Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,914,009	C→T	A270T (GCA→ACA)	prc ←	tail‑specific protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,914,009	0	C	T	96.3%	84.6 / NA	27	A270T (GCA→ACA)	prc	tail‑specific protease
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (14/12);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCG  >  NC_000913/1913775‑1914240                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        aCATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGccaac                                                                                                                                                                                                                         >  2:185250/1‑249 (MQ=255)                         gaCGAATACGTTCACGGGCCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGTTTTACTCTTTGTTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGtc                                                                                                                                                                                                  >  1:13693/1‑250 (MQ=255)                          aCGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGtcat                                                                                                                                                                                                <  2:235299/251‑1 (MQ=255)                                          gTCAACGTTACCGTACGGGTCTTGGTCCCTTTACCAGCAAGTAAAATTTCCAGACGAACTTTACTGTCATTTGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCAAATTACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGCCAATTTTGACACCAACGCTGATAGCTTTTCTCTACGTTGCCGGACCACCTGCCACCAACGCATTGAAAACGGTGTAGGCATCATCAATTTgcanaac                                                                                                                                                                                >  2:379200/1‑247 (MQ=255)                                                                tGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGa                                                                                                                                                           <  2:391691/250‑1 (MQ=255)                                                                 gggaccTTTACCAGCAGGTAAAATTTACAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACAACATCAAGACGCCAGCCAAGCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGAGAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGGAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACa                                                                                                                                                         <  2:275084/247‑1 (MQ=255)                                                                           cccgcagGTAAAATTTCCAAACGAACTTTACTGCCCATCGGCCCTTTCATTAAGTAAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCATCACCGACAATGTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCAtgtc                                                                                                                                               <  2:366528/248‑4 (MQ=255)                                                                                    aaaaTTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGa                                                                                                                                       <  1:199525/250‑1 (MQ=255)                                                                                     aaaTTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTNaa                                                                                                                                      >  2:380514/1‑250 (MQ=255)                                                                                     aaaTTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGaa                                                                                                                                      <  1:380514/250‑1 (MQ=255)                                                                                                  aaCTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTAc                                                                                                                        <  1:59/251‑1 (MQ=255)                                                                                                   aCTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTAcg                                                                                                                       <  1:19940/251‑1 (MQ=255)                                                                                                       tACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCggg                                                                                                                   >  2:222169/1‑251 (MQ=255)                                                                                                            cccTGCGGCCCTTTAATTAAGGCAACCACATCAGCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTAACCAACACCGACAATCTTTTCACCAACACTGATAGCTCTACTCTTCGCTGTCGGACCGCCTGCCACTATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGATAAACGCATTTCAGTGTTGAACTGTTCGGGATTACGCGGGGAAAg                                                                                                              <  2:192853/251‑1 (MQ=255)                                                                                                                    cccTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTgg                                                                                                      >  2:213918/1‑251 (MQ=255)                                                                                                                     ccTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGt                                                                                                     >  1:406671/1‑251 (MQ=255)                                                                                                                            ttaaGGCAACCACAACATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCTACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCggg                                                                                              >  1:126166/1‑251 (MQ=255)                                                                                                                                ggCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCg                                                                                           >  1:194380/1‑250 (MQ=255)                                                                                                                                                aGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAAGTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGc                                                                          <  1:286021/251‑1 (MQ=255)                                                                                                                                                                 gTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTTAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGGATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCg                                                            >  2:327085/1‑248 (MQ=255)                                                                                                                                                                    caCCATCGGCTGGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATATCTTTACTCTTCGCTGTCGGACCACCTGCCTCCATCGAATTTATAACCGTGTAGTCTTCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGAGTTCACGCGCAAACGCCGGCAtgaccgtcgagaaaa                                                      <  2:403423/250‑15 (MQ=255)                                                                                                                                                                                              cacCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAga                            <  1:339085/251‑1 (MQ=255)                                                                                                                                                                                                   aCAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGacgacg                       >  1:403625/1‑251 (MQ=255)                                                                                                                                                                                                       ttttGTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATg                   >  1:59822/1‑251 (MQ=255)                                                                                                                                                                                                           gTCACCAACGCTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCa                >  1:407062/1‑250 (MQ=255)                                                                                                                                                                                                                     cTGATAGCTTTACTCTTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTAGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAgcg     >  1:310249/1‑251 (MQ=255)                                                                                                                                                                                                                        aTAGCTATACTATTCGCTGTCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGCTTTCACGCGCACACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGACTGGCAAATTTGTAgcggcg  >  2:312307/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                        ACATTTTAACCGCGCGGTCTTCGAGACGAATACGTTCACGGGTCAACGTTACAGTACGGGTCTTGGTCCCTTTACCAGCAGGTAAAATTTCCAGACGAACTTTACTGCCCTTCGGCCCTTTAATTAAGGCAACCACATCATCAAGACGCCAGCCAATCACGTCAACCATCGGCTTGCCTGTTTGACCAACACCGACAATTTTGTCACCAACGCTGATAGCTTTACTCTTCGCTGCCGGACCACCTGCCACCATCGAATTGATAACGGTGTAGTCATCATCCATTTGCAGCACTGCGCCAATACCTTCCAGCGACAAACTCATTTCAGTGTTGAACTGTTCGGTATTACGCGGGGAAAGATAGTTGGTATGCGGGTCGATTTCACGCGCAAACGCCGTCATTGCCAGCGAGAAAACATCTTCGCTGTTGGTTTGCGCCAGACGACGAATGGCAAATTTGTAGCGGCG  >  NC_000913/1913775‑1914240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑