGATTTCGTTGGATTCCGGGTTAAGCTGGGGGAAAAAGCGCACGACTAcccc                                                                                                      >  2:390716/5‑249 (MQ=255)                                                                                                                                       cAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGtcatc                                                                                            <  1:301101/251‑1 (MQ=255)                                                                                                                                                   tCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACcaccac                                                                                 <  1:88600/250‑1 (MQ=255)                                                                                                                                                   tCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTGTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACcaccac                                                                                 <  2:155972/250‑1 (MQ=255)                                                                                                                                                     aTGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCAcggc                                                                              >  2:53285/1‑251 (MQ=255)                                                                                                                                                       gCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCAcggcgg                                                                            >  1:85386/1‑251 (MQ=255)                                                                                                                                                          ggCAACGGAGGCAATCAATGCTCCCACGATGGCGCTATAGGGTAAGTTTTTGATAATGCGTGACGTGCGTTCTTCAAAAACCGAAAGCCCGCTGAAATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCAAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGgcggcg                                                                          >  1:63801/1‑250 (MQ=255)                                                                                                                                                           gCAACGGCGGCAATCAATGCACCCACGATGGCGATATAGGGAAAGTTTTTGATAATCCGTGACGTGCGCTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAAGTCGTGCGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCAAACGGGTCATCAGCACCACCACGGCGGCGAt                                                                        >  2:411455/1‑251 (MQ=255)                                                                                                                                                              aCGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATc                                                                       >  2:7609/1‑249 (MQ=255)                                                                                                                                                               cggcggCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAgcg                                                                                                                  >  2:182377/1‑205 (MQ=255)                                                                                                                                                               cggcggCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAgcg                                                                                                                  <  1:182377/205‑1 (MQ=255)                                                                                                                                                                tgcggcAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGAc                                                                   >  2:119878/2‑251 (MQ=255)                                                                                                                                                                      aatcaatGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCt                                                             <  1:221301/251‑1 (MQ=255)                                                                                                                                                                         caatGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATTCCGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCTCGGCGGCGATCAGACTTTGCTTc                                                           <  2:87251/250‑1 (MQ=255)                                                                                                                                                                          aatGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGTCTTTGCTTCCa                                                         <  1:236689/251‑1 (MQ=255)                                                                                                                                                                                cccACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTTCGTTCTTCAAACACCGAAAGCCCGCTTACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGACaa                                                    <  2:108031/250‑1 (MQ=255)                                                                                                                                                                                  cACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACt                                                 <  2:134873/251‑1 (MQ=255)                                                                                                                                                                                                aGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACGCCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGc                                    <  2:98697/250‑1 (MQ=255)                                                                                                                                                                                                     aaGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGCTATCGCGATCCCCAGAAGCATCACCATCCCAATAAGGATTTCGCTGGATTCAGGGTTAAGATGTGGGAAATAGCACACGACTACCACACGGGTCATCAGTACAACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGCAGAAAATCGcaacc                                >  2:18774/1‑249 (MQ=255)                                                                                                                                                                                                                              gTTCTTCAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAacc     >  2:133065/1‑251 (MQ=255)                                                                                                                                                                                                                                tcttcAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTTGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAacca    <  1:188183/250‑1 (MQ=255)                                                                                                                                                                                                                                   tcAAACACCGAAAGCCCGCTGACATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAaccacc  <  1:304751/249‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    GGCAATCAACGGCACAAACCCCAGTCCGCGCATAAATTCTGCCAGAGCCGCCTGATTAATCAGCGTTTGCGATTGTTCCGGCGTTACGCCTGCGGAATATGCTTTCTCCAGTGTGAAGATCGACACTTCACTGCCAGCAAAAATCTTCATGCTGGCAACGGCGGCAATCAATGCTCCCACGATGGCGATATAGGGTAAGTTTTTGATAATCCGTGACGTGCGTTCTTCAAACACCGAAAGCCCGCTGGCATCAATGTCATTTTCATCACGATGACGCAGGTCGTGAGTTATCGCGATCCCCAGCAGCATCACCATGCCAATAAAGATTTCGATGGATTCAGGGTTAAGATGTGGGAAATAGCGCACGACTACCACACGGGTCATCAGTACCACCACGGCGGCGATCAGACTTTGCTTCCAGCCAAACTGGTAGAAAATCGCCACCAGCGGGAACAGTGCAAAAGCTGAAACCACC  >  NC_000913/3506455‑3506929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑